BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1897001
1
BBa_K1897001
HasA hemophore expression unit
2016-10-08T11:00:00Z
2016-10-10T03:37:25Z
The HasA coding sequence is originally found in the Serratia marcescens and to construct this part, we used the HasA gene that is from the part BBa K1897000 which was synthesised based on the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2). The Promoter, RBS and terminator are biobrick parts that were obtained from the Distribution kits from iGEM.
The HasA hemophore (19 kDa monomer) is originally from the Serratia marcescens and used in their heme uptake pathway. When bound to heme, the HasA hemophore is able to trigger expression of genes through the Has system. The heme-bound HasA hemophore is able to bind to a membrane receptor HasR, a component of the signalling cascade that regulates expression of the genes in the has operon via HasS and HasI. When HasR is activated, it causes a conformation change in HasS, an anti-sigma factor. This causes the release of the sigma factor, HasI, initially bound to it. HasI then goes on to bind to a specific promoter to trigger expression of genes under the control of that promoter. This is a composite part created from the coding sequence of HasA (BBa K1897000) with the addition of Bba_K525998, which contains a promoter and Ribosome Binding Site (RBS), and Bba_B0015, which contains a double terminator. The addition of these parts make BBa K1897001 a functional unit that can be used for expression, unlike BBa K1897000 which lacks the promoter, RBS and terminator and hence cannot be used on its own for expression.
false
false
_2363_
29428
29428
9
false
The presence of a hexa histidine tag would allow for easy protein purification and detection using anti-his antibodies. The promoter BBa_K525998 was chosen as we wished to induce high levels of HasA for purification thus we required a T7 promoter that could be used to induce expression at high levels in E. coli strain BL21(DE3). The terminator BBa_B0015 was chosen as it is commonly used and has a high efficacy.
false
Choi Yan Ru
component2489750
1
BBa_K525998
component2489762
1
BBa_B0015
component2489755
1
BBa_K1897000
annotation2489750
1
BBa_K525998
range2489750
1
1
32
annotation2489762
1
BBa_B0015
range2489762
1
632
760
annotation2489755
1
BBa_K1897000
range2489755
1
39
623
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2127788
1
B0034
range2127788
1
21
32
annotation2129433
1
RBS
range2129433
1
24
27
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1897000
1
BBa_K1897000
HasA hemophore coding sequence
2016-10-06T11:00:00Z
2016-10-09T10:00:00Z
c
c
false
false
_2363_
29428
29428
9
false
d
false
Choi Yan Ru
annotation2488976
1
Start codon
range2488976
1
1
3
annotation2488995
1
HasA
range2488995
1
1
564
annotation2488977
1
Stop codon
range2488977
1
583
585
annotation2488978
1
Hexa histadine tag
range2488978
1
565
582
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1897001_sequence
1
taatacgactcactatagggaaagaggagaaatactagatggcattttcagtcaattatgacagcagcttcggcggttacagcattcatgactatctgggccagtgggcttcgacattcggtgatgtcaatcacaccaacggcaatgtcaccgacgccaacagcggcggcttctatggcggcagcctgtccggtagtcagtatgccatcagtagcacggcaaaccaggtgaccgccttcgtggcgggcggcaacctgacctacacgctgttcaacgaaccggcgcataccttgtacggccaactggattccctgtccttcggcgacggtttgagcggtggcgatacatcgccgtacagcatccaggtgcctgacgtcagcttcggcggcctgaacctcagcagcctacaagcgcagggtcatgatggcgtggtgcaccaggtggtgtacggcctgatgtccggcgataccggcgcgctggagaccgcgctgaacggcatcctcgacgactacggcctgagcgtcaactccaccttcgatcaggtggcggcggcgacggcggtgggcgtgcagcacgccgacagcccggaactgctggcggcccaccatcaccaccatcattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1897000_sequence
1
atggcattttcagtcaattatgacagcagcttcggcggttacagcattcatgactatctgggccagtgggcttcgacattcggtgatgtcaatcacaccaacggcaatgtcaccgacgccaacagcggcggcttctatggcggcagcctgtccggtagtcagtatgccatcagtagcacggcaaaccaggtgaccgccttcgtggcgggcggcaacctgacctacacgctgttcaacgaaccggcgcataccttgtacggccaactggattccctgtccttcggcgacggtttgagcggtggcgatacatcgccgtacagcatccaggtgcctgacgtcagcttcggcggcctgaacctcagcagcctacaagcgcagggtcatgatggcgtggtgcaccaggtggtgtacggcctgatgtccggcgataccggcgcgctggagaccgcgctgaacggcatcctcgacgactacggcctgagcgtcaactccaccttcgatcaggtggcggcggcgacggcggtgggcgtgcagcacgccgacagcccggaactgctggcggcccaccatcaccaccatcattga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z