BBa_J23105 1 BBa_J23105 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1896002 1 BBa_K1896002 inaZ (GG safe) 2016-10-11T11:00:00Z 2016-10-14T05:25:33Z This part was based on [[Part:BBa_K584021|BBa_K584021]]. This is an improved version of [[Part:BBa_K584021|BBa_K584021]], which encodes the ice nucleating protein gene ''inaZ'' from ''Pseudomonas syringae''. Two BsaI sites have been removed to make the part safe for Golden Gate cloning. false false _2362_ 19009 19009 9 false The many repeats in this gene seem to make this sequence virtually impossible to synthesize, even after changing the codons used. Over-expression of this part at high levels is toxic. false Bob Van Hove, Maarten Van Brempt annotation2495367 1 start range2495367 1 1 3 annotation2495366 1 inaZ range2495366 1 1 3603 annotation2495368 1 stop range2495368 1 3601 3603 annotation2495369 1 repeat region range2495369 1 526 3453 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_S05347 1 BBa_S05347 B0010:B0012 2016-10-11T11:00:00Z 2016-10-12T02:32:06Z false false _9_ 19009 19009 9 false false Bob Van Hove component2495234 1 BBa_B0012 component2495232 1 BBa_B0010 annotation2495232 1 BBa_B0010 range2495232 1 1 80 annotation2495234 1 BBa_B0012 range2495234 1 89 129 BBa_K1896011 1 BBa_K1896011 inaZ (INP) generator 2016-10-12T11:00:00Z 2016-10-14T05:26:33Z todo todo false false _2362_ 19009 19009 9 false todo false Bob Van Hove, Maarten Van Brempt component2499784 1 BBa_S05347 component2499772 1 BBa_S05357 component2499777 1 BBa_K1896002 annotation2499784 1 BBa_S05347 range2499784 1 3673 3801 annotation2499777 1 BBa_K1896002 range2499777 1 62 3664 annotation2499772 1 BBa_S05357 range2499772 1 1 55 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_S05357 1 BBa_S05357 J23105:B0034 2016-10-12T11:00:00Z 2016-10-13T05:35:04Z false false _9_ 19009 19009 9 false false Bob Van Hove component2499763 1 BBa_B0034 component2499761 1 BBa_J23105 annotation2499763 1 BBa_B0034 range2499763 1 44 55 annotation2499761 1 BBa_J23105 range2499761 1 1 35 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1896002_sequence 1 atgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgcgagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacgcaaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatctccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagtatggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagctccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttgatcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaagttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggcagctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggttgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgccggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaagaacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactccatacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccgacataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaagtag BBa_S05347_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23105_sequence 1 tttacggctagctcagtcctaggtactatgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_S05357_sequence 1 tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaa BBa_K1896011_sequence 1 tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgcgagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacgcaaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatctccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagtatggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagctccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttgatcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaagttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggcagctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggttgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgccggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaagaacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactccatacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccgacataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z