BBa_K1896015
type: ComponentDefinition
persistent identity: BBa K1896015
display id: BBa_K1896015
version: 1
was generated by: iGEM to SBOL conversion
description: mGFPuv2-mSA2 generator
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T05:27:21Z
mutable provenance: todo
owned by: james
owned by: myers
mutable description: todo
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: todo
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_S05347
component: BBa_S05361
component: BBa_S05355
sequence annotation: BBa_S05355
sequence annotation: BBa_S05361
sequence annotation: BBa_S05347
sequence: https://synbiohub.org/public/igem/BBa_K1896015_sequence/1

BBa_S05347
type: Component
persistent identity: component2500029
display id: component2500029
version: 1
top level: BBa_K1896015
definition: BBa_S05347
access: public

BBa_S05361
type: Component
persistent identity: component2500022
display id: component2500022
version: 1
top level: BBa_K1896015
definition: BBa_S05361
access: public

BBa_S05355
type: Component
persistent identity: component2500008
display id: component2500008
version: 1
top level: BBa_K1896015
definition: BBa_S05355
access: public

BBa_S05355
type: SequenceAnnotation
persistent identity: annotation2500008
display id: annotation2500008
version: 1
top level: BBa_K1896015
location: https://synbiohub.org/public/igem/BBa_K1896015/annotation2500008/range2500008/1
component: BBa_S05355

https://synbiohub.org/public/igem/BBa_K1896015/annotation2500008/range2500008/1
type: Range
persistent identity: range2500008
display id: range2500008
version: 1
top level: BBa_K1896015
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_S05361
type: SequenceAnnotation
persistent identity: annotation2500022
display id: annotation2500022
version: 1
top level: BBa_K1896015
location: https://synbiohub.org/public/igem/BBa_K1896015/annotation2500022/range2500022/1
component: BBa_S05361

https://synbiohub.org/public/igem/BBa_K1896015/annotation2500022/range2500022/1
type: Range
persistent identity: range2500022
display id: range2500022
version: 1
top level: BBa_K1896015
direction: direction/reverse
start: 62
end: 1171
orientation: inline

BBa_S05347
type: SequenceAnnotation
persistent identity: annotation2500029
display id: annotation2500029
version: 1
top level: BBa_K1896015
location: https://synbiohub.org/public/igem/BBa_K1896015/annotation2500029/range2500029/1
component: BBa_S05347

https://synbiohub.org/public/igem/BBa_K1896015/annotation2500029/range2500029/1
type: Range
persistent identity: range2500029
display id: range2500029
version: 1
top level: BBa_K1896015
direction: direction/reverse
start: 1180
end: 1308
orientation: inline

BBa_S05347
type: ComponentDefinition
persistent identity: BBa S05347
display id: BBa_S05347
version: 1
was generated by: iGEM to SBOL conversion
description: B0010:B0012
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T02:32:06Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S05347_sequence/1

BBa_B0012
type: Component
persistent identity: component2495234
display id: component2495234
version: 1
top level: BBa_S05347
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2495232
display id: component2495232
version: 1
top level: BBa_S05347
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2495232
display id: annotation2495232
version: 1
top level: BBa_S05347
location: https://synbiohub.org/public/igem/BBa_S05347/annotation2495232/range2495232/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S05347/annotation2495232/range2495232/1
type: Range
persistent identity: range2495232
display id: range2495232
version: 1
top level: BBa_S05347
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2495234
display id: annotation2495234
version: 1
top level: BBa_S05347
location: https://synbiohub.org/public/igem/BBa_S05347/annotation2495234/range2495234/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S05347/annotation2495234/range2495234/1
type: Range
persistent identity: range2495234
display id: range2495234
version: 1
top level: BBa_S05347
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S05355
type: ComponentDefinition
persistent identity: BBa S05355
display id: BBa_S05355
version: 1
was generated by: iGEM to SBOL conversion
description: J23119:B0034
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T12:33:03Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05355_sequence/1

BBa_B0034
type: Component
persistent identity: component2499223
display id: component2499223
version: 1
top level: BBa_S05355
definition: BBa_B0034
access: public

BBa_J23119
type: Component
persistent identity: component2499221
display id: component2499221
version: 1
top level: BBa_S05355
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2499221
display id: annotation2499221
version: 1
top level: BBa_S05355
location: https://synbiohub.org/public/igem/BBa_S05355/annotation2499221/range2499221/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_S05355/annotation2499221/range2499221/1
type: Range
persistent identity: range2499221
display id: range2499221
version: 1
top level: BBa_S05355
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2499223
display id: annotation2499223
version: 1
top level: BBa_S05355
location: https://synbiohub.org/public/igem/BBa_S05355/annotation2499223/range2499223/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05355/annotation2499223/range2499223/1
type: Range
persistent identity: range2499223
display id: range2499223
version: 1
top level: BBa_S05355
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_S05361
type: ComponentDefinition
persistent identity: BBa S05361
display id: BBa_S05361
version: 1
was generated by: iGEM to SBOL conversion
description: K1896006:K1896005
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T05:48:33Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1896005
component: BBa_K1896006
sequence annotation: BBa_K1896006
sequence annotation: BBa_K1896005
sequence: https://synbiohub.org/public/igem/BBa_S05361_sequence/1

BBa_K1896005
type: Component
persistent identity: component2499845
display id: component2499845
version: 1
top level: BBa_S05361
definition: BBa_K1896005
access: public

BBa_K1896006
type: Component
persistent identity: component2499840
display id: component2499840
version: 1
top level: BBa_S05361
definition: BBa_K1896006
access: public

BBa_K1896006
type: SequenceAnnotation
persistent identity: annotation2499840
display id: annotation2499840
version: 1
top level: BBa_S05361
location: https://synbiohub.org/public/igem/BBa_S05361/annotation2499840/range2499840/1
component: BBa_K1896006

https://synbiohub.org/public/igem/BBa_S05361/annotation2499840/range2499840/1
type: Range
persistent identity: range2499840
display id: range2499840
version: 1
top level: BBa_S05361
direction: direction/reverse
start: 1
end: 714
orientation: inline

BBa_K1896005
type: SequenceAnnotation
persistent identity: annotation2499845
display id: annotation2499845
version: 1
top level: BBa_S05361
location: https://synbiohub.org/public/igem/BBa_S05361/annotation2499845/range2499845/1
component: BBa_K1896005

https://synbiohub.org/public/igem/BBa_S05361/annotation2499845/range2499845/1
type: Range
persistent identity: range2499845
display id: range2499845
version: 1
top level: BBa_S05361
direction: direction/reverse
start: 715
end: 1110
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K1896005
type: ComponentDefinition
persistent identity: BBa K1896005
display id: BBa_K1896005
version: 1
was generated by: iGEM to SBOL conversion
description: mSA2-His
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T05:41:52Z
mutable provenance: Combination of streptavidin (''Streptomyces avidinii'') and rhizavidin (''Rhizobium etli''), engineered through homology modeling [1].
owned by: james
owned by: myers
mutable description: Variant of Part:BBa_K1896000 for use in scarless C-terminal protein fusions. This part contains a His-tag instead of a terminal AgeI restriction site.
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part is not compatible with the Freiburg assembly standard (RFC 25).
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GSTGS linker
sequence annotation: mSA2
sequence annotation: His-tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1896005_sequence/1

GSTGS linker
type: SequenceAnnotation
persistent identity: annotation2499130
display id: annotation2499130
version: 1
top level: BBa_K1896005
location: https://synbiohub.org/public/igem/BBa_K1896005/annotation2499130/range2499130/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896005/annotation2499130/range2499130/1
type: Range
persistent identity: range2499130
display id: range2499130
version: 1
top level: BBa_K1896005
direction: direction/forward
start: 1
end: 15
orientation: inline

mSA2
type: SequenceAnnotation
persistent identity: annotation2499131
display id: annotation2499131
version: 1
top level: BBa_K1896005
location: https://synbiohub.org/public/igem/BBa_K1896005/annotation2499131/range2499131/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896005/annotation2499131/range2499131/1
type: Range
persistent identity: range2499131
display id: range2499131
version: 1
top level: BBa_K1896005
direction: direction/forward
start: 16
end: 372
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2499132
display id: annotation2499132
version: 1
top level: BBa_K1896005
location: https://synbiohub.org/public/igem/BBa_K1896005/annotation2499132/range2499132/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896005/annotation2499132/range2499132/1
type: Range
persistent identity: range2499132
display id: range2499132
version: 1
top level: BBa_K1896005
direction: direction/forward
start: 373
end: 390
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2499133
display id: annotation2499133
version: 1
top level: BBa_K1896005
location: https://synbiohub.org/public/igem/BBa_K1896005/annotation2499133/range2499133/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1896005/annotation2499133/range2499133/1
type: Range
persistent identity: range2499133
display id: range2499133
version: 1
top level: BBa_K1896005
direction: direction/forward
start: 391
end: 396
orientation: inline

BBa_K1896006
type: ComponentDefinition
persistent identity: BBa K1896006
display id: BBa_K1896006
version: 1
was generated by: iGEM to SBOL conversion
description: mGFPuv2 (N-part)
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T05:42:05Z
mutable provenance: We started from pBAD-GFPuv (GenBank: U62637.1, Clontech), applied the described mutations and codon optimised the sequence for ''E. coli''.
owned by: james
owned by: myers
mutable description: Variant of Part:BBa_K1896001 that lacks stop codons so it can be used in N-terminal protein fusions. mGFPuv2 contains the following mutations: * '''F99S/M153T/V163A''': GFPuv or ''cycle 3'' GFP was optimised for excitation by UV light (360-400nm). [1] * '''S208L''': Almost doubles the fluorescent intensity of GFPuv, resulting in GFPuv2. [2] * '''A206K''': Monomerizes most GFP variants by disrupting a hydrophobic patch. [3]
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon was removed to enable scarless N-terminal protein fusions.
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mGFPuv2
sequence annotation: start
sequence annotation: F99S
sequence annotation: M153T
sequence annotation: V163A
sequence annotation: A206K
sequence annotation: S208L
sequence: https://synbiohub.org/public/igem/BBa_K1896006_sequence/1

mGFPuv2
type: SequenceAnnotation
persistent identity: annotation2499179
display id: annotation2499179
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499179/range2499179/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499179/range2499179/1
type: Range
persistent identity: range2499179
display id: range2499179
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 1
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2499187
display id: annotation2499187
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499187/range2499187/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499187/range2499187/1
type: Range
persistent identity: range2499187
display id: range2499187
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 1
end: 3
orientation: inline

F99S
type: SequenceAnnotation
persistent identity: annotation2499180
display id: annotation2499180
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499180/range2499180/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499180/range2499180/1
type: Range
persistent identity: range2499180
display id: range2499180
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 295
end: 297
orientation: inline

M153T
type: SequenceAnnotation
persistent identity: annotation2499181
display id: annotation2499181
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499181/range2499181/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499181/range2499181/1
type: Range
persistent identity: range2499181
display id: range2499181
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 457
end: 459
orientation: inline

V163A
type: SequenceAnnotation
persistent identity: annotation2499182
display id: annotation2499182
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499182/range2499182/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499182/range2499182/1
type: Range
persistent identity: range2499182
display id: range2499182
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 487
end: 489
orientation: inline

A206K
type: SequenceAnnotation
persistent identity: annotation2499183
display id: annotation2499183
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499183/range2499183/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499183/range2499183/1
type: Range
persistent identity: range2499183
display id: range2499183
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 616
end: 618
orientation: inline

S208L
type: SequenceAnnotation
persistent identity: annotation2499184
display id: annotation2499184
version: 1
top level: BBa_K1896006
location: https://synbiohub.org/public/igem/BBa_K1896006/annotation2499184/range2499184/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1896006/annotation2499184/range2499184/1
type: Range
persistent identity: range2499184
display id: range2499184
version: 1
top level: BBa_K1896006
direction: direction/forward
start: 622
end: 624
orientation: inline

https://synbiohub.org/public/igem/BBa_K1896015_sequence/1
type: Sequence
persistent identity: BBa K1896015 sequence
display id: BBa_K1896015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05347_sequence/1
type: Sequence
persistent identity: BBa S05347 sequence
display id: BBa_S05347_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05355_sequence/1
type: Sequence
persistent identity: BBa S05355 sequence
display id: BBa_S05355_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05361_sequence/1
type: Sequence
persistent identity: BBa S05361 sequence
display id: BBa_S05361_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaaggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1896005_sequence/1
type: Sequence
persistent identity: BBa K1896005 sequence
display id: BBa_K1896005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcagcaccggatctgcggaagcgggtatcaccggcacgtggtacaaccagcatggttctaccttcaccgttaccgcgggtgcggacggtaacctgaccggtcagtacgaaaaccgtgcgcagggcactggttgccagaactctccgtacaccctgaccggtcgttacaacggtaccaaactggaatggcgtgttgaatggaacaactctaccgaaaactgccactctcgtaccgaatggcgtggtcagtaccagggtggtgcggaagcgcgtatcaacacccagtggaacctgacctacgaaggtggttctggtccggcgaccgaacagggtcaggacaccttcaccaaagttaaaccgtctgcggcgtcccatcaccatcaccaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1896006_sequence/1
type: Sequence
persistent identity: BBa K1896006 sequence
display id: BBa_K1896006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactggagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggctccgttcaactagcagaccattatcaacaaaatactccaattggtgatggccctgtccttttaccagacaaccattacctgtcgacacaatctaaacttttgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z