##gff-version 3
##sequence-region BBa_K1898250 1 1808
BBa_K1898250	.	engineered_region	1636	1808	.	+	0	ID=BBa_S05336;Name=BBa_S05336
BBa_K1898250	.	terminator	1680	1808	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05336
BBa_K1898250	.	terminator	1680	1759	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1898250	.	stem_loop	1691	1734	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1898250	.	engineered_region	1680	1759	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1898250	.	terminator	1768	1808	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1898250	.	stem_loop	1775	1794	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1898250	.	polyA_site	1795	1808	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1898250	.	stop_codon	1801	1801	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1898250	.	engineered_region	1768	1808	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1898250	.	tag	1636	1671	.	+	0	ID=BBa_K844000;Name=BBa_K844000;Parent=BBa_S05336
BBa_K1898250	.	sequence_feature	1636	1665	.	+	0	ID=annotation2206607;Name=10x-Histidine Tag;Parent=BBa_K844000
BBa_K1898250	.	stop_codon	1669	1671	.	+	0	ID=annotation2206609;Name=Stop;Parent=BBa_K844000
BBa_K1898250	.	stop_codon	1666	1668	.	+	0	ID=annotation2206608;Name=Stop;Parent=BBa_K844000
BBa_K1898250	.	engineered_region	1	55	.	+	0	ID=BBa_K880005;Name=BBa_K880005
BBa_K1898250	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K880005
BBa_K1898250	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1898250	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K880005
BBa_K1898250	.	CDS	62	1627	.	+	0	ID=BBa_K1898200;Name=BBa_K1898200
>BBa_K1898250
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccctgctgccccga
gccctgagcgccggcgcgggaccgagctggcggcgggcggcgcgcgccttccgaggcttcctgctgcttctgcccgagc
ccgcggccctcacgcgcgccctctcccgtgccatggcctgtaggcaggagccgcagccgcagggcccgccgcccgctgc
tggcgccgtggcctcctatgactacctggtgatcgggggcggctcgggcgggctggccagcgcgcgcagggcggccgag
ctgggtgccagggccgccgtggtggagagccacaagctgggtggcacttgcgtgaatgttggatgtgtacccaaaaagg
taatgtggaacacagctgtccactctgagttcatgcatgatcatgctgattatggctttccaagttgtgagggtaaatt
caattggcgtgttattaaggaaaagcgggatgcctatgtgagccgcctgaatgccatctatcaaaacaatctcaccaag
tcccatatagaaatcatccgtggccatgcagccttcacgagtgatcccaagcccacaatagaggtcagtgggaaaaagt
acaccgccccacacatcctgatcgccacaggtggtatgccctccacccctcatgagagccagatccccggtgccagctt
aggaataaccagcgatggattttttcagctggaagaattgcccggccgcagcgtcattgttggtgcaggttacattgct
gtggagatggcagggatcctgtcagccctgggttctaagacatcactgatgatacggcatgataaggtacttagaagtt
ttgattcaatgatcagcaccaactgcacggaggagctggagaacgctggcgtggaggtgctgaagttctcccaggtcaa
ggaggttaaaaagactttgtcgggcttggaagtcagcatggttactgctgttcccggtaggctaccagtcatgaccatg
attccagatgttgactgcctgctctgggccattgggcgggtcccgaataccaaggacctgagtttaaacaaactgggga
ttcaaaccgatgacaagggtcatatcatcgtagacgagttccagaataccaacgtcaaaggcatctatgcagttgggga
tgtatgtggaaaagctcttcttactccagttgcaatagctgctggccgaaaacttgcccatcgactttttgaatataag
gaagattccaaattagattataacaacatcccaactgtggtcttcagccacccccctattgggacagtgggactcacgg
aagatgaagccattcataaatatggaatagaaaatgtgaagacctattcaacgagctttaccccgatgtatcacgcagt
taccaaaaggaaaacaaaatgtgtgatgaaaatggtctgtgctaacaaggaagaaaaggtggttgggatccatatgcag
ggacttgggtgtgatgaaatgctacagggttttgctgttgcagtgaagatgggagcaacgaaggcagactttgacaaca
cagtcgccattcaccctacctcttcagaagagctggtcacacttcgttactagagcatcatcaccatcaccaccatcat
caccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata