LOCUS       BBa_K1898550             962 bp    DNA     linear       30-Dec-2025
DEFINITION  Strong promoter + Strong RBS + GFP + 10x Histidine tag + double 
            terminator
ACCESSION   BBa_K1898550
VERSION     BBa_K1898550.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     misc_feature    790..819
                     /label=10x-Histidine Tag
     misc_feature    820..822
                     /label=Stop
     misc_feature    823..825
                     /label=Stop
     misc_feature    790..825
                     /label=BBa_K844000
     stem_loop       845..888
                     /label=stem_loop
     misc_feature    834..913
                     /label=BBa_B0010
     terminator      834..913
                     /label=BBa_B0010
     stem_loop       929..948
                     /label=T7 TE
     misc_feature    955..955
                     /label=stop
     polyA_site      949..962
                     /label=polya
     misc_feature    922..962
                     /label=BBa_B0012
     terminator      922..962
                     /label=BBa_B0012
     terminator      834..962
                     /label=BBa_B0015
     misc_feature    790..962
                     /label=BBa_S05336
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc atcatcacca tcaccaccat catcaccatt aataatacta gagccaggca
      841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      961 ta
//