LOCUS       BBa_K1896013            1518 bp    DNA     linear       22-Nov-2024
DEFINITION  INP_NC-mSA2 generator
ACCESSION   BBa_K1896013
VERSION     BBa_K1896013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S05357
     misc_feature    62..64
                     /label=start
     CDS             62..985
                     /label=INP_NC
     misc_feature    721..721
                     /label=BsaI site removed
     CDS             62..985
                     /label=BBa_K1896003
     CDS             986..1000
                     /label=GSTGS linker
     CDS             1001..1357
                     /label=mSA2
     CDS             1358..1375
                     /label=His-tag
     misc_feature    1376..1381
                     /label=stop
     CDS             986..1381
                     /label=BBa_K1896005
     misc_feature    62..1381
                     /label=BBa_S05359
     stem_loop       1401..1444
                     /label=stem_loop
     misc_feature    1390..1469
                     /label=BBa_B0010
     terminator      1390..1469
                     /label=BBa_B0010
     stem_loop       1485..1504
                     /label=T7 TE
     misc_feature    1511..1511
                     /label=stop
     polyA_site      1505..1518
                     /label=polya
     misc_feature    1478..1518
                     /label=BBa_B0012
     terminator      1478..1518
                     /label=BBa_B0012
     misc_feature    1390..1518
                     /label=BBa_S05347
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatgaccctg gataaagcgc tggtgctgcg cacttgtgcc aataatatgg ccgaccattg
      121 tggcctgatc tggcctgctt ctggtacagt tgaaagccgc tattggcagt ctacacgtcg
      181 tcacgaaaac gggctggttg ggctgctgtg gggtgccgga acatccgcct ttctgagcgt
      241 tcatgccgat gctcgttgga ttgtttgtga ggttgctgtt gccgacatca ttagcctgga
      301 agaaccgggt atggtgaaat ttcctcgtgc tgaagtggtc catgttggtg atcgtattag
      361 cgcctcccat ttcatttcag ctcgtcaggc tgatccagca agcacatcaa caagcacctc
      421 taccagtaca ctgacaccaa tgccaaccgc tatccctact cctatgccgg ctgtagcctc
      481 tgttactctg cctgtggccg aacaagctcg ccacgaagtc tttgacgttg cctctgtgtc
      541 tgctgctgct gctcctgtta acactctgcc tgttaccacg cctcaaaatc tgcaaacggc
      601 gacctatggc tcaacactga gtggtgataa ccattctcgt ctgattgccg ggtatggctc
      661 taacgaaacc gccggaaacc attctgatct gatcggtggt cacgattgta cgctgatggc
      721 cggtgatcaa tctcgtctga ccgctggcaa aaattccgtt ctgaccgctg gggcacgttc
      781 taaactgatc gggagcgaag gctctactct gtccgccgga gaagattcta ccctgatctt
      841 tcgtctgtgg gatggtaaac gttatcgtca gctggtcgct cgtacaggtg aaaatggtgt
      901 ggaagccgac attccgtatt atgtgaatga ggacgatgat attgtggaca aaccggacga
      961 agatgatgat tggatcgaag tgaaaggcag caccggatct gcggaagcgg gtatcaccgg
     1021 cacgtggtac aaccagcatg gttctacctt caccgttacc gcgggtgcgg acggtaacct
     1081 gaccggtcag tacgaaaacc gtgcgcaggg cactggttgc cagaactctc cgtacaccct
     1141 gaccggtcgt tacaacggta ccaaactgga atggcgtgtt gaatggaaca actctaccga
     1201 aaactgccac tctcgtaccg aatggcgtgg tcagtaccag ggtggtgcgg aagcgcgtat
     1261 caacacccag tggaacctga cctacgaagg tggttctggt ccggcgaccg aacagggtca
     1321 ggacaccttc accaaagtta aaccgtctgc ggcgtcccat caccatcacc accattaata
     1381 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1441 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1501 ggcctttctg cgtttata
//