LOCUS BBa_K1896017 3714 bp DNA linear 25-Nov-2024 DEFINITION INP_RC-streptavidin generator ACCESSION BBa_K1896017 VERSION BBa_K1896017.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23105 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1..55 /label=BBa_S05357 CDS 62..3157 /label=inaZ_RC misc_feature 62..64 /label=start CDS 83..3010 /label=repeat region CDS 62..3157 /label=BBa_K1896008 CDS 3158..3172 /label=GSTGS linker misc_feature 3217..3217 /label=BamHI site removed CDS 3173..3553 /label=Streptavidin CDS 3554..3571 /label=His-tag misc_feature 3572..3577 /label=stop CDS 3158..3577 /label=BBa_K1896004 misc_feature 62..3577 /label=BBa_S05363 stem_loop 3597..3640 /label=stem_loop misc_feature 3586..3665 /label=BBa_B0010 terminator 3586..3665 /label=BBa_B0010 stem_loop 3681..3700 /label=T7 TE misc_feature 3707..3707 /label=stop polyA_site 3701..3714 /label=polya misc_feature 3674..3714 /label=BBa_B0012 terminator 3674..3714 /label=BBa_B0012 misc_feature 3586..3714 /label=BBa_S05347 ORIGIN 1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta 61 gatgccgcag aatgtgcaga ccgccactta cggcagcacg ttgagtggcg acaatcacag 121 tcgtctgatt gccggttatg gcagtaacga aaccgctggc aaccacagtg atctaattgc 181 cggttatgga agtacaggca ccgccggctc cgacagctgg ctggtcgctg gctatggaag 241 cacccagacc gccggtgggg acagcgcgct gacagcgggt tacggcagca cccagaccgc 301 ccgcgaaggc agcaacctga cggcagggta cggcagcacc ggcacggcag gctcggacag 361 ttcgctgatc gccggttacg gcagtactca gacttcgggc ggggacagct cactcacagc 421 gggttacggc agcacgcaaa cggctcagga aggcagcaat ctcaccgctg ggtatggcag 481 caccggcacg gcaggctcgg acagctcctt gatcgccggt tatggcagta cacaaacctc 541 gggaggcgac agttcgctga ccgcgggcta cggcagtacg cagacggccc aggagggcag 601 caatctgacg gcggggtacg gcagcacggg tacagcaggt gtcgacagct ctctgatcgc 661 gggatacggc agcacgcaga cctcgggaag tgacagcgcc ctgaccgcag gctatggcag 721 cacgcaaacg gcccaggaag gcagcaatct cactgctggg tatggcagca ccggcacggc 781 aggttccgac agctcgctga tcgccggtta cggcagcacg caaacctcgg gcagtgacag 841 ctcgctcacg gcggggtacg gcagtacgca gacggctcag gaaggcagca ttctgacggc 901 ggggtacggc agcacgggta cagcaggtgt cgacagttcg ttgatcgccg gatatggcag 961 cacgcagacc tcgggaagtg acagtgcgct gacagcgggt tacggcagca cgcaaacggc 1021 ccaggaaggc agcaacctga cggcgggcta cggcagcact ggcacggcag gtgccgacag 1081 ttcgttgatc gccggatatg gcagcacgca gacgtcaggc agcgaaagtt cgcttaccgc 1141 aggctatggc agtacccaga ctgcccgtga gggcagcacc ctgacggccg gatatggcag 1201 taccggaaca gctggcgctg acagctcgct gatcgccggt tacggcagca cgcaaacctc 1261 gggcagtgaa agctcgctca cggcaggtta tggcagtacc cagaccgcac agcagggcag 1321 cgtactcaca tcaggctatg gcagtacgca aacggccggg gctgccagta acctcaccac 1381 cggttacgga agtacaggta ccgcaggtca cgagagtttc atcattgcgg gttatggaag 1441 tacacagaca gcgggccaca aaagtatcct gaccgctggt tatggcagta ctcagacggc 1501 cagggacggt agcgacctga ttgcgggcta tggcagtacg ggaaccgcag gctcgggcag 1561 ttcgctgatc gcaggttatg gcagcaccca gaccgcgagt tacagaagca tgctgaccgc 1621 cggttatggc agtacccaga ccgccagaga acacagcgac cttgtcacag gctatggcag 1681 cacttcaacg gcagggtcaa acagttcgct gatcgccggc tatggaagca ctcagacggc 1741 gggcttcaaa agcatactga ccgccggtta cggcagtacc cagacggcac aggagcgcac 1801 gagcctggtc gcaggctacg gaagcacgtc gactgcgggc tattccagtt ccttgatcgc 1861 cggctatggc agcacgcaga cggcaggcta cgaaagcacg ttgaccgccg gttacggcag 1921 tacgcaaacc gctcaggaaa acagctcgct caccacaggt tacggaagta cttctactgc 1981 gggctattcc agctcgctca tcgcgggtta cggcagtacg caaacggcag gctacgagag 2041 cacgttgacc gccggttacg gtagtacgca aaccgcgcag gagcgcagtg atctggtgac 2101 aggttatgga agtacctcca ccgccggcta tgcgagctcg ctgattgcgg gttatggcag 2161 cacgcagact gcgggttatg agagcacgtt gaccgccggt tacggcagca cgcaaaccgc 2221 acaggaaaac agctcgctca ccaccgggta cggaagtacc tccacagccg gctttgccag 2281 ctcgctgatc tccggttatg gcagtacgca gacagccggc tataaaagta ccctcacggc 2341 cggttacggc agtactcaga ccgcagagta tggaagctca ctcactgcgg gctacggcag 2401 cactgcaacg gccgggcagg acagttcatt gatagccggc tatggcagct ccctgaccag 2461 cggaatcaga agttttctga cggcaggcta tggcagtacg ctgatcgccg gacttcgcag 2521 cgttttgatc gccggttatg gcagtagtct tacatcgggc gttcgcagca cgttgactgc 2581 gggttatggc agtaaccaga ttgcaagtta cggcagctcg ttgattgcag gccatgaaag 2641 cattcaggtc gccggaaata aaagcatgct gatcgccggc aagggcagct cgcagacagc 2701 aggttttcgc agcacgctga ttgccggtgc gggcagtgta caactggcgg gtgatcgcag 2761 ccggttgatt gccggtgcag acagtaatca gaccgcgggt gaccgcagca aactgctggc 2821 cggtaataac agttatctga ctgccggcga tagaagcaaa ctgaccggcg ggcatgactg 2881 caccctgatg gcgggtgacc aaagcagatt gaccgctggt aagaacagtg tcttgacggc 2941 aggcgctcgt agcaaactga ttggcagtga aggctcgacg ctctcggctg gagaagactc 3001 catacttatt ttcaggctct gggacgggaa gaggtacagg caactggtcg ccagaacggg 3061 tgagaacggt gttgaggccg acataccgta ttacgtgaac gaagatgacg atattgtcga 3121 taaacccgac gaggacgatg actggataga ggtaaagggc agcaccggat ctgctgaagc 3181 tggtatcacc ggcacctggt acaaccagct gggatctacc ttcatcgtta ccgctggtgc 3241 tgacggtgct ctgaccggta cctacgaatc cgctgttggt aacgctgaaa gccgctacgt 3301 tctgaccggt cgttacgact ccgctccggc taccgacggt tccggaaccg ctctgggttg 3361 gaccgttgct tggaaaaaca actaccgtaa cgctcactcc gctaccacct ggtctggcca 3421 gtacgttggt ggtgctgaag ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac 3481 cgaagccaac gcgtggaaat ccaccctggt tggtcacgac accttcacca aagttaaacc 3541 gtccgctgct tcccatcacc atcaccacca ttaataatac tagagccagg catcaaataa 3601 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 3661 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //