LOCUS       BBa_K1898150            1058 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong promoter + Strong RBS + CH25H + 10x His tag + Double 
            terminator
ACCESSION   BBa_K1898150
VERSION     BBa_K1898150.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..877
                     /label=BBa_K1898100
     misc_feature    886..915
                     /label=10x-Histidine Tag
     misc_feature    916..918
                     /label=Stop
     misc_feature    919..921
                     /label=Stop
     misc_feature    886..921
                     /label=BBa_K844000
     stem_loop       941..984
                     /label=stem_loop
     misc_feature    930..1009
                     /label=BBa_B0010
     terminator      930..1009
                     /label=BBa_B0010
     stem_loop       1025..1044
                     /label=T7 TE
     misc_feature    1051..1051
                     /label=stop
     polyA_site      1045..1058
                     /label=polya
     misc_feature    1018..1058
                     /label=BBa_B0012
     terminator      1018..1058
                     /label=BBa_B0012
     terminator      930..1058
                     /label=BBa_B0015
     misc_feature    886..1058
                     /label=BBa_S05336
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagctgc cacaactgct ccgaccccca ggtcctttgc agctccgggc agctgttcct
      121 acagcccctc tgggaccacc tgaggagctg ggaggccctc ctacagtcgc ccttcttccc
      181 ggtcatcttc tccatcacca catacgtggg cttttgcctg cccttcgtgg tcctggatat
      241 cctgtgctcc tgggtgcccg ccctgcggcg ctacaagatc caccctgact tctcgccatc
      301 cgcgcagcag ctgctacctt gcctggggca gaccctctac cagcatgtga tgtttgtgtt
      361 ccccgtgacg ctgctgcatt gggcccgcag cccggccctc ctgccccacg aagctcccga
      421 gctgctcctg ctgctgcacc acatcctgtt ctgcctgcta ctcttcgaca tggagttctt
      481 cgtgtggcac ctgctgcacc acaaggtgcc ctggctgtac cgcaccttcc acaaggtgca
      541 ccaccagaac tcgtcctcgt tcgcgctggc aacgcagtat atgagcgtct gggaactgtt
      601 ttctttgggc ttcttcgaca tgatgaacgt cacactgctc gggtgccacc cgctcaccac
      661 cctgaccttc cacgtggtca acatctggct ttccgtggag gaccactccg gctacaactt
      721 cccttggtcc actcacagac tggtgccctt cgggtggtac gggggtgtgg tgcaccacga
      781 cctgcatcac tctcacttta actgcaactt cgctccgtac tttacacact gggacaaaat
      841 actgggaacg ctgcggactg catctgtccc agcgcggtac tagagcatca tcaccatcac
      901 caccatcatc accattaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      961 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1021 cactggctca ccttcgggtg ggcctttctg cgtttata
//