LuxR and LuxI are derived from Vibrio fischeri, while Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see <partinfo>BBa_K1897010</partinfo>.
This part is made by 3A ligation of LuxR <partinfo>BBa_K1897008</partinfo>, LuxI <partinfo>BBa_K1897009</partinfo> and Invasin <partinfo>BBa_K1897011</partinfo>.
There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot, and two HA tag for Invasin protein.
Invasin is derived from Yersinia pseudotuberculosis, from the coding sequence [https://www.ncbi.nlm.nih.gov/nuccore/M17448.1 [Genbank M17448.1]] (see <partinfo>BBa K1897010</partinfo>. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
This part was made up of promoter pLuxR </partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, Invasin coding sequence <partinfo>BBa_K1897010</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for Invasin CDS <partinfo>BBa_K1897010</partinfo> is shifted to after the HA tag.
These parts are derived from Vibrio fischeri.
This part is made by 3A ligation of LuxR <partinfo>BBa_K1897008</partinfo> and LuxI <partinfo>BBa_K1897009</partinfo>.
There is one HA tag available for characterisation of the LuxR and LuxI proteins produced via western blot.
This part was made based on the genome of Vibrio fischeri.
This part was made up of promoter BBa_J23119, RBS BBa_B0030, LuxR coding sequence BBa_C0062 and double terminator BBa_B0015. This part allows for the constitutive expression of LuxR protein from the promoter BBa_J23119. The LuxR protein originated from Vibrio fischeri, and is a regulatory protein of the Lux operon. The Lux operon can be transcribed from from te left (operonL) or right (operonR). Transcription of operonR, which consists of LuxICDABE, occurs only in the presence of N-(3-oxo-hexanoyl)-homoserine lactone (AHL), which is an autoinducer produced by LuxI. This also produces light due to the production of LuxCDABE. Transcription of operonL leads to the production of LuxR. In the presence of rising amounts of AHL, LuxR-AHL complexes begin to form, and LuxR undergoes a conformational change that leads to the activation of the Lux operon. This part can be used if you need constitutive production of LuxR to activate production of genes under the control of pLuxR (BBa_R0062). It also consists of an enzyme cut site XbaI after the biobricks prefix and a KpnI site before the biobricks suffix.
There is also one HA tag available for characterisation of the protein produced via western blot.
LuxI was obtained from biobrick part <partinfo>BBa_C0061</partinfo>, which was derived from Vibrio fischeri. The promoter was synthesized based on the sequence obtained from <partinfo>BBa_R0062</partinfo>. The ribosome binding site (RBS) was synthesized based on the sequence obtained from <partinfo>BBa_B0030</partinfo>. The transcription terminators were synthesized based on the sequence obtained from <partinfo>BBa_B0015</partinfo>.
This part was made up of promoter pLuxR <partinfo>BBa_R0062</partinfo>, ribosome binding site (RBS) <partinfo>BBa_B0030</partinfo>, LuxI coding sequence <partinfo>BBa_C0061</partinfo> and double terminator <partinfo>BBa_B0015</partinfo>.
There is one HA tag available for characterisation of the protein produced via western blot. Note that the stop codon for LuxI BBa_C0061 is shifted to after the HA tag.
Conversion of the iGEM parts registry to SBOL2.1