LOCUS       BBa_K1897001             760 bp    DNA     linear       03-May-2024
DEFINITION  HasA hemophore expression unit
ACCESSION   BBa_K1897001
VERSION     BBa_K1897001.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..41
                     /label=Start codon
     misc_feature    621..623
                     /label=Stop codon
     misc_feature    603..620
                     /label=Hexa histadine tag
     CDS             39..602
                     /label=HasA
     CDS             39..623
                     /label=BBa_K1897000
     stem_loop       643..686
                     /label=stem_loop
     misc_feature    632..711
                     /label=BBa_B0010
     terminator      632..711
                     /label=BBa_B0010
     stem_loop       727..746
                     /label=T7 TE
     misc_feature    753..753
                     /label=stop
     polyA_site      747..760
                     /label=polya
     misc_feature    720..760
                     /label=BBa_B0012
     terminator      720..760
                     /label=BBa_B0012
     terminator      632..760
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggcattttca gtcaattatg
       61 acagcagctt cggcggttac agcattcatg actatctggg ccagtgggct tcgacattcg
      121 gtgatgtcaa tcacaccaac ggcaatgtca ccgacgccaa cagcggcggc ttctatggcg
      181 gcagcctgtc cggtagtcag tatgccatca gtagcacggc aaaccaggtg accgccttcg
      241 tggcgggcgg caacctgacc tacacgctgt tcaacgaacc ggcgcatacc ttgtacggcc
      301 aactggattc cctgtccttc ggcgacggtt tgagcggtgg cgatacatcg ccgtacagca
      361 tccaggtgcc tgacgtcagc ttcggcggcc tgaacctcag cagcctacaa gcgcagggtc
      421 atgatggcgt ggtgcaccag gtggtgtacg gcctgatgtc cggcgatacc ggcgcgctgg
      481 agaccgcgct gaacggcatc ctcgacgact acggcctgag cgtcaactcc accttcgatc
      541 aggtggcggc ggcgacggcg gtgggcgtgc agcacgccga cagcccggaa ctgctggcgg
      601 cccaccatca ccaccatcat tgatactaga gccaggcatc aaataaaacg aaaggctcag
      661 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      721 cacactggct caccttcggg tgggcctttc tgcgtttata
//