LOCUS       BBa_K1896014            1332 bp    DNA     linear       25-Nov-2024
DEFINITION  mGFPuv2-streptavidin generator
ACCESSION   BBa_K1896014
VERSION     BBa_K1896014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S05355
     CDS             62..775
                     /label=mGFPuv2
     misc_feature    356..358
                     /label=F99S
     misc_feature    518..520
                     /label=M153T
     misc_feature    548..550
                     /label=V163A
     misc_feature    677..679
                     /label=A206K
     misc_feature    683..685
                     /label=S208L
     misc_feature    62..64
                     /label=start
     CDS             62..775
                     /label=BBa_K1896006
     CDS             776..790
                     /label=GSTGS linker
     misc_feature    835..835
                     /label=BamHI site removed
     CDS             791..1171
                     /label=Streptavidin
     CDS             1172..1189
                     /label=His-tag
     misc_feature    1190..1195
                     /label=stop
     CDS             776..1195
                     /label=BBa_K1896004
     misc_feature    62..1195
                     /label=BBa_S05360
     stem_loop       1215..1258
                     /label=stem_loop
     misc_feature    1204..1283
                     /label=BBa_B0010
     terminator      1204..1283
                     /label=BBa_B0010
     stem_loop       1299..1318
                     /label=T7 TE
     misc_feature    1325..1325
                     /label=stop
     polyA_site      1319..1332
                     /label=polya
     misc_feature    1292..1332
                     /label=BBa_B0012
     terminator      1292..1332
                     /label=BBa_B0012
     misc_feature    1204..1332
                     /label=BBa_S05347
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      361 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      481 actggagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      541 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggctccgttc aactagcaga
      601 ccattatcaa caaaatactc caattggtga tggccctgtc cttttaccag acaaccatta
      661 cctgtcgaca caatctaaac ttttgaaaga tcccaacgaa aagcgtgacc acatggtcct
      721 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaaggcag
      781 caccggatct gctgaagctg gtatcaccgg cacctggtac aaccagctgg gatctacctt
      841 catcgttacc gctggtgctg acggtgctct gaccggtacc tacgaatccg ctgttggtaa
      901 cgctgaaagc cgctacgttc tgaccggtcg ttacgactcc gctccggcta ccgacggttc
      961 cggaaccgct ctgggttgga ccgttgcttg gaaaaacaac taccgtaacg ctcactccgc
     1021 taccacctgg tctggccagt acgttggtgg tgctgaagct cgtatcaaca cccagtggtt
     1081 gttgacctcc ggcaccaccg aagccaacgc gtggaaatcc accctggttg gtcacgacac
     1141 cttcaccaaa gttaaaccgt ccgctgcttc ccatcaccat caccaccatt aataatacta
     1201 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1261 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1321 tctgcgttta ta
//