LOCUS BBa_K1899000 7891 bp DNA linear 25-Nov-2024
DEFINITION tetp-BFP-phlFp-RFP-lacp-GFP
ACCESSION BBa_K1899000
VERSION BBa_K1899000.1
FEATURES Location/Qualifiers
misc_feature 1..19
/label=TetR 1
misc_feature 1..54
/label=BBa_R0040
promoter 20..25
/label=-35
misc_feature 26..44
/label=TetR 2
promoter 43..48
/label=-10
promoter 1..54
/label=BBa_R0040
RBS 63..75
/label=RBS-3\Weak
RBS 69..72
/label=RBS
misc_feature 63..75
/label=BBa_B0032
RBS 63..75
/label=BBa_B0032
CDS 82..681
/label=phlF
CDS 82..684
/label=BBa_K1725040
misc_feature 63..684
/label=BBa_S05326
RBS 693..705
/label=RBS-3\Weak
RBS 699..702
/label=RBS
misc_feature 693..705
/label=BBa_B0032
RBS 693..705
/label=BBa_B0032
misc_feature 1801..1839
/label=LVA
CDS 712..1839
/label=lacI-LVA
misc_feature 712..1839
/label=BBa_C0012
misc_feature 1840..1864
/label=Help:Barcodes
CDS 712..1839
/label=BBa_C0012
misc_feature 693..1864
/label=BBa_K1899009
RBS 1873..1885
/label=RBS-3\Weak
RBS 1879..1882
/label=RBS
misc_feature 1873..1885
/label=BBa_B0032
RBS 1873..1885
/label=BBa_B0032
CDS 1892..2590
/label=mTagBFP
CDS 1892..2596
/label=BBa_K592100
misc_feature 1873..2596
/label=BBa_K592023
misc_feature 2605..2643
/label=B1006
stem_loop 2614..2635
/label=modified thr terminator
polyA_site 2636..2643
/label=PolyA
polyA_site 2605..2613
/label=PolyA
terminator 2605..2643
/label=BBa_B1006
misc_feature 1873..2643
/label=BBa_K1899001
promoter 2652..2717
/label=BBa_K1899004
RBS 2726..2738
/label=RBS-3\Weak
RBS 2732..2735
/label=RBS
misc_feature 2726..2738
/label=BBa_B0032
RBS 2726..2738
/label=BBa_B0032
misc_feature 3834..3872
/label=LVA
CDS 2745..3872
/label=lacI-LVA
misc_feature 2745..3872
/label=BBa_C0012
misc_feature 3873..3897
/label=Help:Barcodes
CDS 2745..3872
/label=BBa_C0012
misc_feature 2726..3897
/label=BBa_K1899009
RBS 3906..3918
/label=RBS-3\Weak
RBS 3912..3915
/label=RBS
misc_feature 3906..3918
/label=BBa_B0032
RBS 3906..3918
/label=BBa_B0032
CDS 3928..4544
/label=tetR
misc_feature 4545..4578
/label=SsrA
misc_feature 4585..4609
/label=Help:Barcodes
CDS 3925..4609
/label=BBa_C0040
misc_feature 3906..4609
/label=BBa_K1899010
RBS 4618..4630
/label=RBS-3\Weak
RBS 4624..4627
/label=RBS
misc_feature 4618..4630
/label=BBa_B0032
RBS 4618..4630
/label=BBa_B0032
CDS 4637..5311
/label=mrfp1
misc_feature 5318..5342
/label=Help:Barcodes
CDS 4637..5342
/label=BBa_E1010
stem_loop 5362..5405
/label=stem_loop
misc_feature 5351..5430
/label=BBa_B0010
terminator 5351..5430
/label=BBa_B0010
stem_loop 5446..5465
/label=T7 TE
misc_feature 5472..5472
/label=stop
polyA_site 5466..5479
/label=polya
misc_feature 5439..5479
/label=BBa_B0012
terminator 5439..5479
/label=BBa_B0012
terminator 5351..5479
/label=BBa_B0015
misc_feature 4618..5479
/label=BBa_K516032
CDS 5488..5575
/label=end of LacI coding region (inactive)
misc_feature 5488..5687
/label=BBa_R0010
misc_feature 5576..5613
/label=CAP binding site
promoter 5624..5629
/label=-35
promoter 5648..5653
/label=-10
misc_feature 5653..5687
/label=LacI binding site
misc_feature 5660..5660
/label=start
promoter 5488..5687
/label=BBa_R0010
RBS 5696..5708
/label=RBS-3\Weak
RBS 5702..5705
/label=RBS
misc_feature 5696..5708
/label=BBa_B0032
RBS 5696..5708
/label=BBa_B0032
CDS 5718..6334
/label=tetR
misc_feature 6335..6368
/label=SsrA
misc_feature 6375..6399
/label=Help:Barcodes
CDS 5715..6399
/label=BBa_C0040
misc_feature 5696..6399
/label=BBa_K1899010
RBS 6408..6420
/label=RBS-3\Weak
RBS 6414..6417
/label=RBS
misc_feature 6408..6420
/label=BBa_B0032
RBS 6408..6420
/label=BBa_B0032
CDS 6427..7026
/label=phlF
CDS 6427..7029
/label=BBa_K1725040
misc_feature 6408..7029
/label=BBa_S05326
RBS 7038..7049
/label=BBa_B0032
CDS 7057..7770
/label=sfGFP
misc_feature 7050..7056
/label=scar
misc_feature 7779..7891
/label=BBa_J61048
misc_feature 7771..7778
/label=scar
misc_feature 7038..7891
/label=BBa_K1899003
ORIGIN
1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
61 agtcacacag gaaagtacta gatggcacgt accccgagcc gtagcagcat tggtagcctg
121 cgtagtccgc atacccataa agcaattctg accagcacca ttgaaatcct gaaagaatgt
181 ggttatagcg gtctgagcat tgaaagcgtt gcacgtcgtg ccggtgcaag caaaccgacc
241 atttatcgtt ggtggaccaa taaagcagca ctgattgccg aagtgtatga aaatgaaagc
301 gaacaggtgc gtaaatttcc ggatctgggt agctttaaag ccgatctgga ttttctgctg
361 cgtaatctgt ggaaagtttg gcgtgaaacc atttgtggtg aagcatttcg ttgtgttatt
421 gcagaagcac agctggaccc tgcaaccctg acccagctga aagatcagtt tatggaacgt
481 cgtcgtgaga tgccgaaaaa actggttgaa aatgccatta gcaatggtga actgccgaaa
541 gataccaatc gtgaactgct gctggatatg atttttggtt tttgttggta tcgcctgctg
601 accgaacagc tgaccgttga acaggatatt gaagaattta ccttcctgct gattaatggt
661 gtttgtccgg gtacacagcg ttaatactag agtcacacag gaaagtacta gatggtgaat
721 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
781 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
841 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
901 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
961 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
1021 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
1081 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
1141 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
1201 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
1261 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
1321 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
1381 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
1441 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
1501 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
1561 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
1621 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
1681 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
1741 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
1801 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
1861 tctctactag agtcacacag gaaagtacta gatgagcgaa ctgatcaaag agaacatgca
1921 catgaagctg tacatggaag gcaccgttga caaccaccac tttaagtgca cgtctgaggg
1981 tgagggtaag ccgtacgaag gcacccaaac catgcgtatc aaagttgtgg agggcggtcc
2041 actgccgttc gcttttgaca ttctggcgac cagcttcctg tacggttcca aaacgttcat
2101 taaccatact cagggcattc cggatttctt caaacagagc tttccggaag gtttcacctg
2161 ggagcgtgtc accacgtatg aagatggtgg tgtgttgacc gccacccaag atacctccct
2221 gcaagatggc tgtctgatct ataacgtgaa aattcgtggc gtcaacttta cgagcaatgg
2281 tccggtgatg cagaagaaaa ccctgggttg ggaggcgttt acggaaaccc tgtatccggc
2341 cgatggtggc ctggagggcc gtaacgacat ggcactgaag ctggttggtg gcagccattt
2401 gatcgcaaat atcaagacga cgtaccgcag caagaaaccg gcgaaaaatc tgaagatgcc
2461 gggtgtttac tatgtcgact accgtctgga acgcattaaa gaagcgaata atgagactta
2521 cgtggagcag cacgaggttg cagtcgcgcg ctattgcgac ttgcctagca agctgggtca
2581 taaactgaat taataatact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt
2641 ttttactaga gcgacgtacg gtggaatctg attcgttacc aattgacatg atacgaaacg
2701 taccgtatcg ttaaggttac tagagtcaca caggaaagta ctagatggtg aatgtgaaac
2761 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
2821 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
2881 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
2941 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
3001 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
3061 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
3121 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
3181 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
3241 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
3301 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
3361 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
3421 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
3481 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
3541 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
3601 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
3661 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
3721 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
3781 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa
3841 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac
3901 tagagtcaca caggaaagta ctagatgtcc agattagata aaagtaaagt gattaacagc
3961 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
4021 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
4081 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
4141 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
4201 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
4261 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
4321 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
4381 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
4441 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
4501 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa
4561 aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact actagagtca
4621 cacaggaaag tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
4681 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
4741 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
4801 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
4861 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
4921 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
4981 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
5041 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
5101 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
5161 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
5221 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
5281 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
5341 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
5401 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
5461 gggcctttct gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc
5521 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
5581 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
5641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagtcaca
5701 caggaaagta ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
5761 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
5821 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
5881 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
5941 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
6001 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
6061 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
6121 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
6181 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
6241 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
6301 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
6361 tagtagctta ataacactga tagtgctagt gtagatcact actagagtca cacaggaaag
6421 tactagatgg cacgtacccc gagccgtagc agcattggta gcctgcgtag tccgcatacc
6481 cataaagcaa ttctgaccag caccattgaa atcctgaaag aatgtggtta tagcggtctg
6541 agcattgaaa gcgttgcacg tcgtgccggt gcaagcaaac cgaccattta tcgttggtgg
6601 accaataaag cagcactgat tgccgaagtg tatgaaaatg aaagcgaaca ggtgcgtaaa
6661 tttccggatc tgggtagctt taaagccgat ctggattttc tgctgcgtaa tctgtggaaa
6721 gtttggcgtg aaaccatttg tggtgaagca tttcgttgtg ttattgcaga agcacagctg
6781 gaccctgcaa ccctgaccca gctgaaagat cagtttatgg aacgtcgtcg tgagatgccg
6841 aaaaaactgg ttgaaaatgc cattagcaat ggtgaactgc cgaaagatac caatcgtgaa
6901 ctgctgctgg atatgatttt tggtttttgt tggtatcgcc tgctgaccga acagctgacc
6961 gttgaacagg atattgaaga atttaccttc ctgctgatta atggtgtttg tccgggtaca
7021 cagcgttaat actagagtca cacaggaaag tactagatga gcaaaggaga agaacttttc
7081 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
7141 gtccgtggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
7201 actactggaa aactacctgt tccatggcca acacttgtca ctactctgac ctatggtgtt
7261 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
7321 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
7381 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt
7441 gattttaaag aagatggaaa cattctcgga cacaaactcg agtacaactt taactcacac
7501 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
7561 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
7621 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
7681 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
7741 attacacatg gcatggatga gctctacaaa tactagagcc ggcttatcgg tcagtttcac
7801 ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa
7861 tgactgtcca cgacgctata cccaaaagaa a
//