BBa_K1898150
type: ComponentDefinition
persistent identity: BBa K1898150
display id: BBa_K1898150
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator
created: 2016-10-09T11:00:00Z
modified: 2016-10-19T09:44:01Z
mutable provenance: this DNA is synthesized by IDT.
owned by: james
owned by: myers
mutable description: BBa_K880005 consists of strong promoter and strong rbs to maximize protein production. This construct codes for ch25h, a catalyst for the conversion of cholesterol to 25 hydroxycholesterol, and 10x histidine tag for protein purification. BBa__B0015 is a double terminator used to stop transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _2364_
m user id: 25587
owner id: 25587
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA.
star: false
creator: Fiona Tsai
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1898100
component: BBa_K880005
component: BBa_S05336
sequence annotation: BBa_K880005
sequence annotation: BBa_K1898100
sequence annotation: BBa_S05336
sequence: https://synbiohub.org/public/igem/BBa_K1898150_sequence/1

BBa_K1898100
type: Component
persistent identity: component2524346
display id: component2524346
version: 1
top level: BBa_K1898150
definition: BBa_K1898100
access: public

BBa_K880005
type: Component
persistent identity: component2524345
display id: component2524345
version: 1
top level: BBa_K1898150
definition: BBa_K880005
access: public

BBa_S05336
type: Component
persistent identity: component2524358
display id: component2524358
version: 1
top level: BBa_K1898150
definition: BBa_S05336
access: public

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2524345
display id: annotation2524345
version: 1
top level: BBa_K1898150
location: https://synbiohub.org/public/igem/BBa_K1898150/annotation2524345/range2524345/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1898150/annotation2524345/range2524345/1
type: Range
persistent identity: range2524345
display id: range2524345
version: 1
top level: BBa_K1898150
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_K1898100
type: SequenceAnnotation
persistent identity: annotation2524346
display id: annotation2524346
version: 1
top level: BBa_K1898150
location: https://synbiohub.org/public/igem/BBa_K1898150/annotation2524346/range2524346/1
component: BBa_K1898100

https://synbiohub.org/public/igem/BBa_K1898150/annotation2524346/range2524346/1
type: Range
persistent identity: range2524346
display id: range2524346
version: 1
top level: BBa_K1898150
direction: direction/reverse
start: 62
end: 877
orientation: inline

BBa_S05336
type: SequenceAnnotation
persistent identity: annotation2524358
display id: annotation2524358
version: 1
top level: BBa_K1898150
location: https://synbiohub.org/public/igem/BBa_K1898150/annotation2524358/range2524358/1
component: BBa_S05336

https://synbiohub.org/public/igem/BBa_K1898150/annotation2524358/range2524358/1
type: Range
persistent identity: range2524358
display id: range2524358
version: 1
top level: BBa_K1898150
direction: direction/reverse
start: 886
end: 1058
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1898100
type: ComponentDefinition
persistent identity: BBa K1898100
display id: BBa_K1898100
version: 1
was generated by: iGEM to SBOL conversion
description: ch25h, cholesterol 25 hydroxylase
created: 2016-10-09T11:00:00Z
modified: 2016-10-16T12:36:44Z
mutable provenance: cDNA of ch25h was ordered from Sino BIological Inc
owned by: james
owned by: myers
mutable description: This DNA codes for ch25h, cholesterol 25-hydroxylase, that catalyzes the formation of 25 hydroxycholesterol from cholesterol. 25 hydroxycholesterol has been reported to have a range of effects on the immune system, including the suppression of production of IgA by B cells.
discontinued: false
dominant: false
experience: experience/None
group u list: _2364_
m user id: 25587
owner id: 25587
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We removed three point mutations from the cDNA with mutagenesis done by MIssionBiotech.
star: false
creator: Fiona Tsai
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1898100_sequence/1

BBa_S05336
type: ComponentDefinition
persistent identity: BBa S05336
display id: BBa_S05336
version: 1
was generated by: iGEM to SBOL conversion
description: K844000:B0015
created: 2016-10-09T11:00:00Z
modified: 2016-10-19T09:39:35Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 25587
owner id: 25587
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Fiona Tsai
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0015
component: BBa_K844000
sequence annotation: BBa_K844000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S05336_sequence/1

BBa_B0015
type: Component
persistent identity: component2524341
display id: component2524341
version: 1
top level: BBa_S05336
definition: BBa_B0015
access: public

BBa_K844000
type: Component
persistent identity: component2524334
display id: component2524334
version: 1
top level: BBa_S05336
definition: BBa_K844000
access: public

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2524334
display id: annotation2524334
version: 1
top level: BBa_S05336
location: https://synbiohub.org/public/igem/BBa_S05336/annotation2524334/range2524334/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_S05336/annotation2524334/range2524334/1
type: Range
persistent identity: range2524334
display id: range2524334
version: 1
top level: BBa_S05336
direction: direction/reverse
start: 1
end: 36
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2524341
display id: annotation2524341
version: 1
top level: BBa_S05336
location: https://synbiohub.org/public/igem/BBa_S05336/annotation2524341/range2524341/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05336/annotation2524341/range2524341/1
type: Range
persistent identity: range2524341
display id: range2524341
version: 1
top level: BBa_S05336
direction: direction/reverse
start: 45
end: 173
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K844000
type: ComponentDefinition
persistent identity: BBa K844000
display id: BBa_K844000
version: 1
was generated by: iGEM to SBOL conversion
description: 10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:33Z
mutable provenance: Constructed through oligonucleotide annealing
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 0
owner id: 9404
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Kathleen Miller
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: 10x-Histidine Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K844000_sequence/1

10x-Histidine Tag
type: SequenceAnnotation
persistent identity: annotation2206607
display id: annotation2206607
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K844000/annotation2206607/range2206607/1
type: Range
persistent identity: range2206607
display id: range2206607
version: 1
top level: BBa_K844000
direction: direction/forward
start: 1
end: 30
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206608
display id: annotation2206608
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206608/range2206608/1
type: Range
persistent identity: range2206608
display id: range2206608
version: 1
top level: BBa_K844000
direction: direction/forward
start: 31
end: 33
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2206609
display id: annotation2206609
version: 1
top level: BBa_K844000
location: https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K844000/annotation2206609/range2206609/1
type: Range
persistent identity: range2206609
display id: range2206609
version: 1
top level: BBa_K844000
direction: direction/forward
start: 34
end: 36
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1898150_sequence/1
type: Sequence
persistent identity: BBa K1898150 sequence
display id: BBa_K1898150_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagctgccacaactgctccgacccccaggtcctttgcagctccgggcagctgttcctacagcccctctgggaccacctgaggagctgggaggccctcctacagtcgcccttcttcccggtcatcttctccatcaccacatacgtgggcttttgcctgcccttcgtggtcctggatatcctgtgctcctgggtgcccgccctgcggcgctacaagatccaccctgacttctcgccatccgcgcagcagctgctaccttgcctggggcagaccctctaccagcatgtgatgtttgtgttccccgtgacgctgctgcattgggcccgcagcccggccctcctgccccacgaagctcccgagctgctcctgctgctgcaccacatcctgttctgcctgctactcttcgacatggagttcttcgtgtggcacctgctgcaccacaaggtgccctggctgtaccgcaccttccacaaggtgcaccaccagaactcgtcctcgttcgcgctggcaacgcagtatatgagcgtctgggaactgttttctttgggcttcttcgacatgatgaacgtcacactgctcgggtgccacccgctcaccaccctgaccttccacgtggtcaacatctggctttccgtggaggaccactccggctacaacttcccttggtccactcacagactggtgcccttcgggtggtacgggggtgtggtgcaccacgacctgcatcactctcactttaactgcaacttcgctccgtactttacacactgggacaaaatactgggaacgctgcggactgcatctgtcccagcgcggtactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1898100_sequence/1
type: Sequence
persistent identity: BBa K1898100 sequence
display id: BBa_K1898100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagctgccacaactgctccgacccccaggtcctttgcagctccgggcagctgttcctacagcccctctgggaccacctgaggagctgggaggccctcctacagtcgcccttcttcccggtcatcttctccatcaccacatacgtgggcttttgcctgcccttcgtggtcctggatatcctgtgctcctgggtgcccgccctgcggcgctacaagatccaccctgacttctcgccatccgcgcagcagctgctaccttgcctggggcagaccctctaccagcatgtgatgtttgtgttccccgtgacgctgctgcattgggcccgcagcccggccctcctgccccacgaagctcccgagctgctcctgctgctgcaccacatcctgttctgcctgctactcttcgacatggagttcttcgtgtggcacctgctgcaccacaaggtgccctggctgtaccgcaccttccacaaggtgcaccaccagaactcgtcctcgttcgcgctggcaacgcagtatatgagcgtctgggaactgttttctttgggcttcttcgacatgatgaacgtcacactgctcgggtgccacccgctcaccaccctgaccttccacgtggtcaacatctggctttccgtggaggaccactccggctacaacttcccttggtccactcacagactggtgcccttcgggtggtacgggggtgtggtgcaccacgacctgcatcactctcactttaactgcaacttcgctccgtactttacacactgggacaaaatactgggaacgctgcggactgcatctgtcccagcgcgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05336_sequence/1
type: Sequence
persistent identity: BBa S05336 sequence
display id: BBa_S05336_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K844000_sequence/1
type: Sequence
persistent identity: BBa K844000 sequence
display id: BBa_K844000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcaccatcaccaccatcatcaccattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z