BBa_K1899000
type: ComponentDefinition
persistent identity: BBa K1899000
display id: BBa_K1899000
version: 1
was generated by: iGEM to SBOL conversion
description: tetp-BFP-phlFp-RFP-lacp-GFP
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T05:38:39Z
mutable provenance: Most of the parts come from the Part Registry, except for the BBa_K1899004, sequence of which is obtained from the paper, Genomic mining of prokaryotic repressors for orthogonal logic gates.
owned by: myers
owned by: james
mutable description: It is the main construct which is expected to perform as a tristable switch with three discrete, but alternating, steady states. By applying different inducers, three repressible promoters would act accordingly, producing three different outputs.
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 0
m user id: 26182
owner id: 26182
owning group id: 9
sample status: Not in stock
status: status/Unavailable
status: status/Planning
bookmark: false
mutable notes: NIL
star: false
creator: iGEM16_HKUST
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0010
component: BBa_K516032
component: BBa_K1899010
component: BBa_K1899009
component: BBa_K1899003
component: BBa_S05326
component: BBa_R0040
component: BBa_K1899004
component: BBa_K1899001
sequence annotation: BBa_R0040
sequence annotation: BBa_S05326
sequence annotation: BBa_K1899009
sequence annotation: BBa_K1899001
sequence annotation: BBa_K1899004
sequence annotation: BBa_K1899010
sequence annotation: BBa_K516032
sequence annotation: BBa_R0010
sequence annotation: BBa_K1899003
sequence: https://synbiohub.org/public/igem/BBa_K1899000_sequence/1

BBa_R0010
type: Component
persistent identity: component2519152
display id: component2519152
version: 1
top level: BBa_K1899000
definition: LacI
access: public

BBa_K516032
type: Component
persistent identity: component2519151
display id: component2519151
version: 1
top level: BBa_K1899000
definition: BBa_K516032
access: public

BBa_K1899010
type: Component
persistent identity: component2519137
display id: component2519137
version: 1
top level: BBa_K1899000
definition: BBa_K1899010
access: public

BBa_K1899009
type: Component
persistent identity: component2519129
display id: component2519129
version: 1
top level: BBa_K1899000
definition: BBa_K1899009
access: public

BBa_K1899003
type: Component
persistent identity: component2519178
display id: component2519178
version: 1
top level: BBa_K1899000
definition: BBa_K1899003
access: public

BBa_S05326
type: Component
persistent identity: component2519172
display id: component2519172
version: 1
top level: BBa_K1899000
definition: BBa_S05326
access: public

BBa_K1899010
type: Component
persistent identity: component2519166
display id: component2519166
version: 1
top level: BBa_K1899000
definition: BBa_K1899010
access: public

BBa_R0040
type: Component
persistent identity: component2519090
display id: component2519090
version: 1
top level: BBa_K1899000
definition: p(tetR)
access: public

BBa_K1899004
type: Component
persistent identity: component2519121
display id: component2519121
version: 1
top level: BBa_K1899000
definition: BBa_K1899004
access: public

BBa_K1899001
type: Component
persistent identity: component2519120
display id: component2519120
version: 1
top level: BBa_K1899000
definition: BBa_K1899001
access: public

BBa_K1899009
type: Component
persistent identity: component2519108
display id: component2519108
version: 1
top level: BBa_K1899000
definition: BBa_K1899009
access: public

BBa_S05326
type: Component
persistent identity: component2519100
display id: component2519100
version: 1
top level: BBa_K1899000
definition: BBa_S05326
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2519090
display id: annotation2519090
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519090/range2519090/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519090/range2519090/1
type: Range
persistent identity: range2519090
display id: range2519090
version: 1
top level: BBa_K1899000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_S05326
type: SequenceAnnotation
persistent identity: annotation2519100
display id: annotation2519100
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519100/range2519100/1
component: BBa_S05326

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type: Range
persistent identity: range2519100
display id: range2519100
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 63
end: 684
orientation: inline

BBa_K1899009
type: SequenceAnnotation
persistent identity: annotation2519108
display id: annotation2519108
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519108/range2519108/1
component: BBa_K1899009

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519108/range2519108/1
type: Range
persistent identity: range2519108
display id: range2519108
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 693
end: 1864
orientation: inline

BBa_K1899001
type: SequenceAnnotation
persistent identity: annotation2519120
display id: annotation2519120
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519120/range2519120/1
component: BBa_K1899001

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519120/range2519120/1
type: Range
persistent identity: range2519120
display id: range2519120
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 1873
end: 2643
orientation: inline

BBa_K1899004
type: SequenceAnnotation
persistent identity: annotation2519121
display id: annotation2519121
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519121/range2519121/1
component: BBa_K1899004

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519121/range2519121/1
type: Range
persistent identity: range2519121
display id: range2519121
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 2652
end: 2717
orientation: inline

BBa_K1899009
type: SequenceAnnotation
persistent identity: annotation2519129
display id: annotation2519129
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519129/range2519129/1
component: BBa_K1899009

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519129/range2519129/1
type: Range
persistent identity: range2519129
display id: range2519129
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 2726
end: 3897
orientation: inline

BBa_K1899010
type: SequenceAnnotation
persistent identity: annotation2519137
display id: annotation2519137
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519137/range2519137/1
component: BBa_K1899010

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519137/range2519137/1
type: Range
persistent identity: range2519137
display id: range2519137
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 3906
end: 4609
orientation: inline

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2519151
display id: annotation2519151
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519151/range2519151/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519151/range2519151/1
type: Range
persistent identity: range2519151
display id: range2519151
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 4618
end: 5479
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2519152
display id: annotation2519152
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519152/range2519152/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519152/range2519152/1
type: Range
persistent identity: range2519152
display id: range2519152
version: 1
top level: BBa_K1899000
direction: direction/forward
start: 5488
end: 5687
orientation: inline

BBa_K1899010
type: SequenceAnnotation
persistent identity: annotation2519166
display id: annotation2519166
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519166/range2519166/1
component: BBa_K1899010

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519166/range2519166/1
type: Range
persistent identity: range2519166
display id: range2519166
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 5696
end: 6399
orientation: inline

BBa_S05326
type: SequenceAnnotation
persistent identity: annotation2519172
display id: annotation2519172
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519172/range2519172/1
component: BBa_S05326

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519172/range2519172/1
type: Range
persistent identity: range2519172
display id: range2519172
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 6408
end: 7029
orientation: inline

BBa_K1899003
type: SequenceAnnotation
persistent identity: annotation2519178
display id: annotation2519178
version: 1
top level: BBa_K1899000
location: https://synbiohub.org/public/igem/BBa_K1899000/annotation2519178/range2519178/1
component: BBa_K1899003

https://synbiohub.org/public/igem/BBa_K1899000/annotation2519178/range2519178/1
type: Range
persistent identity: range2519178
display id: range2519178
version: 1
top level: BBa_K1899000
direction: direction/reverse
start: 7038
end: 7891
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1899001
type: ComponentDefinition
persistent identity: BBa K1899001
display id: BBa_K1899001
version: 1
was generated by: iGEM to SBOL conversion
description: Blue Fluorescent Protein mTagBFP generator (Medium RBS)
created: 2016-09-19T11:00:00Z
modified: 2016-09-21T09:47:51Z
mutable provenance: NIL
owned by: james
owned by: myers
mutable description: Improved version of BBa_K592023 by adding a terminator BBa_B1006 3???to the part.
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NIL
star: false
creator: iGEM2016_HKUST
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B1006
component: BBa_K592023
sequence annotation: BBa_K592023
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1899001_sequence/1

BBa_B1006
type: Component
persistent identity: component2484336
display id: component2484336
version: 1
top level: BBa_K1899001
definition: BBa_B1006
access: public

BBa_K592023
type: Component
persistent identity: component2484331
display id: component2484331
version: 1
top level: BBa_K1899001
definition: BBa_K592023
access: public

BBa_K592023
type: SequenceAnnotation
persistent identity: annotation2484331
display id: annotation2484331
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
component: BBa_K592023

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
type: Range
persistent identity: range2484331
display id: range2484331
version: 1
top level: BBa_K1899001
direction: direction/reverse
start: 1
end: 724
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2484336
display id: annotation2484336
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
type: Range
persistent identity: range2484336
display id: range2484336
version: 1
top level: BBa_K1899001
direction: direction/reverse
start: 733
end: 771
orientation: inline

BBa_S05326
type: ComponentDefinition
persistent identity: BBa S05326
display id: BBa_S05326
version: 1
was generated by: iGEM to SBOL conversion
description: B0032-phlF
created: 2016-09-23T11:00:00Z
modified: 2016-09-24T09:00:12Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 29868
owner id: 29868
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: HKUST_iGEM 2016
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1725040
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K1725040
sequence: https://synbiohub.org/public/igem/BBa_S05326_sequence/1

BBa_K1725040
type: Component
persistent identity: component2484856
display id: component2484856
version: 1
top level: BBa_S05326
definition: phlF
access: public

BBa_B0032
type: Component
persistent identity: component2484853
display id: component2484853
version: 1
top level: BBa_S05326
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2484853
display id: annotation2484853
version: 1
top level: BBa_S05326
location: https://synbiohub.org/public/igem/BBa_S05326/annotation2484853/range2484853/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S05326/annotation2484853/range2484853/1
type: Range
persistent identity: range2484853
display id: range2484853
version: 1
top level: BBa_S05326
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K1725040
type: SequenceAnnotation
persistent identity: annotation2484856
display id: annotation2484856
version: 1
top level: BBa_S05326
location: https://synbiohub.org/public/igem/BBa_S05326/annotation2484856/range2484856/1
component: BBa_K1725040

https://synbiohub.org/public/igem/BBa_S05326/annotation2484856/range2484856/1
type: Range
persistent identity: range2484856
display id: range2484856
version: 1
top level: BBa_S05326
direction: direction/reverse
start: 20
end: 622
orientation: inline

BBa_K1899004
type: ComponentDefinition
persistent identity: BBa K1899004
display id: BBa_K1899004
version: 1
was generated by: iGEM to SBOL conversion
description: phlFp promoter
created: 2016-10-01T11:00:00Z
modified: 2016-10-18T08:17:48Z
mutable provenance: http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: PphlF is a constitutive promoter. repressed by phlF repressor(K1725040) induced 2,4-Diacetylphloroglucinol ( DAPG ) inducer.
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26182
owner id: 29868
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Since it is a new promoter, forward and reverse oligos of PphlF with XbaI and PstI sticky ends were designed and ordered, which were subsequently phosphorylated and annealed.
star: false
creator: iGEM2016_HKUST
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1899004_sequence/1

BBa_K1899009
type: ComponentDefinition
persistent identity: BBa K1899009
display id: BBa_K1899009
version: 1
was generated by: iGEM to SBOL conversion
description: B0032-LacI
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T10:26:12Z
mutable provenance: NIL
owned by: james
owned by: myers
mutable description: LacI is conjugated to a medium strength RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NIL
star: false
creator: iGEM2016_HKUST
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0012
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K1899009_sequence/1

BBa_C0012
type: Component
persistent identity: component2517238
display id: component2517238
version: 1
top level: BBa_K1899009
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component2517235
display id: component2517235
version: 1
top level: BBa_K1899009
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2517235
display id: annotation2517235
version: 1
top level: BBa_K1899009
location: https://synbiohub.org/public/igem/BBa_K1899009/annotation2517235/range2517235/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1899009/annotation2517235/range2517235/1
type: Range
persistent identity: range2517235
display id: range2517235
version: 1
top level: BBa_K1899009
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2517238
display id: annotation2517238
version: 1
top level: BBa_K1899009
location: https://synbiohub.org/public/igem/BBa_K1899009/annotation2517238/range2517238/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K1899009/annotation2517238/range2517238/1
type: Range
persistent identity: range2517238
display id: range2517238
version: 1
top level: BBa_K1899009
direction: direction/forward
start: 20
end: 1147
orientation: inline

BBa_K1899010
type: ComponentDefinition
persistent identity: BBa K1899010
display id: BBa_K1899010
version: 1
was generated by: iGEM to SBOL conversion
description: B0032-TetR
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T10:30:41Z
mutable provenance: NIL
owned by: james
owned by: myers
mutable description: TetR is conjugated to a medium strength RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NIL
star: false
creator: iGEM2016_HKUST
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0040
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K1899010_sequence/1

BBa_C0040
type: Component
persistent identity: component2517259
display id: component2517259
version: 1
top level: BBa_K1899010
definition: tetR
access: public

BBa_B0032
type: Component
persistent identity: component2517254
display id: component2517254
version: 1
top level: BBa_K1899010
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2517254
display id: annotation2517254
version: 1
top level: BBa_K1899010
location: https://synbiohub.org/public/igem/BBa_K1899010/annotation2517254/range2517254/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1899010/annotation2517254/range2517254/1
type: Range
persistent identity: range2517254
display id: range2517254
version: 1
top level: BBa_K1899010
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2517259
display id: annotation2517259
version: 1
top level: BBa_K1899010
location: https://synbiohub.org/public/igem/BBa_K1899010/annotation2517259/range2517259/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K1899010/annotation2517259/range2517259/1
type: Range
persistent identity: range2517259
display id: range2517259
version: 1
top level: BBa_K1899010
direction: direction/reverse
start: 20
end: 704
orientation: inline

BBa_K516032
type: ComponentDefinition
persistent identity: BBa K516032
display id: BBa_K516032
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP protein generator TERM+ RBS+(B0032)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP protein generator TERM+ RBS+(B0032)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_E1010
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516032_sequence/1

BBa_B0015
type: Component
persistent identity: component2250608
display id: component2250608
version: 1
top level: BBa_K516032
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2250601
display id: component2250601
version: 1
top level: BBa_K516032
definition: mRFP1
access: public

BBa_B0032
type: Component
persistent identity: component2250597
display id: component2250597
version: 1
top level: BBa_K516032
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250597
display id: annotation2250597
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516032/annotation2250597/range2250597/1
type: Range
persistent identity: range2250597
display id: range2250597
version: 1
top level: BBa_K516032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250601
display id: annotation2250601
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516032/annotation2250601/range2250601/1
type: Range
persistent identity: range2250601
display id: range2250601
version: 1
top level: BBa_K516032
direction: direction/reverse
start: 20
end: 725
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250608
display id: annotation2250608
version: 1
top level: BBa_K516032
location: https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516032/annotation2250608/range2250608/1
type: Range
persistent identity: range2250608
display id: range2250608
version: 1
top level: BBa_K516032
direction: direction/reverse
start: 734
end: 862
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K1899003
type: ComponentDefinition
persistent identity: BBa K1899003
display id: BBa_K1899003
version: 1
was generated by: iGEM to SBOL conversion
description: Superfolder GFP
created: 2016-08-27T11:00:00Z
modified: 2016-10-15T04:03:09Z
mutable provenance: Waldo lab
owned by: james
owned by: myers
mutable description: Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: nil
star: false
creator: iGEM2016_HKUST
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_B0032
sequence annotation: scar
sequence annotation: sfGFP
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K1899003_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481802
display id: annotation2481802
version: 1
top level: BBa_K1899003
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
type: Range
persistent identity: range2481802
display id: range2481802
version: 1
top level: BBa_K1899003
direction: direction/forward
start: 1
end: 12
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481804
display id: annotation2481804
version: 1
top level: BBa_K1899003
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
type: Range
persistent identity: range2481804
display id: range2481804
version: 1
top level: BBa_K1899003
direction: direction/forward
start: 13
end: 19
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2481803
display id: annotation2481803
version: 1
top level: BBa_K1899003
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
type: Range
persistent identity: range2481803
display id: range2481803
version: 1
top level: BBa_K1899003
direction: direction/forward
start: 20
end: 733
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481806
display id: annotation2481806
version: 1
top level: BBa_K1899003
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
type: Range
persistent identity: range2481806
display id: range2481806
version: 1
top level: BBa_K1899003
direction: direction/forward
start: 734
end: 741
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2481805
display id: annotation2481805
version: 1
top level: BBa_K1899003
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
type: Range
persistent identity: range2481805
display id: range2481805
version: 1
top level: BBa_K1899003
direction: direction/forward
start: 742
end: 854
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

phlF
type: ComponentDefinition
persistent identity: BBa K1725040
display id: BBa_K1725040
version: 1
was generated by: iGEM to SBOL conversion
description: phlF encoding PhlF repressor from Pseudomonas protegens Pf-5
created: 2015-08-10T11:00:00Z
modified: 2016-07-23T11:16:49Z
mutable provenance: Sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
owned by: james
owned by: myers
mutable description: PhlF repressor from Pseudomonas protegens Pf-5. Represses promoter K1725000.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2146_
m user id: 27285
owner id: 27284
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Mhairi Davidson
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: phlF
sequence: https://synbiohub.org/public/igem/BBa_K1725040_sequence/1

phlF
type: SequenceAnnotation
persistent identity: annotation2473659
display id: annotation2473659
version: 1
top level: phlF
location: https://synbiohub.org/public/igem/BBa_K1725040/annotation2473659/range2473659/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1725040/annotation2473659/range2473659/1
type: Range
persistent identity: range2473659
display id: range2473659
version: 1
top level: phlF
direction: direction/forward
start: 1
end: 600
orientation: inline

BBa_K592023
type: ComponentDefinition
persistent identity: BBa K592023
display id: BBa_K592023
version: 1
was generated by: iGEM to SBOL conversion
description: B0032-BFP
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The BFP part is <partinfo>BBa_K592100</partinfo>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Blue Fluorescent Protein with B0032 RBS, this part can be added downstream to any promoter and then tested in FACS.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The BFP part (<partinfo>BBa_K592100</partinfo>) was codon-optimized for expression in E.coli by DNA2.0
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_K592100
sequence annotation: BBa_B0032
sequence annotation: BBa_K592100
sequence: https://synbiohub.org/public/igem/BBa_K592023_sequence/1

BBa_B0032
type: Component
persistent identity: component2136639
display id: component2136639
version: 1
top level: BBa_K592023
definition: BBa_B0032
access: public

BBa_K592100
type: Component
persistent identity: component2136642
display id: component2136642
version: 1
top level: BBa_K592023
definition: BFP
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2136639
display id: annotation2136639
version: 1
top level: BBa_K592023
location: https://synbiohub.org/public/igem/BBa_K592023/annotation2136639/range2136639/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K592023/annotation2136639/range2136639/1
type: Range
persistent identity: range2136639
display id: range2136639
version: 1
top level: BBa_K592023
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2136642
display id: annotation2136642
version: 1
top level: BBa_K592023
location: https://synbiohub.org/public/igem/BBa_K592023/annotation2136642/range2136642/1
component: BBa_K592100

https://synbiohub.org/public/igem/BBa_K592023/annotation2136642/range2136642/1
type: Range
persistent identity: range2136642
display id: range2136642
version: 1
top level: BBa_K592023
direction: direction/reverse
start: 20
end: 724
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BFP
type: ComponentDefinition
persistent identity: BBa K592100
display id: BBa_K592100
version: 1
was generated by: iGEM to SBOL conversion
description: Blue Fluorescent Protein (mTagBFP)
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T04:45:27Z
mutable provenance: Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
star: false
creator: Erik Gullberg
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mTagBFP
sequence: https://synbiohub.org/public/igem/BBa_K592100_sequence/1

mTagBFP
type: SequenceAnnotation
persistent identity: annotation2135490
display id: annotation2135490
version: 1
top level: BFP
location: https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1
type: Range
persistent identity: range2135490
display id: range2135490
version: 1
top level: BFP
direction: direction/forward
start: 1
end: 699
orientation: inline

https://synbiohub.org/public/igem/BBa_K1899000_sequence/1
type: Sequence
persistent identity: BBa K1899000 sequence
display id: BBa_K1899000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaatactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1899001_sequence/1
type: Sequence
persistent identity: BBa K1899001 sequence
display id: BBa_K1899001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05326_sequence/1
type: Sequence
persistent identity: BBa S05326 sequence
display id: BBa_S05326_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1899004_sequence/1
type: Sequence
persistent identity: BBa K1899004 sequence
display id: BBa_K1899004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgacgtacggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1899009_sequence/1
type: Sequence
persistent identity: BBa K1899009 sequence
display id: BBa_K1899009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1899010_sequence/1
type: Sequence
persistent identity: BBa K1899010 sequence
display id: BBa_K1899010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516032_sequence/1
type: Sequence
persistent identity: BBa K516032 sequence
display id: BBa_K516032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1899003_sequence/1
type: Sequence
persistent identity: BBa K1899003 sequence
display id: BBa_K1899003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1725040_sequence/1
type: Sequence
persistent identity: BBa K1725040 sequence
display id: BBa_K1725040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacgtaccccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctgaaagaatgtggttatagcggtctgagcattgaaagcgttgcacgtcgtgccggtgcaagcaaaccgaccatttatcgttggtggaccaataaagcagcactgattgccgaagtgtatgaaaatgaaagcgaacaggtgcgtaaatttccggatctgggtagctttaaagccgatctggattttctgctgcgtaatctgtggaaagtttggcgtgaaaccatttgtggtgaagcatttcgttgtgttattgcagaagcacagctggaccctgcaaccctgacccagctgaaagatcagtttatggaacgtcgtcgtgagatgccgaaaaaactggttgaaaatgccattagcaatggtgaactgccgaaagataccaatcgtgaactgctgctggatatgatttttggtttttgttggtatcgcctgctgaccgaacagctgaccgttgaacaggatattgaagaatttaccttcctgctgattaatggtgtttgtccgggtacacagcgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592023_sequence/1
type: Sequence
persistent identity: BBa K592023 sequence
display id: BBa_K592023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592100_sequence/1
type: Sequence
persistent identity: BBa K592100 sequence
display id: BBa_K592100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z