LOCUS       BBa_K1898450             767 bp    DNA     linear       26-Nov-2024
DEFINITION  Strong promoter + Strong RBS + CRYAB + 10x Histidine tag + Double 
            terminator
ACCESSION   BBa_K1898450
VERSION     BBa_K1898450.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..586
                     /label=BBa_K1898400
     misc_feature    595..624
                     /label=10x-Histidine Tag
     misc_feature    625..627
                     /label=Stop
     misc_feature    628..630
                     /label=Stop
     misc_feature    595..630
                     /label=BBa_K844000
     stem_loop       650..693
                     /label=stem_loop
     misc_feature    639..718
                     /label=BBa_B0010
     terminator      639..718
                     /label=BBa_B0010
     stem_loop       734..753
                     /label=T7 TE
     misc_feature    760..760
                     /label=stop
     polyA_site      754..767
                     /label=polya
     misc_feature    727..767
                     /label=BBa_B0012
     terminator      727..767
                     /label=BBa_B0012
     terminator      639..767
                     /label=BBa_B0015
     misc_feature    595..767
                     /label=BBa_S05336
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggacatc gccatccacc acccctggat ccgccgcccc ttctttcctt tccactcccc
      121 cagccgcctc tttgaccagt tcttcggaga gcacctgttg gagtctgatc ttttcccgac
      181 gtctacttcc ctgagtccct tctaccttcg gccaccctcc ttcctgcggg cacccagctg
      241 gtttgacact ggactctcag agatgcgcct ggagaaggac aggttctctg tcaacctgga
      301 tgtgaagcac ttctccccag aggaactcaa agttaaggtg ttgggagatg tgattgaggt
      361 gcatggaaaa catgaagagc gccaggatga acatggtttc atctccaggg agttccacag
      421 gaaataccgg atcccagctg atgtagaccc tctcaccatt acttcatccc tgtcatctga
      481 tggggtcctc actgtgaatg gaccaaggaa acaggtctct ggccctgagc gcaccattcc
      541 catcacccgt gaagagaagc ctgctgtcac cgcagccccc aagaaatact agagcatcat
      601 caccatcacc accatcatca ccattaataa tactagagcc aggcatcaaa taaaacgaaa
      661 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      721 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//