RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
This is an improved version of Part:BBa_K283010, which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.
One BsaI site was removed to enable Golden Gate cloning.
INPRC is a truncated variant of the ''inaZ'' from ''Pseudomonas syringae'' (Part:BBa_K1896001), the N-terminal domain was removed in order to prevent localisation to the outer membrane, while still maintaining ice nucleation activity. No stop codon is present in this part to allow seamless assembly of fusion proteins.
The repeat domain in this part seems to be impossible to create synthetically so PCR was used instead, with primers binding just outside of the repeat region. The absence of a stop codon allows for seamless assembly of fusion proteins.
Conversion of the iGEM parts registry to SBOL2.1