BBa_K1897034 1 BBa_K1897034 B0032-Scar site 2016-10-12T11:00:00Z 2016-10-14T12:38:00Z Source B0032 variant for constructs with J23100 promoter false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan annotation2508664 1 Scar range2508664 1 14 19 BBa_K1172901 1 BBa_K1172901 Alanine racemase from ''E. coli'' 2013-08-25T11:00:00Z 2015-05-08T01:09:35Z E. coli KRX genomic DNA Alanine-racemase extracted from genomic DNA from E. coli KRX. Used for our biosafety project. false false _1485_ 0 16785 9 In stock true Ligation via Gibson-assembly, so the primer had to have certain extensions false Tore Bleckwehl annotation2357203 1 K1172901 (Alanine-Racemase) range2357203 1 1 1080 BBa_K1897030 1 BBa_K1897030 Lambda t0 terminator 2016-10-12T11:00:00Z 2016-10-13T07:53:33Z Source Lambda t0 terminator false false _2363_ 29424 29424 9 false Considerations false Keshiniy Madivannan BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_K1897035 1 BBa_K1897035 High constitutive expression cassette without RBS 2016-10-12T11:00:00Z 2016-10-14T12:23:51Z Source Promoter J23100 without B0034 RBS. false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan annotation2508624 1 f1 origin K314110 range2508624 1 1 455 annotation2508657 1 J23100 range2508657 1 464 498 BBa_K1897026 1 BBa_K1897026 J23100-32-ALR-Terminator 2016-10-10T11:00:00Z 2016-10-14T08:27:47Z Source The construct contains a high expression promoter J23100 in a cassette (BBa_K314100) with a medium RBS (BBa_B0032), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator. false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan component2505978 1 BBa_M36117 component2505979 1 BBa_K1897030 component2505974 1 BBa_K1897035 component2505977 1 BBa_K1172901 component2505975 1 BBa_K1897034 annotation2505979 1 BBa_K1897030 range2505979 1 1609 1713 annotation2505974 1 BBa_K1897035 range2505974 1 1 506 annotation2505977 1 BBa_K1172901 range2505977 1 526 1605 annotation2505978 1 BBa_M36117 range2505978 1 1606 1608 annotation2505975 1 BBa_K1897034 range2505975 1 507 525 BBa_K1172901_sequence 1 atgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaa BBa_K1897034_sequence 1 tcacacaggaaagtactag BBa_M36117_sequence 1 taa BBa_K1897030_sequence 1 agtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_K1897035_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagag BBa_K1897026_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaataaagtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z