LOCUS       BBa_K1896010             915 bp    DNA     linear       22-Nov-2024
DEFINITION  mGFPuv2 generator
ACCESSION   BBa_K1896010
VERSION     BBa_K1896010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S05355
     misc_feature    62..64
                     /label=start
     misc_feature    776..778
                     /label=stop
     CDS             62..778
                     /label=mGFPuv2
     misc_feature    356..358
                     /label=F99S
     misc_feature    518..520
                     /label=M153T
     misc_feature    548..550
                     /label=V163A
     misc_feature    677..679
                     /label=A206K
     misc_feature    683..685
                     /label=S208L
     CDS             62..778
                     /label=BBa_K1896001
     misc_feature    1..778
                     /label=BBa_S05356
     stem_loop       798..841
                     /label=stem_loop
     misc_feature    787..866
                     /label=BBa_B0010
     terminator      787..866
                     /label=BBa_B0010
     stem_loop       882..901
                     /label=T7 TE
     misc_feature    908..908
                     /label=stop
     polyA_site      902..915
                     /label=polya
     misc_feature    875..915
                     /label=BBa_B0012
     terminator      875..915
                     /label=BBa_B0012
     misc_feature    787..915
                     /label=BBa_S05347
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      361 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      481 actggagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      541 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggctccgttc aactagcaga
      601 ccattatcaa caaaatactc caattggtga tggccctgtc cttttaccag acaaccatta
      661 cctgtcgaca caatctaaac ttttgaaaga tcccaacgaa aagcgtgacc acatggtcct
      721 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataata
      781 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      901 ctttctgcgt ttata
//