##gff-version 3
##sequence-region BBa_K1896012 1 1542
BBa_K1896012	.	engineered_region	1414	1542	.	+	0	ID=BBa_S05347;Name=BBa_S05347
BBa_K1896012	.	terminator	1414	1493	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S05347
BBa_K1896012	.	engineered_region	1414	1493	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1896012	.	stem_loop	1425	1468	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1896012	.	terminator	1502	1542	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S05347
BBa_K1896012	.	stop_codon	1535	1535	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1896012	.	polyA_site	1529	1542	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1896012	.	stem_loop	1509	1528	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1896012	.	engineered_region	1502	1542	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1896012	.	engineered_region	1	55	.	+	0	ID=BBa_S05357;Name=BBa_S05357
BBa_K1896012	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_S05357
BBa_K1896012	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05357
BBa_K1896012	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1896012	.	engineered_region	62	1405	.	+	0	ID=BBa_S05358;Name=BBa_S05358
BBa_K1896012	.	CDS	62	985	.	+	0	ID=BBa_K1896003;Name=BBa_K1896003;Parent=BBa_S05358
BBa_K1896012	.	point_mutation	721	721	.	+	0	ID=annotation2495421;Name=BsaI site removed;Parent=BBa_K1896003
BBa_K1896012	.	start_codon	62	64	.	+	0	ID=annotation2495419;Name=start;Parent=BBa_K1896003
BBa_K1896012	.	CDS	62	985	.	+	0	ID=annotation2495420;Name=INP_NC;Parent=BBa_K1896003
BBa_K1896012	.	CDS	986	1405	.	+	0	ID=BBa_K1896004;Name=BBa_K1896004;Parent=BBa_S05358
BBa_K1896012	.	CDS	1382	1399	.	+	0	ID=annotation2497044;Name=His-tag;Parent=BBa_K1896004
BBa_K1896012	.	stop_codon	1400	1405	.	+	0	ID=annotation2497045;Name=stop;Parent=BBa_K1896004
BBa_K1896012	.	point_mutation	1045	1045	.	+	0	ID=annotation2497041;Name=BamHI site removed;Parent=BBa_K1896004
BBa_K1896012	.	CDS	986	1000	.	+	0	ID=annotation2497040;Name=GSTGS linker;Parent=BBa_K1896004
BBa_K1896012	.	CDS	1001	1381	.	+	0	ID=annotation2497042;Name=Streptavidin;Parent=BBa_K1896004
>BBa_K1896012
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgaccctggataaagcg
ctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagcc
gctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgag
cgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtg
aaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatc
cagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgt
agcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgct
cctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataacc
attctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtac
gctgatggccggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatc
gggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtc
agctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtgga
caaaccggacgaagatgatgattggatcgaagtgaaaggcagcaccggatctgctgaagctggtatcaccggcacctgg
tacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttg
gtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctggg
ttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgct
gaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtc
acgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata