LOCUS BBa_K1897007 7089 bp DNA linear 26-Nov-2024 DEFINITION Complete Has operon (controlling expression of luxR) ACCESSION BBa_K1897007 VERSION BBa_K1897007.1 FEATURES Location/Qualifiers misc_feature 7..39 /label=BBa_K1897006 misc_feature 45..56 /label=BBa_B0034 CDS 57..837 /label=LuxR misc_feature 57..837 /label=BBa_C0062 misc_feature 219..219 /label=T misc_feature 537..537 /label=A misc_feature 838..849 /label=BBa_B0034 misc_feature 850..1555 /label=BBa_E1010 (mRFP) CDS 850..1555 /label=mRFP misc_feature 1556..1635 /label=BBa_B0010 misc_feature 1556..1684 /label=BBa_B0015 misc_feature 1644..1684 /label=BBa_B0012 misc_feature 1685..1719 /label=BBa_J23119 misc_feature 1732..1743 /label=BBa_B0034 misc_feature 1744..1746 /label=Start codon CDS 1744..4440 /label=HasR misc_feature 1744..4440 /label=BBa_K1897002 (without stop codon) misc_feature 4441..4458 /label=Hexa histidine tag misc_feature 4459..4461 /label=Stop codon misc_feature 4482..4493 /label=BBa_B0034 misc_feature 4494..4496 /label=Start codon misc_feature 4494..5444 /label=BBa_K1897003 (without stop codon) CDS 4494..5444 /label=HasS misc_feature 5445..5462 /label=Hexa Histidine tag misc_feature 5463..5465 /label=Stop Codon misc_feature 5478..5489 /label=BBa_B0034 misc_feature 5490..5987 /label=BBa_K1897005 (without stop codon) CDS 5490..5987 /label=HasI misc_feature 5490..5492 /label=Start codon misc_feature 5988..6005 /label=Hexa Histidine tag misc_feature 6006..6008 /label=Stop codon misc_feature 6015..6957 /label=BBa_P1002 promoter 6047..6052 /label=ampR -35 promoter 6075..6080 /label=ampR -10 RBS 6090..6094 /label=ampR RBS CDS 6094..6957 /label=ampicillin resistance misc_feature 6961..7089 /label=BBa_B0015 misc_feature 6961..7040 /label=BBa_B0010 misc_feature 7049..7089 /label=BBa_B0012 ORIGIN 1 ctcgagtttt acgggtttat ccgcgctcag acgtcttaac ctataaagag gagaaaatga 61 aaaacataaa tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca 121 ataatgatat taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt 181 tactcgcgat catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt 241 accctaaaaa atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag 301 tagattattc taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg 361 taaataaaaa atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt 421 ttagtttccc tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag 481 aaaaagacaa ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg 541 ttccttctct agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg 601 atttaaccaa aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg 661 atatttcaaa aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc 721 aaatgaaact caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag 781 caattgattg cccatacttt aaaaattaat aacactgata gtgctagtgt agatcacaaa 841 gaggagaaaa tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt 901 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg 961 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct 1021 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct 1081 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg 1141 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag 1201 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag 1261 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg 1321 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt 1381 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc 1441 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct 1501 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgcccagg 1561 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1621 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1681 tatattgaca gctagctcag tcctaggtat aatgctagca gatctgggcc caaagaggag 1741 aaaatgttta ttcacaaggg aaccacgccg gccggccgat tggccacggc ggtacgcgcc 1801 gcgctggcgg cgatgatgtt gactcagccg gcggtagcgc tcgccgccca ggctgaggcg 1861 agcagcgcgc aggccgcgca gcaaaagaat ttcaacattg cggcgcagcc gctacaaagc 1921 gccatgttgc gcttcgccga gcaggccggc atgcaggtgt tttttgacga ggtgaaactc 1981 gatggcatgc aggcggcggc gctgaacggc agcatgagcg tagaacaggg actgcggcgc 2041 ttgatcggcg gcaatccggt ggctttccgc ctacaaccgc aggggcagat cgtattgagc 2101 cggctgccga cggcgaacgg cgacggtggc gcgctggcgt tggacagcct gacggtgctg 2161 ggcgccggcg gcaacaacgc caacgattgg gtttacgacg aaccgcgctc ggtcagcgtc 2221 atcagccgcg aacaaatgga caaccgcccg gcgcgacacg cggccgatat tctggagcag 2281 actacgggag cctattccag cgtcagccag caagatcctg cgctgtcggt caacatccgc 2341 ggcatacaag actatggccg ggtgaacatg aatatcgacg gcatgcggca gaattttcaa 2401 aagagcggcc atggccaacg taatggcacc atgtacatcg attccgaact gctgtccggc 2461 gtgaccatcg acaagggcac caccggcggc atgggcagcg ccggcacgct cggcggcatc 2521 gccaccttca ataccgtcag cgcgagcgat ttcctggcgc cgggcaaaga gctgggcggc 2581 aagctgcacg ccagcaccgg cgataacggc actcacttca tcggcagcgg catactggca 2641 ttgggcaacg aaaccggcga tatcctgctg gccgccagcg aacgccacct cggcgactat 2701 tggcccggca acaagggcga catcggcaac attcgcatca ataacgacac cggcaattac 2761 gatcgctacg ccgagagcat caagaacaac aaaatccccg acacccatta ccgcatgcac 2821 tcgcggctgg ccaaggtggg ctggaatctg cccgccaacc agcgcctaca actgagttat 2881 ctacaaaccc agaccgcatc gccgatcgcc ggcaccttga ctaacctggg cactcgcccg 2941 ccctatgaac tgggctggaa acgcaccggc tacaccgatg tgatggcgcg caacgcggcg 3001 ttcgactaca gcctggcgcc ggaagacgtc gactggctcg attttcaggc caagctgtat 3061 tacgtcgata ctcaggatga cagcgacacc tacagcacca gttcgctgct ggacaacggc 3121 tacgcgacgc gcacccgcct gcgtacctat ggcgcacagg cgcaaaacac ctcgcgcttc 3181 agcctggcgc cggggcatga cttccgcgcc aattacgggc tggagttcta ttacgacaaa 3241 gcgaccagcg actcttcccg ccaaggcatg gaaggggtga cgccggccgg caaccgttcg 3301 gtagccagcc tgttcgccaa tctgacctac gactacgacg gctggctgac gttggaaggc 3361 gggctgcgtt acgaccgcta tcgcctgcgc ggccagaccg gcctgagcta tccggatttg 3421 gccaaggatg ggcaacgcta cacgattgac aatccatgca aagcgttgcg tctgaccggc 3481 tgttcaacca ccacccgcga agattgggac gtggatcgcg atcagggcaa gctgtcgccg 3541 acgctggcgg tggcggtgcg ccccggcgtg gagtggctgg agctgtatac cacctacggc 3601 aaatcctggc ggccgccggc gatcactgaa acgctgacca acggcagcgc gcacagttct 3661 tccacgcaat accccaatcc gttcttgcag cccgagcgtt cgcgcgcctg ggaagtcggg 3721 ttcaacgtgc agcagccgga tctgtggttt gagggcgatc ggttggtggc caaggtgggc 3781 tacttcgaca ccaaagtgga taactacatc aacctggcga tagaccgcaa taaaccgggg 3841 ctggtgcagc cgagcatcgg caatgccgct tacgtcaaca atctgtcgaa aacccgcttc 3901 cgcgggctgg agtaccagct caactatgac gccggggtgt tctacgccga cctgacctac 3961 acccacatga tcggcaaaaa cgagttctgc tcgaacaagg cctggttggg agggcgtctg 4021 cgctacggcg acggctcgcg ccgcgggaac ttctatgttg agcctgatgc cgcgtccaat 4081 gacttcgtca cgtgtgatgg ggggacgcag ttcggctccg ccgcctacct gccgggcgat 4141 cgcggttcgg tgacgctggg cgggcgtgcc ttcgatcgca agctggacgc cggggtgacc 4201 gtacgctttg cgccgggtta tcaggacagc tcggtaccgt ccaactaccc gtacctggcc 4261 gactggccga agtacaccct gttcgatctg tacgccagct acaagctgac cgacagcctg 4321 acgctgcgcg gctcggtgga gaacctgacc aaccgcgctt atgtcgtcag ctacggcgag 4381 acgttggcca ataccctggg gcgcggccgc accgtgcagg gcggggtgga ataccgtttt 4441 caccatcacc accatcatta agggcccggc gcgccggatc caaagaggag aaaatgaaac 4501 cgctaaccgc acaggcgcga cagcaggcgg ccgcctgggc cgtgagtctg gccgaagggg 4561 cgctgccggc cgatcggcgg cgagcgttgg acgcctggct ggccgccgat ccgcagcatc 4621 cggcggcgct gcgtcaggct cgcctgctat ggacgacgct tgggcaactg ccgcctgagc 4681 agcggcaggc gttacaaccg caggtggcgg cgctgaaaac gccatctcgc catcgatggc 4741 cccgctgggc ggtggcagcg gcggtggcgc tcgccgtttc attcggcgtt tatcgcgggc 4801 cggatctgtg gatccgccta caagccgatt atcaaaccgt tcgcggtgag gtgcgcgaag 4861 ttgcgttgcc ggacggcagc cgggtggcgc tggacagcgg cagcgcgatc gcgctggcgt 4921 attccgcaga cgagcgcaag gtgcggctgc tgagcggcgc cgcctatttt atcgtcgcgc 4981 cgttgggcgg ggcggaaagc cgccctttca gggtggaggc gaaaaacggc gtcacccagg 5041 cgctgggcac cgagttcagc gtggcgcgca ctgaacaggg cgtcgacgtc agcgtgcatc 5101 agcacagcgt gcgggtgacg cttgagcgcg gcgagcgctc gctggtggtc gcgcaacgcc 5161 aggcggcgca ttaccgcaac gggcagctac aactgacgcg gcaaacggcc ggtgatgacg 5221 cctggcgacg cggctggctg gtgatcgacc gccaaccgct gacgcaggcg ctcgcgcagc 5281 tcaaccgcta tcgcgggacc cgcattgtgg cggttaatcc cgcgctaaga acgcgcaccg 5341 tgagcggtgt gttcgcgctc aataaactcg acgacggcgt cggcgccatt cgtcaggaac 5401 tctccgcccg ccaactgaat ctccccggca tcacattgct ttaccaccat caccaccatc 5461 attaaggatc ccatatgaaa gaggagaaaa tgtccaattc cgagttgcag gcgctgtttc 5521 tgcgccatat gcggccgctg caagcctatt tgaacgccaa actgcgcgac cctcagctgg 5581 ccgcggattt ggcgcaggaa agttttaccc gccttaccga gcagtatccg cagggtaaca 5641 ttctcgatat tgaggcctac ctgtataaaa ccgccaagaa tctgatgctc gaccatctgc 5701 gccagcagca gcggcggcag accgaagcgg tcgaagacga cattctggcg cagtatcctt 5761 cgggtgaacc ggcgcttgaa caacgggcga tcgatagcca actgctacaa ttgttacagc 5821 aggcgctggc ggacatgccg ccgcgcacgc agcagatttt ccgccttaac cggctcgatg 5881 gcctgaccca ggcgcaggtg gcggcgcagc tgggcgtgtc gctcagcacg gtggaaaaac 5941 acctggccag cgcgctggag cggctgatgg cgcggatgga agaacaacac catcaccacc 6001 atcattaaca tatgctaaat acattcaaat atctatccgc tcatgagaca ataaccctga 6061 taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc 6121 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 6181 aaagtaaaag atgccgaaga tcagttgggt gcacgtgtgg gttacatcga actggacctc 6241 aacagcggta agattcttga gagttttcgc cccgaagaac gtttcccaat gatgagcact 6301 tttaaagttc tgctctgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 6361 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 6421 catcttacgg acggcatgac agtacgcgaa ttatgcagcg ctgccataac catgagtgat 6481 aacacggcgg ccaacttact tctgacaacg atcggaggac cgaaggagct taccgctttt 6541 ttgcacaaca tgggtgatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 6601 gccataccaa acgacgagcg tgacaccacg atgcctgtag ctatggcaac aacgttgcgc 6661 aaactcttaa ctggcgaact tcttactctc gcttcccggc aacaattaat agactggatg 6721 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 6781 gctgataaat ctggagccgg tgagcgtggg tcccgcggta ttattgcagc cctggggcca 6841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagccaggc aactatggac 6901 gaacgtaatc gccagatcgc tgagataggt gcctccctga ttaagcattg gtaataataa 6961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 7021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 7081 gcgtttata //