##gff-version 3
##sequence-region BBa_K1899006 1 2220
BBa_K1899006	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1899006	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1899006	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1899006	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1899006	.	promoter	1271	1336	.	+	0	ID=BBa_K1899004;Name=BBa_K1899004
BBa_K1899006	.	terminator	1224	1262	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K1899006	.	polyA_site	1255	1262	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1899006	.	stem_loop	1233	1254	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1899006	.	engineered_region	1224	1262	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1899006	.	polyA_site	1224	1232	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1899006	.	CDS	63	1190	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1899006	.	engineered_region	63	1190	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1899006	.	engineered_tag	1191	1215	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1899006	.	tag	1152	1190	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1899006	.	CDS	63	1190	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1899006	.	engineered_region	1345	2220	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1899006	.	terminator	2092	2171	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1899006	.	stem_loop	2103	2146	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1899006	.	engineered_region	2092	2171	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1899006	.	ribosome_entry_site	1345	1357	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1899006	.	engineered_region	1345	1357	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1899006	.	ribosome_entry_site	1345	1357	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1899006	.	ribosome_entry_site	1351	1354	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1899006	.	CDS	1364	2083	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1899006	.	CDS	1364	2083	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1899006	.	terminator	2180	2220	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1899006	.	polyA_site	2207	2220	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1899006	.	engineered_region	2180	2220	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1899006	.	stem_loop	2187	2206	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1899006	.	stop_codon	2213	2213	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1899006	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
>BBa_K1899006
tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggtgaatgtgaaacc
agtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaac
tggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggc
gattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaa
gcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattg
ctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatttt
ctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggc
ccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatag
cggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccac
tgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcg
gatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatt
ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgtt
gcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggttttttttta
ctagagcgacgtacggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactaga
gtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatgg
tgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcga
gatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatt
tttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtta
aaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtataca
tcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaact
agcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaa
tctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacac
atggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata