##gff-version 3
##sequence-region BBa_K1897007 7 7089
BBa_K1897007	.	CDS	6094	6957	.	+	0	ID=annotation2491071;Name=ampicillin resistance
BBa_K1897007	.	engineered_region	6961	7089	.	+	0	ID=annotation2491072;Name=BBa_B0015
BBa_K1897007	.	engineered_region	838	849	.	+	0	ID=annotation2491042;Name=BBa_B0034
BBa_K1897007	.	promoter	6075	6080	.	+	0	ID=annotation2491069;Name=ampR -10
BBa_K1897007	.	engineered_region	1556	1635	.	+	0	ID=annotation2491045;Name=BBa_B0010
BBa_K1897007	.	point_mutation	537	537	.	+	0	ID=annotation2491041;Name=A
BBa_K1897007	.	stop_codon	6006	6008	.	+	0	ID=annotation2491066;Name=Stop codon
BBa_K1897007	.	start_codon	4494	4496	.	+	0	ID=annotation2491056;Name=Start codon
BBa_K1897007	.	engineered_region	1685	1719	.	+	0	ID=annotation2491048;Name=BBa_J23119
BBa_K1897007	.	tag	5445	5462	.	+	0	ID=annotation2491059;Name=Hexa Histidine tag
BBa_K1897007	.	CDS	850	1555	.	+	0	ID=annotation2491044;Name=mRFP
BBa_K1897007	.	stop_codon	4459	4461	.	+	0	ID=annotation2491054;Name=Stop codon 
BBa_K1897007	.	tag	4441	4458	.	+	0	ID=annotation2491053;Name=Hexa histidine tag
BBa_K1897007	.	point_mutation	219	219	.	+	0	ID=annotation2491040;Name=T
BBa_K1897007	.	promoter	6047	6052	.	+	0	ID=annotation2491068;Name=ampR -35
BBa_K1897007	.	engineered_region	6961	7040	.	+	0	ID=annotation2491073;Name=BBa_B0010
BBa_K1897007	.	engineered_region	1744	4440	.	+	0	ID=annotation2491052;Name=BBa_K1897002 (without stop codon)
BBa_K1897007	.	tag	5988	6005	.	+	0	ID=annotation2491065;Name=Hexa Histidine tag
BBa_K1897007	.	engineered_region	57	837	.	+	0	ID=annotation2491039;Name=BBa_C0062
BBa_K1897007	.	engineered_region	7049	7089	.	+	0	ID=annotation2491074;Name=BBa_B0012
BBa_K1897007	.	CDS	4494	5444	.	+	0	ID=annotation2491058;Name=HasS
BBa_K1897007	.	engineered_region	6015	6957	.	+	0	ID=annotation2491067;Name=BBa_P1002
BBa_K1897007	.	engineered_region	4482	4493	.	+	0	ID=annotation2491055;Name=BBa_B0034
BBa_K1897007	.	engineered_region	1732	1743	.	+	0	ID=annotation2491049;Name=BBa_B0034
BBa_K1897007	.	ribosome_entry_site	6090	6094	.	+	0	ID=annotation2491070;Name=ampR RBS
BBa_K1897007	.	start_codon	1744	1746	.	+	0	ID=annotation2491050;Name=Start codon
BBa_K1897007	.	CDS	1744	4440	.	+	0	ID=annotation2491051;Name=HasR
BBa_K1897007	.	engineered_region	4494	5444	.	+	0	ID=annotation2491057;Name=BBa_K1897003 (without stop codon)
BBa_K1897007	.	engineered_region	5490	5987	.	+	0	ID=annotation2491062;Name=BBa_K1897005 (without stop codon)
BBa_K1897007	.	stop_codon	5463	5465	.	+	0	ID=annotation2491060;Name=Stop Codon
BBa_K1897007	.	CDS	57	837	.	+	0	ID=annotation2491038;Name=LuxR
BBa_K1897007	.	engineered_region	1556	1684	.	+	0	ID=annotation2491046;Name=BBa_B0015
BBa_K1897007	.	engineered_region	1644	1684	.	+	0	ID=annotation2491047;Name=BBa_B0012
BBa_K1897007	.	start_codon	5490	5492	.	+	0	ID=annotation2491064;Name=Start codon
BBa_K1897007	.	engineered_region	45	56	.	+	0	ID=annotation2491037;Name=BBa_B0034
BBa_K1897007	.	engineered_region	5478	5489	.	+	0	ID=annotation2491061;Name=BBa_B0034
BBa_K1897007	.	engineered_region	850	1555	.	+	0	ID=annotation2491043;Name=BBa_E1010 (mRFP)
BBa_K1897007	.	engineered_region	7	39	.	+	0	ID=annotation2491036;Name=BBa_K1897006
BBa_K1897007	.	CDS	5490	5987	.	+	0	ID=annotation2491063;Name=HasI
>BBa_K1897007
ctcgagttttacgggtttatccgcgctcagacgtcttaacctataaagaggagaaaatgaaaaacataaatgccgacga
cacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaa
atggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagata
attaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactc
caatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcg
aaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcac
attcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagt
tgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcg
tgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaa
ccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattg
cccatactttaaaaattaataacactgatagtgctagtgtagatcacaaagaggagaaaatggcttcctccgaagacgt
tatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggt
gaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggaca
tcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtc
cttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctcc
ctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaa
aaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtct
gaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggt
gcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctg
aaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatattgacagctagctcagtcctaggtataatgctagcagatctgggcccaaagagg
agaaaatgtttattcacaagggaaccacgccggccggccgattggccacggcggtacgcgccgcgctggcggcgatgat
gttgactcagccggcggtagcgctcgccgcccaggctgaggcgagcagcgcgcaggccgcgcagcaaaagaatttcaac
attgcggcgcagccgctacaaagcgccatgttgcgcttcgccgagcaggccggcatgcaggtgttttttgacgaggtga
aactcgatggcatgcaggcggcggcgctgaacggcagcatgagcgtagaacagggactgcggcgcttgatcggcggcaa
tccggtggctttccgcctacaaccgcaggggcagatcgtattgagccggctgccgacggcgaacggcgacggtggcgcg
ctggcgttggacagcctgacggtgctgggcgccggcggcaacaacgccaacgattgggtttacgacgaaccgcgctcgg
tcagcgtcatcagccgcgaacaaatggacaaccgcccggcgcgacacgcggccgatattctggagcagactacgggagc
ctattccagcgtcagccagcaagatcctgcgctgtcggtcaacatccgcggcatacaagactatggccgggtgaacatg
aatatcgacggcatgcggcagaattttcaaaagagcggccatggccaacgtaatggcaccatgtacatcgattccgaac
tgctgtccggcgtgaccatcgacaagggcaccaccggcggcatgggcagcgccggcacgctcggcggcatcgccacctt
caataccgtcagcgcgagcgatttcctggcgccgggcaaagagctgggcggcaagctgcacgccagcaccggcgataac
ggcactcacttcatcggcagcggcatactggcattgggcaacgaaaccggcgatatcctgctggccgccagcgaacgcc
acctcggcgactattggcccggcaacaagggcgacatcggcaacattcgcatcaataacgacaccggcaattacgatcg
ctacgccgagagcatcaagaacaacaaaatccccgacacccattaccgcatgcactcgcggctggccaaggtgggctgg
aatctgcccgccaaccagcgcctacaactgagttatctacaaacccagaccgcatcgccgatcgccggcaccttgacta
acctgggcactcgcccgccctatgaactgggctggaaacgcaccggctacaccgatgtgatggcgcgcaacgcggcgtt
cgactacagcctggcgccggaagacgtcgactggctcgattttcaggccaagctgtattacgtcgatactcaggatgac
agcgacacctacagcaccagttcgctgctggacaacggctacgcgacgcgcacccgcctgcgtacctatggcgcacagg
cgcaaaacacctcgcgcttcagcctggcgccggggcatgacttccgcgccaattacgggctggagttctattacgacaa
agcgaccagcgactcttcccgccaaggcatggaaggggtgacgccggccggcaaccgttcggtagccagcctgttcgcc
aatctgacctacgactacgacggctggctgacgttggaaggcgggctgcgttacgaccgctatcgcctgcgcggccaga
ccggcctgagctatccggatttggccaaggatgggcaacgctacacgattgacaatccatgcaaagcgttgcgtctgac
cggctgttcaaccaccacccgcgaagattgggacgtggatcgcgatcagggcaagctgtcgccgacgctggcggtggcg
gtgcgccccggcgtggagtggctggagctgtataccacctacggcaaatcctggcggccgccggcgatcactgaaacgc
tgaccaacggcagcgcgcacagttcttccacgcaataccccaatccgttcttgcagcccgagcgttcgcgcgcctggga
agtcgggttcaacgtgcagcagccggatctgtggtttgagggcgatcggttggtggccaaggtgggctacttcgacacc
aaagtggataactacatcaacctggcgatagaccgcaataaaccggggctggtgcagccgagcatcggcaatgccgctt
acgtcaacaatctgtcgaaaacccgcttccgcgggctggagtaccagctcaactatgacgccggggtgttctacgccga
cctgacctacacccacatgatcggcaaaaacgagttctgctcgaacaaggcctggttgggagggcgtctgcgctacggc
gacggctcgcgccgcgggaacttctatgttgagcctgatgccgcgtccaatgacttcgtcacgtgtgatggggggacgc
agttcggctccgccgcctacctgccgggcgatcgcggttcggtgacgctgggcgggcgtgccttcgatcgcaagctgga
cgccggggtgaccgtacgctttgcgccgggttatcaggacagctcggtaccgtccaactacccgtacctggccgactgg
ccgaagtacaccctgttcgatctgtacgccagctacaagctgaccgacagcctgacgctgcgcggctcggtggagaacc
tgaccaaccgcgcttatgtcgtcagctacggcgagacgttggccaataccctggggcgcggccgcaccgtgcagggcgg
ggtggaataccgttttcaccatcaccaccatcattaagggcccggcgcgccggatccaaagaggagaaaatgaaaccgc
taaccgcacaggcgcgacagcaggcggccgcctgggccgtgagtctggccgaaggggcgctgccggccgatcggcggcg
agcgttggacgcctggctggccgccgatccgcagcatccggcggcgctgcgtcaggctcgcctgctatggacgacgctt
gggcaactgccgcctgagcagcggcaggcgttacaaccgcaggtggcggcgctgaaaacgccatctcgccatcgatggc
cccgctgggcggtggcagcggcggtggcgctcgccgtttcattcggcgtttatcgcgggccggatctgtggatccgcct
acaagccgattatcaaaccgttcgcggtgaggtgcgcgaagttgcgttgccggacggcagccgggtggcgctggacagc
ggcagcgcgatcgcgctggcgtattccgcagacgagcgcaaggtgcggctgctgagcggcgccgcctattttatcgtcg
cgccgttgggcggggcggaaagccgccctttcagggtggaggcgaaaaacggcgtcacccaggcgctgggcaccgagtt
cagcgtggcgcgcactgaacagggcgtcgacgtcagcgtgcatcagcacagcgtgcgggtgacgcttgagcgcggcgag
cgctcgctggtggtcgcgcaacgccaggcggcgcattaccgcaacgggcagctacaactgacgcggcaaacggccggtg
atgacgcctggcgacgcggctggctggtgatcgaccgccaaccgctgacgcaggcgctcgcgcagctcaaccgctatcg
cgggacccgcattgtggcggttaatcccgcgctaagaacgcgcaccgtgagcggtgtgttcgcgctcaataaactcgac
gacggcgtcggcgccattcgtcaggaactctccgcccgccaactgaatctccccggcatcacattgctttaccaccatc
accaccatcattaaggatcccatatgaaagaggagaaaatgtccaattccgagttgcaggcgctgtttctgcgccatat
gcggccgctgcaagcctatttgaacgccaaactgcgcgaccctcagctggccgcggatttggcgcaggaaagttttacc
cgccttaccgagcagtatccgcagggtaacattctcgatattgaggcctacctgtataaaaccgccaagaatctgatgc
tcgaccatctgcgccagcagcagcggcggcagaccgaagcggtcgaagacgacattctggcgcagtatccttcgggtga
accggcgcttgaacaacgggcgatcgatagccaactgctacaattgttacagcaggcgctggcggacatgccgccgcgc
acgcagcagattttccgccttaaccggctcgatggcctgacccaggcgcaggtggcggcgcagctgggcgtgtcgctca
gcacggtggaaaaacacctggccagcgcgctggagcggctgatggcgcggatggaagaacaacaccatcaccaccatca
ttaacatatgctaaatacattcaaatatctatccgctcatgagacaataaccctgataaatgcttcaataatattgaaa
aaggaagaatatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgct
cacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctca
acagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtgg
cgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgag
tactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtg
ataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtga
tcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcct
gtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagact
ggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctgg
agccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctac
acgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggt
aataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata