##gff-version 3
##sequence-region BBa_K1896015 1 1308
BBa_K1896015	.	engineered_region	1180	1308	.	+	0	ID=BBa_S05347;Name=BBa_S05347
BBa_K1896015	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S05347
BBa_K1896015	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1896015	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1896015	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S05347
BBa_K1896015	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1896015	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1896015	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1896015	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1896015	.	engineered_region	62	1171	.	+	0	ID=BBa_S05361;Name=BBa_S05361
BBa_K1896015	.	CDS	776	1171	.	+	0	ID=BBa_K1896005;Name=BBa_K1896005;Parent=BBa_S05361
BBa_K1896015	.	CDS	791	1147	.	+	0	ID=annotation2499131;Name=mSA2;Parent=BBa_K1896005
BBa_K1896015	.	CDS	1148	1165	.	+	0	ID=annotation2499132;Name=His-tag;Parent=BBa_K1896005
BBa_K1896015	.	CDS	776	790	.	+	0	ID=annotation2499130;Name=GSTGS linker;Parent=BBa_K1896005
BBa_K1896015	.	stop_codon	1166	1171	.	+	0	ID=annotation2499133;Name=stop;Parent=BBa_K1896005
BBa_K1896015	.	CDS	62	775	.	+	0	ID=BBa_K1896006;Name=BBa_K1896006;Parent=BBa_S05361
BBa_K1896015	.	sequence_alteration	548	550	.	+	0	ID=annotation2499182;Name=V163A;Parent=BBa_K1896006
BBa_K1896015	.	sequence_alteration	518	520	.	+	0	ID=annotation2499181;Name=M153T;Parent=BBa_K1896006
BBa_K1896015	.	CDS	62	775	.	+	0	ID=annotation2499179;Name=mGFPuv2;Parent=BBa_K1896006
BBa_K1896015	.	sequence_alteration	683	685	.	+	0	ID=annotation2499184;Name=S208L;Parent=BBa_K1896006
BBa_K1896015	.	sequence_alteration	356	358	.	+	0	ID=annotation2499180;Name=F99S;Parent=BBa_K1896006
BBa_K1896015	.	start_codon	62	64	.	+	0	ID=annotation2499187;Name=start;Parent=BBa_K1896006
BBa_K1896015	.	sequence_alteration	677	679	.	+	0	ID=annotation2499183;Name=A206K;Parent=BBa_K1896006
BBa_K1896015	.	engineered_region	1	55	.	+	0	ID=BBa_S05355;Name=BBa_S05355
BBa_K1896015	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_S05355
BBa_K1896015	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05355
BBa_K1896015	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1896015
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaag
tcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcgg
acacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaac
ttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatg
gccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgacca
catggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatct
gcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacc
tgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacgg
taccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtac
cagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagg
gtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata