BBa_K1897018
type: ComponentDefinition
persistent identity: BBa K1897018
display id: BBa_K1897018
version: 1
was generated by: iGEM to SBOL conversion
description: LuxR + LuxI + Invasin
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T01:41:32Z
mutable provenance: LuxR and LuxI are derived from Vibrio fischeri, while Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see <partinfo>BBa_K1897010</partinfo>.
owned by: james
owned by: myers
mutable description: This part is made by 3A ligation of LuxR <partinfo>BBa_K1897008</partinfo>, LuxI <partinfo>BBa_K1897009</partinfo> and Invasin <partinfo>BBa_K1897011</partinfo>.
discontinued: false
dominant: false
group u list: _2363_
m user id: 29432
owner id: 29432
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot, and two HA tag for Invasin protein.
star: false
creator: Ang Shi Hui
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1897011
component: BBa_K1897015
sequence annotation: BBa_K1897015
sequence annotation: BBa_K1897011
sequence: https://synbiohub.org/public/igem/BBa_K1897018_sequence/1

BBa_K1897011
type: Component
persistent identity: component2491275
display id: component2491275
version: 1
top level: BBa_K1897018
definition: BBa_K1897011
access: public

BBa_K1897015
type: Component
persistent identity: component2491267
display id: component2491267
version: 1
top level: BBa_K1897018
definition: BBa_K1897015
access: public

BBa_K1897015
type: SequenceAnnotation
persistent identity: annotation2491267
display id: annotation2491267
version: 1
top level: BBa_K1897018
location: https://synbiohub.org/public/igem/BBa_K1897018/annotation2491267/range2491267/1
component: BBa_K1897015

https://synbiohub.org/public/igem/BBa_K1897018/annotation2491267/range2491267/1
type: Range
persistent identity: range2491267
display id: range2491267
version: 1
top level: BBa_K1897018
direction: direction/reverse
start: 1
end: 1860
orientation: inline

BBa_K1897011
type: SequenceAnnotation
persistent identity: annotation2491275
display id: annotation2491275
version: 1
top level: BBa_K1897018
location: https://synbiohub.org/public/igem/BBa_K1897018/annotation2491275/range2491275/1
component: BBa_K1897011

https://synbiohub.org/public/igem/BBa_K1897018/annotation2491275/range2491275/1
type: Range
persistent identity: range2491275
display id: range2491275
version: 1
top level: BBa_K1897018
direction: direction/reverse
start: 1869
end: 5122
orientation: inline

BBa_K1897011
type: ComponentDefinition
persistent identity: BBa K1897011
display id: BBa_K1897011
version: 1
was generated by: iGEM to SBOL conversion
description: Invasin
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T12:54:39Z
mutable provenance: Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see <partinfo>BBa K1897010</partinfo>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
owned by: james
owned by: myers
mutable description: This part was made up of promoter pLuxR </partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, Invasin coding sequence <partinfo>BBa_K1897010</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
discontinued: false
dominant: false
group u list: _2363_
m user id: 29432
owner id: 29432
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS <partinfo>BBa_K1897010</partinfo> is shifted to after the HA tag.
star: false
creator: Ang Shi Hui
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_R0062
sequence annotation: BBa_B0030
sequence annotation: BBa_K1897010
sequence annotation: HA tag
sequence annotation: Stop Codon
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1897011_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2491158
display id: annotation2491158
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491158/range2491158/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491158/range2491158/1
type: Range
persistent identity: range2491158
display id: range2491158
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 13
end: 67
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2491159
display id: annotation2491159
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491159/range2491159/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491159/range2491159/1
type: Range
persistent identity: range2491159
display id: range2491159
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 68
end: 82
orientation: inline

BBa_K1897010
type: SequenceAnnotation
persistent identity: annotation2491160
display id: annotation2491160
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491160/range2491160/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491160/range2491160/1
type: Range
persistent identity: range2491160
display id: range2491160
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 91
end: 3048
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2491161
display id: annotation2491161
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491161/range2491161/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491161/range2491161/1
type: Range
persistent identity: range2491161
display id: range2491161
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 3049
end: 3075
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2491162
display id: annotation2491162
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491162/range2491162/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491162/range2491162/1
type: Range
persistent identity: range2491162
display id: range2491162
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 3076
end: 3102
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2491163
display id: annotation2491163
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491163/range2491163/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491163/range2491163/1
type: Range
persistent identity: range2491163
display id: range2491163
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 3103
end: 3105
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2491164
display id: annotation2491164
version: 1
top level: BBa_K1897011
location: https://synbiohub.org/public/igem/BBa_K1897011/annotation2491164/range2491164/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897011/annotation2491164/range2491164/1
type: Range
persistent identity: range2491164
display id: range2491164
version: 1
top level: BBa_K1897011
direction: direction/forward
start: 3106
end: 3234
orientation: inline

BBa_K1897015
type: ComponentDefinition
persistent identity: BBa K1897015
display id: BBa_K1897015
version: 1
was generated by: iGEM to SBOL conversion
description: LuxR + LuxI
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T01:31:43Z
mutable provenance: These parts are derived from Vibrio fischeri.
owned by: james
owned by: myers
mutable description: This part is made by 3A ligation of LuxR <partinfo>BBa_K1897008</partinfo> and LuxI <partinfo>BBa_K1897009</partinfo>.
discontinued: false
dominant: false
group u list: _2363_
m user id: 29432
owner id: 29432
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot.
star: false
creator: Ang Shi Hui
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1897009
component: BBa_K1897008
sequence annotation: BBa_K1897008
sequence annotation: BBa_K1897009
sequence: https://synbiohub.org/public/igem/BBa_K1897015_sequence/1

BBa_K1897009
type: Component
persistent identity: component2491207
display id: component2491207
version: 1
top level: BBa_K1897015
definition: BBa_K1897009
access: public

BBa_K1897008
type: Component
persistent identity: component2491200
display id: component2491200
version: 1
top level: BBa_K1897015
definition: BBa_K1897008
access: public

BBa_K1897008
type: SequenceAnnotation
persistent identity: annotation2491200
display id: annotation2491200
version: 1
top level: BBa_K1897015
location: https://synbiohub.org/public/igem/BBa_K1897015/annotation2491200/range2491200/1
component: BBa_K1897008

https://synbiohub.org/public/igem/BBa_K1897015/annotation2491200/range2491200/1
type: Range
persistent identity: range2491200
display id: range2491200
version: 1
top level: BBa_K1897015
direction: direction/reverse
start: 1
end: 975
orientation: inline

BBa_K1897009
type: SequenceAnnotation
persistent identity: annotation2491207
display id: annotation2491207
version: 1
top level: BBa_K1897015
location: https://synbiohub.org/public/igem/BBa_K1897015/annotation2491207/range2491207/1
component: BBa_K1897009

https://synbiohub.org/public/igem/BBa_K1897015/annotation2491207/range2491207/1
type: Range
persistent identity: range2491207
display id: range2491207
version: 1
top level: BBa_K1897015
direction: direction/reverse
start: 984
end: 1860
orientation: inline

BBa_K1897008
type: ComponentDefinition
persistent identity: BBa K1897008
display id: BBa_K1897008
version: 1
was generated by: iGEM to SBOL conversion
description: LuxR
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T11:16:42Z
mutable provenance: This part was made based on the genome of Vibrio fischeri.
owned by: james
owned by: myers
mutable description: This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015. This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI. This also produces light due to the production of LuxCDABE. Transcription of operonL leads to the production of LuxR. In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon. This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). It also consists of an enzyme cut site XbaI after the biobricks prefix and a KpnI site before the biobricks suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _2363_
m user id: 29432
owner id: 29432
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is also one HA tag available for characterisation of the protein produced via western blot.
star: false
creator: Ang Shi Hui
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_J23119
sequence annotation: BBa_B0030
sequence annotation: BBa_C0062
sequence annotation: HA tag
sequence annotation: Stop Codon
sequence annotation: rrnBT1
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1897008_sequence/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2491075
display id: annotation2491075
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491075/range2491075/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491075/range2491075/1
type: Range
persistent identity: range2491075
display id: range2491075
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2491076
display id: annotation2491076
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491076/range2491076/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491076/range2491076/1
type: Range
persistent identity: range2491076
display id: range2491076
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 36
end: 50
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2491077
display id: annotation2491077
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491077/range2491077/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491077/range2491077/1
type: Range
persistent identity: range2491077
display id: range2491077
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 63
end: 812
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2491078
display id: annotation2491078
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491078/range2491078/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491078/range2491078/1
type: Range
persistent identity: range2491078
display id: range2491078
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 813
end: 839
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2491079
display id: annotation2491079
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491079/range2491079/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491079/range2491079/1
type: Range
persistent identity: range2491079
display id: range2491079
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 840
end: 842
orientation: inline

rrnBT1
type: SequenceAnnotation
persistent identity: annotation2491080
display id: annotation2491080
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491080/range2491080/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491080/range2491080/1
type: Range
persistent identity: range2491080
display id: range2491080
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 843
end: 914
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2491081
display id: annotation2491081
version: 1
top level: BBa_K1897008
location: https://synbiohub.org/public/igem/BBa_K1897008/annotation2491081/range2491081/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1897008/annotation2491081/range2491081/1
type: Range
persistent identity: range2491081
display id: range2491081
version: 1
top level: BBa_K1897008
direction: direction/forward
start: 923
end: 963
orientation: inline

BBa_K1897009
type: ComponentDefinition
persistent identity: BBa K1897009
display id: BBa_K1897009
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI
created: 2016-10-09T11:00:00Z
modified: 2016-10-10T11:32:29Z
mutable provenance: LuxI was obtained from biobrick part <partinfo>BBa_C0061</partinfo>, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
owned by: james
owned by: myers
mutable description: This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, LuxI coding sequence <partinfo>BBa_C0061</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
discontinued: false
dominant: false
group u list: _2363_
m user id: 29432
owner id: 29432
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag.
star: false
creator: Ang Shi Hui
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_R0062
sequence annotation: BBa_B0030
sequence annotation: BBa_C0061
sequence annotation: HA tag
sequence annotation: Stop Codon
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1897009_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2491093
display id: annotation2491093
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491093/range2491093/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491093/range2491093/1
type: Range
persistent identity: range2491093
display id: range2491093
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 13
end: 67
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2491094
display id: annotation2491094
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491094/range2491094/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491094/range2491094/1
type: Range
persistent identity: range2491094
display id: range2491094
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 68
end: 82
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2491095
display id: annotation2491095
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491095/range2491095/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491095/range2491095/1
type: Range
persistent identity: range2491095
display id: range2491095
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 95
end: 706
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2491097
display id: annotation2491097
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491097/range2491097/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491097/range2491097/1
type: Range
persistent identity: range2491097
display id: range2491097
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 707
end: 733
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2491098
display id: annotation2491098
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491098/range2491098/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491098/range2491098/1
type: Range
persistent identity: range2491098
display id: range2491098
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 734
end: 736
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2491096
display id: annotation2491096
version: 1
top level: BBa_K1897009
location: https://synbiohub.org/public/igem/BBa_K1897009/annotation2491096/range2491096/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1897009/annotation2491096/range2491096/1
type: Range
persistent identity: range2491096
display id: range2491096
version: 1
top level: BBa_K1897009
direction: direction/forward
start: 737
end: 865
orientation: inline

https://synbiohub.org/public/igem/BBa_K1897018_sequence/1
type: Sequence
persistent identity: BBa K1897018 sequence
display id: BBa_K1897018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagcattaaagaggagaaaccggtcgaccggatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattacccatacgatgttccagattacgcttaacaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacggggtaccccgtactagagcggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcgtactagagcgcggatccgcgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaggtcgaccatgatggttttccagccaatcagtgagtttctcttgataagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1897011_sequence/1
type: Sequence
persistent identity: BBa K1897011 sequence
display id: BBa_K1897011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcggatccgcgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaggtcgaccatgatggttttccagccaatcagtgagtttctcttgataaggaatgcgggaatgtctatgtattttaataaaataatttcatttaatattatttcacgaatagttatttgtatctttttgatatgtggaatgttcatggctggggcttcagaaaaatatgatgctaacgcaccgcaacaggtccagccttattctgtctcttcatctgcatttgaaaatctccatcctaataatgaaatggagagttcaatcaatcccttttccgcatcggatacagaaagaaatgctgcaataatagatcgcgccaataaggagcaggagactgaagcggtgaataagatgataagcaccggggccaggttagctgcatcaggcagggcatctgatgttgctcactcaatggtgggcgatgcggttaatcaagaaatcaaacagtggttaaatcgattcggtacggctcaagttaatctgaattttgacaaaaatttttcgctaaaagaaagctctcttgattggctggctccttggtatgactctgcttcattcctcttttttagtcagttaggtattcgcaataaagacagccgcaacacacttaaccttggcgtcgggatacgtacattggagaacggttggctgtacggacttaatactttttatgataatgatttgaccggccacaaccaccgtatcggtcttggtgccgaggcctggaccgattatttacagttggctgccaatgggtattttcgcctcaatggatggcactcgtcgcgtgatttctccgactataaagagcgcccagccactgggggggatttgcgcgcgaatgcttatttacctgcactcccacaactgggggggaagttgatgtatgagcaatacaccggtgagcgtgttgctttatttggtaaagataatctgcaacgcaacccttatgccgtgactgccgggatcaattacacccccgtgcctctactcactgtcggggtagatcagcgtatggggaaaagcagtaagcatgaaacacagtggaacctccaaatgaactatcgcctgggcgagagttttcagtcgcaacttagcccttcagcggtggcaggaacacgtctactggcggagagccgctataaccttgtcgatcgtaacaataatatcgtgttggagtatcagaaacagcaggtggttaaactgacattatcgccagcaactatctccggcctgccgggtcaggtttatcaggtgaacgcacaagtacaaggggcatctgctgtaagggaaattgtctggagtgatgccgaactgattgccgctggcggcacattaacaccactgagtaccacacaattcaacttggttttaccgccttataaacgcacagcacaagtgagtcgggtaacggacgacctgacagccaacttttattcgcttagtgcgctcgcggttgatcaccaaggaaaccgatctaactcattcacattgagcgtcaccgttcagcagcctcagttgacattaacggcggccgtcattggtgatggcgcaccggctaatgggaaaactgcaatcaccgttgagttcaccgttgctgattttgaggggaaacccttagccgggcaggaggtggtgataaccaccaataatggtgcgctaccgaataaaatcacggaaaagacagatgcaaatggcgtcgcgcgcattgcattaaccaatacgacagatggcgtgacggtagtcacagcagaagtggaggggcaacggcaaagtgttgatacccactttgttaagggtactatcgcggcggataaatccactctggctgcggtaccgacatctatcatcgctgatggtctaatggcttcaaccatcacgttggagttgaaggatacctatggggacccgcaggctggcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1897015_sequence/1
type: Sequence
persistent identity: BBa K1897015 sequence
display id: BBa_K1897015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagcattaaagaggagaaaccggtcgaccggatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattacccatacgatgttccagattacgcttaacaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacggggtaccccgtactagagcggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1897008_sequence/1
type: Sequence
persistent identity: BBa K1897008 sequence
display id: BBa_K1897008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagcattaaagaggagaaaccggtcgaccggatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattacccatacgatgttccagattacgcttaacaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacggggtaccccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1897009_sequence/1
type: Sequence
persistent identity: BBa K1897009 sequence
display id: BBa_K1897009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggggtaccccgacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaaattaaagaggagaaaccggtcgaccggatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttacccatacgatgttccagattacgcttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacgcggatccgcg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z