BBa_K1896017
type: ComponentDefinition
persistent identity: BBa K1896017
display id: BBa_K1896017
version: 1
was generated by: iGEM to SBOL conversion
description: INP_RC-streptavidin generator
created: 2016-10-12T11:00:00Z
modified: 2016-10-18T01:43:24Z
mutable provenance: todo
owned by: james
owned by: myers
mutable description: todo
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: todo
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_S05347
component: BBa_S05363
component: BBa_S05357
sequence annotation: BBa_S05357
sequence annotation: BBa_S05363
sequence annotation: BBa_S05347
sequence: https://synbiohub.org/public/igem/BBa_K1896017_sequence/1

BBa_S05347
type: Component
persistent identity: component2500062
display id: component2500062
version: 1
top level: BBa_K1896017
definition: BBa_S05347
access: public

BBa_S05363
type: Component
persistent identity: component2500055
display id: component2500055
version: 1
top level: BBa_K1896017
definition: BBa_S05363
access: public

BBa_S05357
type: Component
persistent identity: component2500047
display id: component2500047
version: 1
top level: BBa_K1896017
definition: BBa_S05357
access: public

BBa_S05357
type: SequenceAnnotation
persistent identity: annotation2500047
display id: annotation2500047
version: 1
top level: BBa_K1896017
location: https://synbiohub.org/public/igem/BBa_K1896017/annotation2500047/range2500047/1
component: BBa_S05357

https://synbiohub.org/public/igem/BBa_K1896017/annotation2500047/range2500047/1
type: Range
persistent identity: range2500047
display id: range2500047
version: 1
top level: BBa_K1896017
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_S05363
type: SequenceAnnotation
persistent identity: annotation2500055
display id: annotation2500055
version: 1
top level: BBa_K1896017
location: https://synbiohub.org/public/igem/BBa_K1896017/annotation2500055/range2500055/1
component: BBa_S05363

https://synbiohub.org/public/igem/BBa_K1896017/annotation2500055/range2500055/1
type: Range
persistent identity: range2500055
display id: range2500055
version: 1
top level: BBa_K1896017
direction: direction/reverse
start: 62
end: 3577
orientation: inline

BBa_S05347
type: SequenceAnnotation
persistent identity: annotation2500062
display id: annotation2500062
version: 1
top level: BBa_K1896017
location: https://synbiohub.org/public/igem/BBa_K1896017/annotation2500062/range2500062/1
component: BBa_S05347

https://synbiohub.org/public/igem/BBa_K1896017/annotation2500062/range2500062/1
type: Range
persistent identity: range2500062
display id: range2500062
version: 1
top level: BBa_K1896017
direction: direction/reverse
start: 3586
end: 3714
orientation: inline

BBa_S05347
type: ComponentDefinition
persistent identity: BBa S05347
display id: BBa_S05347
version: 1
was generated by: iGEM to SBOL conversion
description: B0010:B0012
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T02:32:06Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S05347_sequence/1

BBa_B0012
type: Component
persistent identity: component2495234
display id: component2495234
version: 1
top level: BBa_S05347
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2495232
display id: component2495232
version: 1
top level: BBa_S05347
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2495232
display id: annotation2495232
version: 1
top level: BBa_S05347
location: https://synbiohub.org/public/igem/BBa_S05347/annotation2495232/range2495232/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S05347/annotation2495232/range2495232/1
type: Range
persistent identity: range2495232
display id: range2495232
version: 1
top level: BBa_S05347
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2495234
display id: annotation2495234
version: 1
top level: BBa_S05347
location: https://synbiohub.org/public/igem/BBa_S05347/annotation2495234/range2495234/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S05347/annotation2495234/range2495234/1
type: Range
persistent identity: range2495234
display id: range2495234
version: 1
top level: BBa_S05347
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_S05357
type: ComponentDefinition
persistent identity: BBa S05357
display id: BBa_S05357
version: 1
was generated by: iGEM to SBOL conversion
description: J23105:B0034
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T05:35:04Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J23105
component: BBa_B0034
sequence annotation: BBa_J23105
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S05357_sequence/1

BBa_J23105
type: Component
persistent identity: component2499761
display id: component2499761
version: 1
top level: BBa_S05357
definition: BBa_J23105
access: public

BBa_B0034
type: Component
persistent identity: component2499763
display id: component2499763
version: 1
top level: BBa_S05357
definition: BBa_B0034
access: public

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2499761
display id: annotation2499761
version: 1
top level: BBa_S05357
location: https://synbiohub.org/public/igem/BBa_S05357/annotation2499761/range2499761/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_S05357/annotation2499761/range2499761/1
type: Range
persistent identity: range2499761
display id: range2499761
version: 1
top level: BBa_S05357
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2499763
display id: annotation2499763
version: 1
top level: BBa_S05357
location: https://synbiohub.org/public/igem/BBa_S05357/annotation2499763/range2499763/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05357/annotation2499763/range2499763/1
type: Range
persistent identity: range2499763
display id: range2499763
version: 1
top level: BBa_S05357
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_S05363
type: ComponentDefinition
persistent identity: BBa S05363
display id: BBa_S05363
version: 1
was generated by: iGEM to SBOL conversion
description: K1896008:K1896004
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T05:52:58Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Bob Van Hove
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1896004
component: BBa_K1896008
sequence annotation: BBa_K1896008
sequence annotation: BBa_K1896004
sequence: https://synbiohub.org/public/igem/BBa_S05363_sequence/1

BBa_K1896004
type: Component
persistent identity: component2499865
display id: component2499865
version: 1
top level: BBa_S05363
definition: BBa_K1896004
access: public

BBa_K1896008
type: Component
persistent identity: component2499859
display id: component2499859
version: 1
top level: BBa_S05363
definition: BBa_K1896008
access: public

BBa_K1896008
type: SequenceAnnotation
persistent identity: annotation2499859
display id: annotation2499859
version: 1
top level: BBa_S05363
location: https://synbiohub.org/public/igem/BBa_S05363/annotation2499859/range2499859/1
component: BBa_K1896008

https://synbiohub.org/public/igem/BBa_S05363/annotation2499859/range2499859/1
type: Range
persistent identity: range2499859
display id: range2499859
version: 1
top level: BBa_S05363
direction: direction/reverse
start: 1
end: 3096
orientation: inline

BBa_K1896004
type: SequenceAnnotation
persistent identity: annotation2499865
display id: annotation2499865
version: 1
top level: BBa_S05363
location: https://synbiohub.org/public/igem/BBa_S05363/annotation2499865/range2499865/1
component: BBa_K1896004

https://synbiohub.org/public/igem/BBa_S05363/annotation2499865/range2499865/1
type: Range
persistent identity: range2499865
display id: range2499865
version: 1
top level: BBa_S05363
direction: direction/reverse
start: 3097
end: 3516
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K1896004
type: ComponentDefinition
persistent identity: BBa K1896004
display id: BBa_K1896004
version: 1
was generated by: iGEM to SBOL conversion
description: linker-Streptavidin-His (tetrameric)
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T05:41:27Z
mutable provenance: Part:BBa_K283010
owned by: james
owned by: myers
mutable description: This is an improved version of Part:BBa_K283010, which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: One BsaI site was removed to enable Golden Gate cloning.
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GSTGS linker
sequence annotation: Streptavidin
sequence annotation: BamHI site removed
sequence annotation: His-tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1896004_sequence/1

GSTGS linker
type: SequenceAnnotation
persistent identity: annotation2497040
display id: annotation2497040
version: 1
top level: BBa_K1896004
location: https://synbiohub.org/public/igem/BBa_K1896004/annotation2497040/range2497040/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896004/annotation2497040/range2497040/1
type: Range
persistent identity: range2497040
display id: range2497040
version: 1
top level: BBa_K1896004
direction: direction/forward
start: 1
end: 15
orientation: inline

Streptavidin
type: SequenceAnnotation
persistent identity: annotation2497042
display id: annotation2497042
version: 1
top level: BBa_K1896004
location: https://synbiohub.org/public/igem/BBa_K1896004/annotation2497042/range2497042/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896004/annotation2497042/range2497042/1
type: Range
persistent identity: range2497042
display id: range2497042
version: 1
top level: BBa_K1896004
direction: direction/forward
start: 16
end: 396
orientation: inline

BamHI site removed
type: SequenceAnnotation
persistent identity: annotation2497041
display id: annotation2497041
version: 1
top level: BBa_K1896004
location: https://synbiohub.org/public/igem/BBa_K1896004/annotation2497041/range2497041/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1896004/annotation2497041/range2497041/1
type: Range
persistent identity: range2497041
display id: range2497041
version: 1
top level: BBa_K1896004
direction: direction/forward
start: 60
end: 60
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2497044
display id: annotation2497044
version: 1
top level: BBa_K1896004
location: https://synbiohub.org/public/igem/BBa_K1896004/annotation2497044/range2497044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896004/annotation2497044/range2497044/1
type: Range
persistent identity: range2497044
display id: range2497044
version: 1
top level: BBa_K1896004
direction: direction/forward
start: 397
end: 414
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2497045
display id: annotation2497045
version: 1
top level: BBa_K1896004
location: https://synbiohub.org/public/igem/BBa_K1896004/annotation2497045/range2497045/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1896004/annotation2497045/range2497045/1
type: Range
persistent identity: range2497045
display id: range2497045
version: 1
top level: BBa_K1896004
direction: direction/forward
start: 415
end: 420
orientation: inline

BBa_K1896008
type: ComponentDefinition
persistent identity: BBa K1896008
display id: BBa_K1896008
version: 1
was generated by: iGEM to SBOL conversion
description: INP_RC
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T05:52:47Z
mutable provenance: Part:BBa_K1896001
owned by: james
owned by: myers
mutable description: INPRC is a truncated variant of the ''inaZ'' from ''Pseudomonas syringae'' (Part:BBa_K1896001), the N-terminal domain was removed in order to prevent localisation to the outer membrane, while still maintaining ice nucleation activity. No stop codon is present in this part to allow seamless assembly of fusion proteins.
discontinued: false
dominant: false
experience: experience/None
group u list: _2362_
m user id: 19009
owner id: 19009
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The repeat domain in this part seems to be impossible to create synthetically so PCR was used instead, with primers binding just outside of the repeat region. The absence of a stop codon allows for seamless assembly of fusion proteins.
star: false
creator: Bob Van Hove, Maarten Van Brempt
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: inaZ_RC
sequence annotation: start
sequence annotation: repeat region
sequence: https://synbiohub.org/public/igem/BBa_K1896008_sequence/1

inaZ_RC
type: SequenceAnnotation
persistent identity: annotation2502230
display id: annotation2502230
version: 1
top level: BBa_K1896008
location: https://synbiohub.org/public/igem/BBa_K1896008/annotation2502230/range2502230/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1896008/annotation2502230/range2502230/1
type: Range
persistent identity: range2502230
display id: range2502230
version: 1
top level: BBa_K1896008
direction: direction/forward
start: 1
end: 3096
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2502231
display id: annotation2502231
version: 1
top level: BBa_K1896008
location: https://synbiohub.org/public/igem/BBa_K1896008/annotation2502231/range2502231/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1896008/annotation2502231/range2502231/1
type: Range
persistent identity: range2502231
display id: range2502231
version: 1
top level: BBa_K1896008
direction: direction/forward
start: 1
end: 3
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation2502248
display id: annotation2502248
version: 1
top level: BBa_K1896008
location: https://synbiohub.org/public/igem/BBa_K1896008/annotation2502248/range2502248/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1896008/annotation2502248/range2502248/1
type: Range
persistent identity: range2502248
display id: range2502248
version: 1
top level: BBa_K1896008
direction: direction/forward
start: 22
end: 2949
orientation: inline

https://synbiohub.org/public/igem/BBa_K1896017_sequence/1
type: Sequence
persistent identity: BBa K1896017 sequence
display id: BBa_K1896017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05347_sequence/1
type: Sequence
persistent identity: BBa S05347 sequence
display id: BBa_S05347_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05357_sequence/1
type: Sequence
persistent identity: BBa S05357 sequence
display id: BBa_S05357_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05363_sequence/1
type: Sequence
persistent identity: BBa S05363 sequence
display id: BBa_S05363_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23105_sequence/1
type: Sequence
persistent identity: BBa J23105 sequence
display id: BBa_J23105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1896004_sequence/1
type: Sequence
persistent identity: BBa K1896004 sequence
display id: BBa_K1896004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1896008_sequence/1
type: Sequence
persistent identity: BBa K1896008 sequence
display id: BBa_K1896008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacgg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z