BBa_K1900003 1 BBa_K1900003 E. coli tolC 2016-10-07T11:00:00Z 2016-10-20T10:11:22Z This part comes from the E. coli K-12 tolC gene sequence. This is the E. coli K-12 tolC gene which produces the E. coli TolC protein. This protein is necessary for antibiotic efflux and was used as a control for our project. TolC proteins can be used to screen for bacteriophages, this is the central idea of our project. false false _2366_ 33079 33079 9 false For this design false Elise Sloey BBa_K1900002 1 BBa_K1900002 pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC 2016-10-11T11:00:00Z 2016-10-20T10:39:05Z This part This part false false _2366_ 33079 33079 9 false This part false Elise Sloey component2525440 1 BBa_K1406000 component2525433 1 BBa_K1900003 annotation2525433 1 BBa_K1900003 range2525433 1 1 1482 annotation2525440 1 BBa_K1406000 range2525440 1 1491 1641 BBa_K1406000 1 BBa_K1406000 pBAD promoter + strong RBS 2014-10-08T11:00:00Z 2015-05-08T01:10:17Z E. coli A combinatorial part of K206000, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pBAD promoter and the strong RBS using the restriction enzymes NheI and PstI. false false _1784_ 0 17066 9 It's complicated false We say we are hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick. false Sierra Tackett annotation2408503 1 promoter range2408503 1 1 131 annotation2408506 1 BBa_K206000 range2408506 1 1 130 annotation2408505 1 AraI2 range2408505 1 61 78 annotation2408504 1 AraI1 range2408504 1 40 57 annotation2408507 1 conserved range2408507 1 143 146 annotation2408508 1 BBa_B0034 range2408508 1 139 150 BBa_K1900003_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaatctgatgcaggtatatcagcaagcgcgtctgtccaatccggaactccgtaagagtgccgcagatcgtgatgccgcatttgaaaaaattaacgaagcgcgctccccgcttctgccgcaactgggcttgggagccgactacacctactctaacggctaccgtgacgcgaacggtattaacagcaacgcgacgtcggctagcctccaacttactcagagcatcttcgatatgtcgaaatggcgtgcattaactctgcaagaaaaagcggccggtattcaggacgtgacttatcagaccgaccaacaaacgttgattctgaacaccgccactgcttatttcaatgtgctgaacgctattgacgtactctcatacacccaggcgcagaaagaagctatttatcgccagctggaccagaccacccagcgctttaacgtcggcctggtcgcgatcacggatgttcagaacgcgcgcgcccaatatgatactgtgttggcgaatgaagtgaccgcacgcaacaatctggacaatgcggtagaacagctccgtcagattaccggaaactactacccggaactggccgctttgaatgtggaaaactttaaaaccgacaaaccgcagccagtgaacgcgctgctcaaagaagcggaaaaacgtaacctttcactgttacaagcccgcctgagccaggatttagcgcgtgagcaaattcgtcaggctcaggatgggcatctcccaacccttgatctgacagcaagcacgggcatcagtgacacctcctatagtggatcaaaaacacgcggcgcggccggcactcaatacgatgattccaatatgggccaaaataaagtgggcttaagcttcagcctgccgatttatcaaggtggaatggtgaactcccaggtcaaacaggctcagtataactttgtgggtgcgagcgagcaacttgaatcagcgcatcgttcagtagttcagactgtacgttcctcgtttaacaacatcaatgccagcatctcttcaatcaatgcctacaaacaagccgtggtctccgcccagagttcgctggatgccatggaagccggctattcggtaggcactcgcacgattgttgatgtactggatgcgacgacgaccctgtataacgcgaaacaggaactggcgaacgcgcgttataactatctgatcaaccaactgaatattaaatcggcgttaggtaccctgaacgagcaggatctgttggcgcttaataacgcactgagcaagccggtgagcacgaacccggaaaatgtggccccgcagaccccagaacaaaacgctattgcagatggctacgcgccagatagcccggctccggtagtacagcaaacctcagcccgcaccaccacctcaaacggccataacccgtttcgcaattaa BBa_K1406000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagc BBa_K1900002_sequence 1 atgaagaaattgctccccattcttatcggcctgagcctttctgggttcagttcgttgagccaggccgagaatctgatgcaggtatatcagcaagcgcgtctgtccaatccggaactccgtaagagtgccgcagatcgtgatgccgcatttgaaaaaattaacgaagcgcgctccccgcttctgccgcaactgggcttgggagccgactacacctactctaacggctaccgtgacgcgaacggtattaacagcaacgcgacgtcggctagcctccaacttactcagagcatcttcgatatgtcgaaatggcgtgcattaactctgcaagaaaaagcggccggtattcaggacgtgacttatcagaccgaccaacaaacgttgattctgaacaccgccactgcttatttcaatgtgctgaacgctattgacgtactctcatacacccaggcgcagaaagaagctatttatcgccagctggaccagaccacccagcgctttaacgtcggcctggtcgcgatcacggatgttcagaacgcgcgcgcccaatatgatactgtgttggcgaatgaagtgaccgcacgcaacaatctggacaatgcggtagaacagctccgtcagattaccggaaactactacccggaactggccgctttgaatgtggaaaactttaaaaccgacaaaccgcagccagtgaacgcgctgctcaaagaagcggaaaaacgtaacctttcactgttacaagcccgcctgagccaggatttagcgcgtgagcaaattcgtcaggctcaggatgggcatctcccaacccttgatctgacagcaagcacgggcatcagtgacacctcctatagtggatcaaaaacacgcggcgcggccggcactcaatacgatgattccaatatgggccaaaataaagtgggcttaagcttcagcctgccgatttatcaaggtggaatggtgaactcccaggtcaaacaggctcagtataactttgtgggtgcgagcgagcaacttgaatcagcgcatcgttcagtagttcagactgtacgttcctcgtttaacaacatcaatgccagcatctcttcaatcaatgcctacaaacaagccgtggtctccgcccagagttcgctggatgccatggaagccggctattcggtaggcactcgcacgattgttgatgtactggatgcgacgacgaccctgtataacgcgaaacaggaactggcgaacgcgcgttataactatctgatcaaccaactgaatattaaatcggcgttaggtaccctgaacgagcaggatctgttggcgcttaataacgcactgagcaagccggtgagcacgaacccggaaaatgtggccccgcagaccccagaacaaaacgctattgcagatggctacgcgccagatagcccggctccggtagtacagcaaacctcagcccgcaccaccacctcaaacggccataacccgtttcgcaattaatactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaaaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z