LOCUS       BBa_K1899007            2842 bp    DNA     linear       30-Jun-2024
DEFINITION  <i>phlFp</i>-B0032-Lacl-B0032-TetR-GFP
ACCESSION   BBa_K1899007
VERSION     BBa_K1899007.1  
FEATURES             Location/Qualifiers
     promoter        1..66
                     /label=BBa_K1899004
     RBS             75..87
                     /label=RBS-3\Weak
     RBS             81..84
                     /label=RBS
     misc_feature    75..87
                     /label=BBa_B0032
     RBS             75..87
                     /label=BBa_B0032
     misc_feature    1183..1221
                     /label=LVA
     CDS             94..1221
                     /label=lacI-LVA
     misc_feature    94..1221
                     /label=BBa_C0012
     misc_feature    1222..1246
                     /label=Help:Barcodes
     CDS             94..1221
                     /label=BBa_C0012
     RBS             1255..1267
                     /label=RBS-3\Weak
     RBS             1261..1264
                     /label=RBS
     misc_feature    1255..1267
                     /label=BBa_B0032
     RBS             1255..1267
                     /label=BBa_B0032
     CDS             1277..1893
                     /label=tetR
     misc_feature    1894..1927
                     /label=SsrA
     misc_feature    1934..1958
                     /label=Help:Barcodes
     CDS             1274..1958
                     /label=BBa_C0040
     RBS             1967..1979
                     /label=RBS-3\Weak
     RBS             1973..1976
                     /label=RBS
     misc_feature    1967..1979
                     /label=BBa_B0032
     RBS             1967..1979
                     /label=BBa_B0032
     CDS             1986..2705
                     /label=GFP protein
     CDS             1986..2705
                     /label=BBa_E0040
     stem_loop       2725..2768
                     /label=stem_loop
     misc_feature    2714..2793
                     /label=BBa_B0010
     terminator      2714..2793
                     /label=BBa_B0010
     stem_loop       2809..2828
                     /label=T7 TE
     misc_feature    2835..2835
                     /label=stop
     polyA_site      2829..2842
                     /label=polya
     misc_feature    2802..2842
                     /label=BBa_B0012
     terminator      2802..2842
                     /label=BBa_B0012
     misc_feature    1967..2842
                     /label=BBa_E0240
ORIGIN
        1 cgacgtacgg tggaatctga ttcgttacca attgacatga tacgaaacgt accgtatcgt
       61 taaggttact agagtcacac aggaaagtac tagatggtga atgtgaaacc agtaacgtta
      121 tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
      181 gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
      241 tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt
      301 gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
      361 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
      421 tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
      481 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
      541 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
      601 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
      661 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
      721 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
      781 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
      841 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
      901 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta
      961 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     1021 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     1081 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1141 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac
     1201 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagtcacac
     1261 aggaaagtac tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct
     1321 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     1381 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     1441 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     1501 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     1561 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     1621 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     1681 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     1741 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     1801 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     1861 attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt
     1921 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt
     1981 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     2041 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     2101 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
     2161 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
     2221 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     2281 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     2341 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     2401 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     2461 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
     2521 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     2581 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     2641 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     2701 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2761 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2821 ggtgggcctt tctgcgttta ta
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