BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_S05347
1
BBa_S05347
B0010:B0012
2016-10-11T11:00:00Z
2016-10-12T02:32:06Z
false
false
_9_
19009
19009
9
false
false
Bob Van Hove
component2495234
1
BBa_B0012
component2495232
1
BBa_B0010
annotation2495232
1
BBa_B0010
range2495232
1
1
80
annotation2495234
1
BBa_B0012
range2495234
1
89
129
BBa_K1896019
1
BBa_K1896019
pXS-GG
2016-10-12T11:00:00Z
2016-10-14T05:28:19Z
todo
todo
false
false
_2362_
19009
19009
9
false
todo
false
Bob Van Hove, Maarten Van Brempt
component2500085
1
BBa_S05355
component2500086
1
BBa_K1896009
component2500093
1
BBa_S05347
annotation2500085
1
BBa_S05355
range2500085
1
1
55
annotation2500086
1
BBa_K1896009
range2500086
1
62
83
annotation2500093
1
BBa_S05347
range2500093
1
92
220
BBa_S05355
1
BBa_S05355
J23119:B0034
2016-10-12T11:00:00Z
2016-10-13T12:33:03Z
false
false
_9_
19009
19009
9
false
false
Bob Van Hove
component2499223
1
BBa_B0034
component2499221
1
BBa_J23119
annotation2499221
1
BBa_J23119
range2499221
1
1
35
annotation2499223
1
BBa_B0034
range2499223
1
44
55
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1896009
1
BBa_K1896009
BsaI MCS
2016-10-12T11:00:00Z
2016-10-13T12:30:38Z
Fully synthetic.
BsaI cloning site for Golden Gate cloning.
false
false
_2362_
19009
19009
9
false
The spacer inbetween the diverging BsaI sites was designed for minimal secondary structure to allow this part to be built from annealed oligonucleotides.
false
Bob Van Hove
annotation2502106
1
BsaI
range2502106
1
5
10
annotation2502107
1
BsaI
range2502107
1
16
21
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_S05355_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_S05347_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1896009_sequence
1
atgcgagaccaaaaaggtctcc
BBa_K1896019_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcgagaccaaaaaggtctcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z