LOCUS       BBa_K1898250            1808 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong promoter + Strong RBS + GSR + 10x Histidine tag + Double 
            terminator
ACCESSION   BBa_K1898250
VERSION     BBa_K1898250.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..1627
                     /label=BBa_K1898200
     misc_feature    1636..1665
                     /label=10x-Histidine Tag
     misc_feature    1666..1668
                     /label=Stop
     misc_feature    1669..1671
                     /label=Stop
     misc_feature    1636..1671
                     /label=BBa_K844000
     stem_loop       1691..1734
                     /label=stem_loop
     misc_feature    1680..1759
                     /label=BBa_B0010
     terminator      1680..1759
                     /label=BBa_B0010
     stem_loop       1775..1794
                     /label=T7 TE
     misc_feature    1801..1801
                     /label=stop
     polyA_site      1795..1808
                     /label=polya
     misc_feature    1768..1808
                     /label=BBa_B0012
     terminator      1768..1808
                     /label=BBa_B0012
     terminator      1680..1808
                     /label=BBa_B0015
     misc_feature    1636..1808
                     /label=BBa_S05336
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccctg ctgccccgag ccctgagcgc cggcgcggga ccgagctggc ggcgggcggc
      121 gcgcgccttc cgaggcttcc tgctgcttct gcccgagccc gcggccctca cgcgcgccct
      181 ctcccgtgcc atggcctgta ggcaggagcc gcagccgcag ggcccgccgc ccgctgctgg
      241 cgccgtggcc tcctatgact acctggtgat cgggggcggc tcgggcgggc tggccagcgc
      301 gcgcagggcg gccgagctgg gtgccagggc cgccgtggtg gagagccaca agctgggtgg
      361 cacttgcgtg aatgttggat gtgtacccaa aaaggtaatg tggaacacag ctgtccactc
      421 tgagttcatg catgatcatg ctgattatgg ctttccaagt tgtgagggta aattcaattg
      481 gcgtgttatt aaggaaaagc gggatgccta tgtgagccgc ctgaatgcca tctatcaaaa
      541 caatctcacc aagtcccata tagaaatcat ccgtggccat gcagccttca cgagtgatcc
      601 caagcccaca atagaggtca gtgggaaaaa gtacaccgcc ccacacatcc tgatcgccac
      661 aggtggtatg ccctccaccc ctcatgagag ccagatcccc ggtgccagct taggaataac
      721 cagcgatgga ttttttcagc tggaagaatt gcccggccgc agcgtcattg ttggtgcagg
      781 ttacattgct gtggagatgg cagggatcct gtcagccctg ggttctaaga catcactgat
      841 gatacggcat gataaggtac ttagaagttt tgattcaatg atcagcacca actgcacgga
      901 ggagctggag aacgctggcg tggaggtgct gaagttctcc caggtcaagg aggttaaaaa
      961 gactttgtcg ggcttggaag tcagcatggt tactgctgtt cccggtaggc taccagtcat
     1021 gaccatgatt ccagatgttg actgcctgct ctgggccatt gggcgggtcc cgaataccaa
     1081 ggacctgagt ttaaacaaac tggggattca aaccgatgac aagggtcata tcatcgtaga
     1141 cgagttccag aataccaacg tcaaaggcat ctatgcagtt ggggatgtat gtggaaaagc
     1201 tcttcttact ccagttgcaa tagctgctgg ccgaaaactt gcccatcgac tttttgaata
     1261 taaggaagat tccaaattag attataacaa catcccaact gtggtcttca gccacccccc
     1321 tattgggaca gtgggactca cggaagatga agccattcat aaatatggaa tagaaaatgt
     1381 gaagacctat tcaacgagct ttaccccgat gtatcacgca gttaccaaaa ggaaaacaaa
     1441 atgtgtgatg aaaatggtct gtgctaacaa ggaagaaaag gtggttggga tccatatgca
     1501 gggacttggg tgtgatgaaa tgctacaggg ttttgctgtt gcagtgaaga tgggagcaac
     1561 gaaggcagac tttgacaaca cagtcgccat tcaccctacc tcttcagaag agctggtcac
     1621 acttcgttac tagagcatca tcaccatcac caccatcatc accattaata atactagagc
     1681 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1741 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1801 cgtttata
//