LOCUS       BBa_K1896015            1308 bp    DNA     linear       30-Jun-2024
DEFINITION  mGFPuv2-mSA2 generator
ACCESSION   BBa_K1896015
VERSION     BBa_K1896015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S05355
     CDS             62..775
                     /label=mGFPuv2
     misc_feature    356..358
                     /label=F99S
     misc_feature    518..520
                     /label=M153T
     misc_feature    548..550
                     /label=V163A
     misc_feature    677..679
                     /label=A206K
     misc_feature    683..685
                     /label=S208L
     misc_feature    62..64
                     /label=start
     CDS             62..775
                     /label=BBa_K1896006
     CDS             776..790
                     /label=GSTGS linker
     CDS             791..1147
                     /label=mSA2
     CDS             1148..1165
                     /label=His-tag
     misc_feature    1166..1171
                     /label=stop
     CDS             776..1171
                     /label=BBa_K1896005
     misc_feature    62..1171
                     /label=BBa_S05361
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     misc_feature    1180..1308
                     /label=BBa_S05347
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      361 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      481 actggagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      541 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggctccgttc aactagcaga
      601 ccattatcaa caaaatactc caattggtga tggccctgtc cttttaccag acaaccatta
      661 cctgtcgaca caatctaaac ttttgaaaga tcccaacgaa aagcgtgacc acatggtcct
      721 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaaggcag
      781 caccggatct gcggaagcgg gtatcaccgg cacgtggtac aaccagcatg gttctacctt
      841 caccgttacc gcgggtgcgg acggtaacct gaccggtcag tacgaaaacc gtgcgcaggg
      901 cactggttgc cagaactctc cgtacaccct gaccggtcgt tacaacggta ccaaactgga
      961 atggcgtgtt gaatggaaca actctaccga aaactgccac tctcgtaccg aatggcgtgg
     1021 tcagtaccag ggtggtgcgg aagcgcgtat caacacccag tggaacctga cctacgaagg
     1081 tggttctggt ccggcgaccg aacagggtca ggacaccttc accaaagtta aaccgtctgc
     1141 ggcgtcccat caccatcacc accattaata atactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//