##gff-version 3
##sequence-region BBa_K1899007 1 2842
BBa_K1899007	.	engineered_region	1967	2842	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1899007	.	terminator	2802	2842	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1899007	.	stem_loop	2809	2828	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1899007	.	stop_codon	2835	2835	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1899007	.	polyA_site	2829	2842	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1899007	.	engineered_region	2802	2842	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1899007	.	CDS	1986	2705	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1899007	.	CDS	1986	2705	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1899007	.	terminator	2714	2793	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1899007	.	stem_loop	2725	2768	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1899007	.	engineered_region	2714	2793	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1899007	.	ribosome_entry_site	1967	1979	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1899007	.	engineered_region	1967	1979	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	1973	1976	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	1967	1979	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	1255	1267	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1899007	.	engineered_region	1255	1267	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	1261	1264	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	1255	1267	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	75	87	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1899007	.	engineered_region	75	87	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	81	84	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1899007	.	ribosome_entry_site	75	87	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1899007	.	promoter	1	66	.	+	0	ID=BBa_K1899004;Name=BBa_K1899004
BBa_K1899007	.	CDS	94	1221	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1899007	.	CDS	94	1221	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1899007	.	engineered_tag	1222	1246	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1899007	.	engineered_region	94	1221	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1899007	.	tag	1183	1221	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1899007	.	CDS	1274	1958	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K1899007	.	tag	1894	1927	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1899007	.	engineered_tag	1934	1958	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1899007	.	CDS	1277	1893	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_K1899007
cgacgtacggtggaatctgattcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggttactagagtcaca
caggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagac
cgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctg
aattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctgg
ccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgat
ggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatt
aactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtct
ctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcatt
gggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaa
tatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaacca
tgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgc
cattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttat
atcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc
agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgca
aaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacacagga
aagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacagg
aaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaat
gggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcacta
ctggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccaga
tcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagat
gacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattg
attttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcaga
caaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccat
tatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgcccttt
cgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgga
tgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata