BBa_K1172901 1 BBa_K1172901 Alanine racemase from ''E. coli'' 2013-08-25T11:00:00Z 2015-05-08T01:09:35Z E. coli KRX genomic DNA Alanine-racemase extracted from genomic DNA from E. coli KRX. Used for our biosafety project. false false _1485_ 0 16785 9 In stock true Ligation via Gibson-assembly, so the primer had to have certain extensions false Tore Bleckwehl annotation2357203 1 K1172901 (Alanine-Racemase) range2357203 1 1 1080 BBa_K1897038 1 BBa_K1897038 Scar site generated by 3A Assembly 2016-10-13T11:00:00Z 2016-10-14T07:07:57Z Synthetic linker Scar site generated by 3A Assembly of Part BBa_K314100 and Part BBa_K1172901 false false _2363_ 29442 29442 9 false nil false Nguyen Hoang Diem Phuong BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1847008 1 BBa_K1847008 lldRO1-J23117-lldRO2 2015-08-25T11:00:00Z 2015-09-17T08:54:56Z Synthetic synthesis Collection of promoters regulated by LldR. false false _2273_ 26442 26442 9 false false Anna Fomitcheva Khartchenko annotation2438077 1 lldRO2 range2438077 1 127 143 annotation2438076 1 J23117 range2438076 1 72 106 annotation2438075 1 lldRO1 range2438075 1 1 17 BBa_K1897030 1 BBa_K1897030 Lambda t0 terminator 2016-10-12T11:00:00Z 2016-10-13T07:53:33Z Source Lambda t0 terminator false false _2363_ 29424 29424 9 false Considerations false Keshiniy Madivannan BBa_K1897019 1 BBa_K1897019 p70-34-ALR-Terminator 2016-10-10T11:00:00Z 2016-10-14T12:07:41Z Source The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (BBa_K1847008) with a strong RBS (BBa_B0034), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator. false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan component2508216 1 BBa_B0034 component2508217 1 BBa_K1897038 component2508220 1 BBa_M36117 component2508213 1 BBa_K1847008 component2508214 1 BBa_K1897039 component2508219 1 BBa_K1172901 component2508221 1 BBa_K1897030 annotation2508220 1 BBa_M36117 range2508220 1 1250 1252 annotation2508216 1 BBa_B0034 range2508216 1 152 163 annotation2508219 1 BBa_K1172901 range2508219 1 170 1249 annotation2508214 1 BBa_K1897039 range2508214 1 144 151 annotation2508217 1 BBa_K1897038 range2508217 1 164 169 annotation2508213 1 BBa_K1847008 range2508213 1 1 143 annotation2508221 1 BBa_K1897030 range2508221 1 1253 1357 BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_K1897039 1 BBa_K1897039 Scar site generated by 3A Assembly 2016-10-13T11:00:00Z 2016-10-14T10:53:46Z Synthetic linker Scar site generated by 3A Assembly false false _2363_ 29442 29442 9 false nil false Nguyen Hoang Diem Phuong BBa_K1172901_sequence 1 atgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaa BBa_K1897039_sequence 1 tactagag BBa_K1897038_sequence 1 tactag BBa_K1897019_sequence 1 aattggccctaccaattcttcgcttatctgacctctggttcacaatttcccaattaaaactcacatcaatgttgacagctagctcagtcctagggattgtgctagctcattatccctacacaacacaattggcagtgccactttactagagaaagaggagaaatactagatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaataaagtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_M36117_sequence 1 taa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1897030_sequence 1 agtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_K1847008_sequence 1 aattggccctaccaattcttcgcttatctgacctctggttcacaatttcccaattaaaactcacatcaatgttgacagctagctcagtcctagggattgtgctagctcattatccctacacaacacaattggcagtgccactt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z