BBa_K1172901 1 BBa_K1172901 Alanine racemase from ''E. coli'' 2013-08-25T11:00:00Z 2015-05-08T01:09:35Z E. coli KRX genomic DNA Alanine-racemase extracted from genomic DNA from E. coli KRX. Used for our biosafety project. false false _1485_ 0 16785 9 In stock true Ligation via Gibson-assembly, so the primer had to have certain extensions false Tore Bleckwehl annotation2357203 1 K1172901 (Alanine-Racemase) range2357203 1 1 1080 BBa_K1897027 1 BBa_K1897027 J23100-33-ALR-Terminator 2016-10-10T11:00:00Z 2016-10-14T08:33:09Z Source The construct contains a high expression promoter J23100 in a cassette (BBa_K314100) with a weaker RBS (BBa_B0033), followed by a CDS of ALR (BBa_K1172901) and a lambda t0 terminator. false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan component2506006 1 BBa_K1897030 component2506002 1 BBa_K1897036 component2506005 1 BBa_M36117 component2506001 1 BBa_K1897035 component2506004 1 BBa_K1172901 annotation2506005 1 BBa_M36117 range2506005 1 1604 1606 annotation2506006 1 BBa_K1897030 range2506006 1 1607 1711 annotation2506001 1 BBa_K1897035 range2506001 1 1 506 annotation2506002 1 BBa_K1897036 range2506002 1 507 523 annotation2506004 1 BBa_K1172901 range2506004 1 524 1603 BBa_K1897030 1 BBa_K1897030 Lambda t0 terminator 2016-10-12T11:00:00Z 2016-10-13T07:53:33Z Source Lambda t0 terminator false false _2363_ 29424 29424 9 false Considerations false Keshiniy Madivannan BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_K1897036 1 BBa_K1897036 B0033-Scar site 2016-10-12T11:00:00Z 2016-10-14T12:43:12Z Source B0033 Mixed RE site for J23100-B0033 sequence false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan annotation2508791 1 Scar range2508791 1 12 17 BBa_K1897035 1 BBa_K1897035 High constitutive expression cassette without RBS 2016-10-12T11:00:00Z 2016-10-14T12:23:51Z Source Promoter J23100 without B0034 RBS. false false _2363_ 29442 29424 9 false Considerations false Keshiniy Madivannan annotation2508657 1 J23100 range2508657 1 464 498 annotation2508624 1 f1 origin K314110 range2508624 1 1 455 BBa_K1172901_sequence 1 atgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaa BBa_K1897036_sequence 1 tcacacaggactactag BBa_M36117_sequence 1 taa BBa_K1897030_sequence 1 agtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat BBa_K1897035_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagag BBa_K1897027_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggactactagatgcaagcggcaactgttgtgattaaccgccgcgctctgcgacacaacctgcaacgtcttcgtgaactggcccctgccagtaaaatggttgcggtggtgaaagcgaacgcttatggtcacggtcttcttgagaccgcgcgaacgctccccgatgctgacgcctttggcgtagcccgtctcgaagaagctctgcgactgcgtgcggggggaatcaccaaacctgtactgttactcgaaggcttttttgatgccagagatctgccgacgatttctgcgcaacattttcataccgccgtgcataacgaagaacagctggctgcgctggaagaggctagcctggacgagccggttaccgtctggatgaaactcgataccggtatgcaccgtctgggcgtaaggccggaacaggctgaggcgttttatcatcgcctgacccagtgcaaaaacgttcgtcagccggtgaatatcgtcagccattttgcgcgcgcggatgaaccaaaatgtggcgcaaccgagaaacaactcgctatctttaataccttttgcgaaggcaaacctggtcaacgttccattgccgcgtcgggtggcattctgctgtggccacagtcgcattttgactgggtgcgcccgggcatcattctttatggcgtctcgccgctggaagatcgctccaccggtgccgattttggctgtcagccagtgatgtcactaacctccagcctgattgccgtgcgtgagcataaagccggagagcctgttggttatggtggaacctgggtaagcgaacgtgatacccgtcttggcgtagtcgcgatgggctatggcgatggttatccgcgcgccgcgccgtccggtacgccagtgctggtgaacggtcgcgaagtaccgattgtcgggcgcgtggcgatggatatgatctgcgtagacttaggtccacaggcgcaggacaaagccggggatccggtcattttatggggcgaaggtttgcccgtagaacgtatcgctgaaatgacgaaagtaagcgcttacgaacttattacgcgcctgacttcaagggtcgcgatgaaatacgtggattaataaagtagtcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z