BBa_K1897009
1
BBa_K1897009
LuxI
2016-10-09T11:00:00Z
2016-10-10T11:32:29Z
LuxI was obtained from biobrick part <partinfo>BBa_C0061</partinfo>, which was derived from <i>Vibrio fischeri</i>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, LuxI coding sequence <partinfo>BBa_C0061</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
false
false
_2363_
29432
29432
9
false
There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag.
false
Ang Shi Hui
annotation2491097
1
HA tag
range2491097
1
707
733
annotation2491093
1
BBa_R0062
range2491093
1
13
67
annotation2491096
1
BBa_B0015
range2491096
1
737
865
annotation2491094
1
BBa_B0030
range2491094
1
68
82
annotation2491095
1
BBa_C0061
range2491095
1
95
706
annotation2491098
1
Stop Codon
range2491098
1
734
736
BBa_K1897009_sequence
1
cggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z