BBa_K1897008 1 BBa_K1897008 LuxR 2016-10-09T11:00:00Z 2016-10-10T11:16:42Z This part was made based on the genome of Vibrio fischeri. This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015. This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI. This also produces light due to the production of LuxCDABE. Transcription of operonL leads to the production of LuxR. In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon. This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). It also consists of an enzyme cut site XbaI after the biobricks prefix and a KpnI site before the biobricks suffix. false false _2363_ 29432 29432 9 false There is also one HA tag available for characterisation of the protein produced via western blot. false Ang Shi Hui annotation2491080 1 rrnBT1 range2491080 1 843 914 annotation2491077 1 BBa_C0062 range2491077 1 63 812 annotation2491075 1 BBa_J23119 range2491075 1 1 35 annotation2491076 1 BBa_B0030 range2491076 1 36 50 annotation2491079 1 Stop Codon range2491079 1 840 842 annotation2491081 1 BBa_B0012 range2491081 1 923 963 annotation2491078 1 HA tag range2491078 1 813 839 BBa_K1897015 1 BBa_K1897015 LuxR + LuxI 2016-10-09T11:00:00Z 2016-10-10T01:31:43Z These parts are derived from <i>Vibrio fischeri</i>. This part is made by 3A ligation of LuxR <partinfo>BBa_K1897008</partinfo> and LuxI <partinfo>BBa_K1897009</partinfo>. false false _2363_ 29432 29432 9 false There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot. false Ang Shi Hui component2491200 1 BBa_K1897008 component2491207 1 BBa_K1897009 annotation2491207 1 BBa_K1897009 range2491207 1 984 1860 annotation2491200 1 BBa_K1897008 range2491200 1 1 975 BBa_K1897009 1 BBa_K1897009 LuxI 2016-10-09T11:00:00Z 2016-10-10T11:32:29Z LuxI was obtained from biobrick part <partinfo>BBa_C0061</partinfo>, which was derived from <i>Vibrio fischeri</i>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>. This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, LuxI coding sequence <partinfo>BBa_C0061</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>. false false _2363_ 29432 29432 9 false There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag. false Ang Shi Hui annotation2491094 1 BBa_B0030 range2491094 1 68 82 annotation2491097 1 HA tag range2491097 1 707 733 annotation2491096 1 BBa_B0015 range2491096 1 737 865 annotation2491095 1 BBa_C0061 range2491095 1 95 706 annotation2491098 1 Stop Codon range2491098 1 734 736 annotation2491093 1 BBa_R0062 range2491093 1 13 67 BBa_K1897009_sequence 1 cggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcg BBa_K1897015_sequence 1 ttgacagctagctcagtcctaggtataatgctagcattaaagaggagaaaccggtcgaccggatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattacccatacgatgttccagattacgcttaacaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacggggtaccccgtactagagcggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcg BBa_K1897008_sequence 1 ttgacagctagctcagtcctaggtataatgctagcattaaagaggagaaaccggtcgaccggatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattacccatacgatgttccagattacgcttaacaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacggggtaccccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z