LOCUS BBa_K190026 6107 bp DNA linear 22-Nov-2024 DEFINITION GVP with medium constitutive promoter ACCESSION BBa_K190026 VERSION BBa_K190026.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 44..55 /label=b0034 CDS 62..328 /label=gvpB CDS 404..670 /label=gvpR CDS 699..1626 /label=gvpN CDS 1640..2407 /label=gvpF CDS 2407..2675 /label=gvpG CDS 2676..3885 /label=gvpL CDS 3903..4190 /label=gvpS CDS 4144..4428 /label=gvpK CDS 4450..4752 /label=gvpJ CDS 4789..5667 /label=gvpT CDS 5691..6107 /label=gvpU misc_feature 2888..2888 /label=gvpL g213a misc_feature 2993..2993 /label=gvpL g318a misc_feature 3026..3026 /label=gvpL g351a misc_feature 3371..3371 /label=gvpL g696a misc_feature 3387..3786 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 761..761 /label=c->a misc_feature 2746..2746 /label=t->c misc_feature 2815..2815 /label=t->c misc_feature 3265..3265 /label=t->a misc_feature 4575..4575 /label=t->a misc_feature 5113..5113 /label=g->a misc_feature 5176..5176 /label=g->a misc_feature 3347..3386 /label=First copy of 40bp section misc_feature 44..6107 /label=BBa_I750016 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtctatt caaaaaagta ctaatagttc aagtttagca gaagtcattg accgtatttt 121 agataaagga attgttattg atgcttttgc aagagtttct gttgtaggaa ttgaaatttt 181 aacgattgaa gcgcgagtgg ttattgccag tgttgataca tggttacgct atgcagaagc 241 agtagggctt cttcgtgacg acgtagaaga aaacggtctt cctgaacgtt caaattcaag 301 tgaagggcag ccgcgtttta gtatttaata tgaagtggta tggaaccttc cttttttgag 361 ggtggttcca gcttttagat ctaactattg gaggctacta aaaatggaaa ttaaaaaaat 421 tatgcaagcc gtgaacgact ttttcggtga acacgtagct cctcctcata aaattacctc 481 ggtggaagct actgaagatg aaggttggag agttattgtt gaagtcattg aagaacgaga 541 atatatgaaa aaatacgcca aagatgaaat gctcggaacg tacgagtgct ttgtaaataa 601 agaaaaagaa gtcatttcat tcaaacgact cgacgtcaga tatagaagcg ccattggcat 661 tgaagcataa acgataagat ggcaggagga acgtaaaaat gaccgtctta acagacaaaa 721 ggaaaaaagg cagtggagct tttatacaag atgacgagac aaaagaggtt ctttcaagag 781 cgctgagcta tttaaaatcc ggctattcca ttcattttac aggtcctgcc ggcggaggca 841 aaacctcttt agcgcgagcg cttgctaaaa agagaaagcg tcctgtaatg ctgatgcacg 901 ggaatcacga gctcaacaac aaagatttaa ttggcgattt tacgggatac acgagcaaaa 961 aagtaatcga ccagtacgtt cgttctgtct ataaaaaaga tgaacaggtg agtgaaaact 1021 ggcaggatgg ccgattgctt gaagctgtaa aaaatggcta tacgctgatt tacgacgaat 1081 ttactcgttc taagcctgcg acgaataata tctttctatc gatattagaa gaaggcgtgc 1141 tgccgctgta tggagtaaaa atgaccgatc cttttgtgcg cgtgcatccc gatttccgcg 1201 tcatcttcac aagcaatcca gctgagtatg ccggcgtata tgatacgcaa gatgcgcttc 1261 tcgacaggtt aattaccatg tttattgatt ataaagacat cgacagagag acagcgattt 1321 taacggagaa aacggacgta gaagaagatg aagcgcgcac aattgtaacg ctcgtagcaa 1381 acgtgcgaaa ccgctctgga gacgaaaaca gcagcggact tagcctgcgg gcttcgctta 1441 tgatcgctac ccttgccacg cagcaagaca ttcctatcga tggaagtgac gaagattttc 1501 aaacgttatg tatcgatatt ttgcatcatc cgcttaccaa atgtttggat gaagaaaatg 1561 caaaaagcaa agccgaaaaa atcattttag aagaatgtaa gaatatagac actgaagaaa 1621 agtaaaggag cttgaaaaca tgagtgaaac aaacgaaaca ggtatttata tttttagcgc 1681 cattcaaacg gataaagacg aagaatttgg cgccgtggaa gtagaaggaa caaaagctga 1741 aacatttttg attcgctaca aagacgcggc tatggtagca gctgaagtac cgatgaaaat 1801 ttatcatcct aatcgccaaa atttattaat gcatcaaaac gcagtagcag cgattatgga 1861 caagaacgat acggttattc caatcagctt tgggaatgta ttcaaatcaa aagaagacgt 1921 aaaagttctt ttggaaaacc tttatccgca gtttgaaaag ctgtttccag cgatcaaagg 1981 aaaaattgaa gtcggtttaa aagtaattgg gaaaaaagaa tggcttgaga aaaaagtaaa 2041 cgaaaatcct gaacttgaga aagtatcagc atccgtaaaa ggaaaatcag aagcagccgg 2101 ttattatgag cgtattcaac ttggaggaat ggctcaaaag atgtttactt ccctgcaaaa 2161 agaagtcaag acagatgtgt tttctccgct tgaagaagca gcggaagcag caaaagcaaa 2221 tgagccaacg ggcgaaacga tgcttttaaa cgcgtctttc ttaattaacc gagaagatga 2281 agcgaagttt gatgaaaaag taaatgaagc gcatgaaaac tggaaagaca aagccgattt 2341 tcattacagc ggtccttggc ctgcttataa ttttgtgaac attcgcctaa aagtagaaga 2401 gaaataacgt gcttcacaaa ttagtaaccg cacccattaa ccttgtagtg aaaatcggcg 2461 aaaaagtaca ggaagaagct gataaacagc tatatgacct tccgacgatt cagcaaaagc 2521 tcattcagct tcaaatgatg tttgagcttg gtgaaattcc agaagaagcg tttcaagaaa 2581 aagaagatga attgttaatg aggtacgaaa ttgcgaaacg cagagaaatt gaacaatggg 2641 aagagctaac acaaaaaaga aatgaggaat cctagatggg agaattactg tatttatacg 2701 gtttaattcc aacaaaagaa gcagcagcca tagagccgtt tccatcttat aaggggtttg 2761 acggagaaca ttcactgtac ccaattgcgt ttgatcaggt gacggctgta gtttctaagc 2821 tggatgctga cacctattca gaaaaagtga ttcaagaaaa aatggagcag gatatgagct 2881 ggctgcaaga aaaagcattt catcatcacg aaacggtagc cgctttgtac gaagaattta 2941 cgatcattcc attaaaattt tgcaccattt ataaaggtga agaaagtctg caagcagcta 3001 ttgagattaa caaagaaaag atagaaaatt cactgacgct gcttcaagga aatgaagagt 3061 ggaatgtgaa aatttactgt gatgatacag agcttaaaaa aggaatcagc gaaacgaatg 3121 aaagcgtgaa agcgaaaaaa caagaaatta gtcacttatc accaggaaga cagttttttg 3181 aaaagaaaaa aatagatcag ctgattgaaa aagaattaga gcttcacaaa aacaaagtgt 3241 gtgaagagat acatgacaag ctaaaagaat tatcgcttta tgactctgtt aaaaagaatt 3301 ggagcaaaga cgtaactggc gcagctgaac agatggcgtg gaacagcgtg tttcttctcc 3361 cgtctctgca aattactaag ttcgtacgtg tttcttctcc cgtctctgca aattactaag 3421 ttcgtacgtg tttcttctcc cgtctctgca aattactaag ttcgtacgtg tttcttctcc 3481 cgtctctgca aattactaag ttcgtacgtg tttcttctcc cgtctctgca aattactaag 3541 ttcgtacgtg tttcttctcc cgtctctgca aattactaag ttcgtacgtg tttcttctcc 3601 cgtctctgca aattactaag ttcgtacgtg tttcttctcc cgtctctgca aattactaag 3661 ttcgtacgtg tttcttctcc cgtctctgca aattactaag ttcgtacgtg tttcttctcc 3721 cgtctctgca aattactaag ttcgtacgtg tttcttctcc cgtctctgca aattactaag 3781 ttcgtaaacg aaatagaaga gcttcagcaa aggcttgaaa ataaaggctg gaagtttgaa 3841 gtgacgggac catggccgcc ctatcatttc tcgagctttg cgtaaagtga ggaattaaca 3901 ttatgtctct taaacaatcc atggagaata aagatattgc tcttattgat attttagatg 3961 tcattttaga taaaggagtc gccattaaag gagacttaat catttccata gctggcgtcg 4021 atttagtgta tttggatttg cgggtgctta tttcttcggt tgaaacgctt gtgcaagcaa 4081 aagaaggaaa tcacaaacca atcacttctg aacaatttga taaacaaaag gaggaattaa 4141 tggatgcaac cggtcagcca agcaaatgga cgaatccact tggatcctga tcaagctgaa 4201 caaggcttag cgcagcttgt gatgacagtt attgagctat tgaggcaaat cgttgaacgt 4261 catgccatga ggcgggtgga gggtggaacg ttgacggacg aacaaattga aaacttagga 4321 attgcactaa tgaacttaga agaaaaaatg gacgagttga aagaggtgtt cggtctggat 4381 gcagaagatt taaatattga tcttggaccg ctaggcagcc tgctttaagc ggtcagtagg 4441 aggaacagta tggcagtcga acataatatg cagtcaagta cgattgtaga tgtgctcgaa 4501 aagattttgg ataaaggagt cgttatagcg ggggacatca ccgtaggaat tgcagatgtc 4561 gagctattaa cgataaagat ccgcttgatt gtggcttcgg ttgataaggc aaaagaaatc 4621 ggcatggact ggtgggaaaa tgatccgtat ctcagttcaa aaggagccaa taacaaagcg 4681 ctcgaagaag aaaataaaat gctgcatgag cggttaaaaa cgcttgaaga aaaaatagaa 4741 acgaaacgtt aaaaactgta cgctacttaa aaaatggagg gatttacaat ggcaactgaa 4801 acaaaattag ataacacaca ggcagaaaac aaggaaaata aaaatgcgga aaacggttca 4861 aaagaaaaga acggttcaaa agcaagcaaa acaacaagca gcgggccaat caaacgagcg 4921 gtagcaggag gcatcatcgg tgcaacgatt ggatatgtat cgactcctga aaatcgaaaa 4981 agtctccttg accgcattga tacagacgaa ttaaaaagca aagcatctga tttaggaaca 5041 aaggtaaaag aaaaatcaaa aagcagcgtg gccagcctga aaacatctgc gggaagcttg 5101 tttaaaaaag ataaagataa atcaaaagat gatgaagaaa acgtaaattc ttctagtagc 5161 gaaacagaag acgataacgt tcaagagtac gacgagttaa aagaagaaaa tcaaactctt 5221 caagatcgct tatcacagct tgaagaaaaa atgaacatgc ttgttgagct tagcctcaat 5281 aaaaatcaag acgaagaagc ggaagataca gattccgacg aagaagagaa cgatgagaac 5341 gatgaaaacg atgaaaacga gcaggacgat gaaaacgaag aagaaacatc taagccacgt 5401 aaaaaggata aaaaagaagc tgaggaagaa gaaagtgaaa gtgacgaaga cagcgaggaa 5461 gaagaggaag attctcgctc aaacaaaaaa aataaaaaag taaaaacaga agaagaagac 5521 gaagatgaaa gcgaagaaga aaaaaaggaa gcgaaaccaa aaaagtcaac agctaaaaaa 5581 tcaaaaaata caaaagcaaa gaaaaacacg gacgaagaag atgatgaagc aacatctctt 5641 tctagtgaag acgatacaac agcctaagac gtaaaggagg aaagaaagac atgagtacag 5701 gcccttcttt ttcaactaaa gacaatacgc ttgaatactt tgtgaaagct tctaataaac 5761 acggcttttc acttgatatt tcattaaatg taaacggcgc tgtgatttcc ggtaccatga 5821 tttcagcaaa agaatacttt gattacttaa gcgaaacgtt tgaagaaggc agtgaagtgg 5881 ctcaggcgct aagcgaacaa ttctctttag caagcgaagc gagcgaatca aacggagaag 5941 cagaagccca ttttattcat ttgaaaaata caaagattta ctgtggagac agtaaatcta 6001 ctccttctaa aggcaaaatc ttttggagag ggaaaatagc agaagtagac gggtttttct 6061 taggaaagat ttctgatgca aaatcaacga gtaaaaagag ttcataa //