##gff-version 3
##sequence-region BBa_K1896017 1 3714
BBa_K1896017	.	engineered_region	1	55	.	+	0	ID=BBa_S05357;Name=BBa_S05357
BBa_K1896017	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_S05357
BBa_K1896017	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S05357
BBa_K1896017	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1896017	.	engineered_region	62	3577	.	+	0	ID=BBa_S05363;Name=BBa_S05363
BBa_K1896017	.	CDS	3158	3577	.	+	0	ID=BBa_K1896004;Name=BBa_K1896004;Parent=BBa_S05363
BBa_K1896017	.	CDS	3173	3553	.	+	0	ID=annotation2497042;Name=Streptavidin;Parent=BBa_K1896004
BBa_K1896017	.	point_mutation	3217	3217	.	+	0	ID=annotation2497041;Name=BamHI site removed;Parent=BBa_K1896004
BBa_K1896017	.	CDS	3158	3172	.	+	0	ID=annotation2497040;Name=GSTGS linker;Parent=BBa_K1896004
BBa_K1896017	.	stop_codon	3572	3577	.	+	0	ID=annotation2497045;Name=stop;Parent=BBa_K1896004
BBa_K1896017	.	CDS	3554	3571	.	+	0	ID=annotation2497044;Name=His-tag;Parent=BBa_K1896004
BBa_K1896017	.	CDS	62	3157	.	+	0	ID=BBa_K1896008;Name=BBa_K1896008;Parent=BBa_S05363
BBa_K1896017	.	start_codon	62	64	.	+	0	ID=annotation2502231;Name=start;Parent=BBa_K1896008
BBa_K1896017	.	CDS	62	3157	.	+	0	ID=annotation2502230;Name=inaZ_RC;Parent=BBa_K1896008
BBa_K1896017	.	CDS	83	3010	.	+	0	ID=annotation2502248;Name=repeat region;Parent=BBa_K1896008
BBa_K1896017	.	engineered_region	3586	3714	.	+	0	ID=BBa_S05347;Name=BBa_S05347
BBa_K1896017	.	terminator	3586	3665	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S05347
BBa_K1896017	.	stem_loop	3597	3640	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1896017	.	engineered_region	3586	3665	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1896017	.	terminator	3674	3714	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S05347
BBa_K1896017	.	stem_loop	3681	3700	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1896017	.	polyA_site	3701	3714	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1896017	.	stop_codon	3707	3707	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1896017	.	engineered_region	3674	3714	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1896017
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgccgcagaatgtgcag
accgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctg
gcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatgg
aagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaac
ctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagactt
cgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggta
tggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagt
tcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggta
cagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcagg
ctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgac
agctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgc
agacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgc
cggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaa
ggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagca
cgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgac
ggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggc
agtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggca
gtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcat
cattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggcc
agggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatg
gcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcga
ccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacg
gcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggct
acggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaag
cacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttct
actgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccg
gttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgc
gagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacg
caaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatct
ccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagta
tggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagc
tccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttga
tcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaag
ttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggc
agctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggt
tgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgc
cggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaag
aacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactcca
tacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccga
cataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaagggc
agcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtg
ctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacga
ctccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcac
tccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggca
ccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttccca
tcaccatcaccaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a