LOCUS BBa_K190033 6144 bp DNA linear 14-Nov-2024 DEFINITION GVP with ArsR regulated promoter ACCESSION BBa_K190033 VERSION BBa_K190033.1 FEATURES Location/Qualifiers misc_feature 9..41 /label=ArsR binding site promoter 38..72 /label=-35 and -10 region promoter 1..72 /label=BBa_K190015 RBS 81..92 /label=b0034 CDS 99..365 /label=gvpB CDS 441..707 /label=gvpR CDS 736..1663 /label=gvpN CDS 1677..2444 /label=gvpF CDS 2444..2712 /label=gvpG CDS 2713..3922 /label=gvpL CDS 3940..4227 /label=gvpS CDS 4181..4465 /label=gvpK CDS 4487..4789 /label=gvpJ CDS 4826..5704 /label=gvpT CDS 5728..6144 /label=gvpU misc_feature 2925..2925 /label=gvpL g213a misc_feature 3030..3030 /label=gvpL g318a misc_feature 3063..3063 /label=gvpL g351a misc_feature 3408..3408 /label=gvpL g696a misc_feature 3424..3823 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 798..798 /label=c->a misc_feature 2783..2783 /label=t->c misc_feature 2852..2852 /label=t->c misc_feature 3302..3302 /label=t->a misc_feature 4612..4612 /label=t->a misc_feature 5150..5150 /label=g->a misc_feature 5213..5213 /label=g->a misc_feature 3384..3423 /label=First copy of 40bp section misc_feature 81..6144 /label=BBa_I750016 ORIGIN 1 ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc gaagagagac 61 actacctgca attactagag aaagaggaga aatactagat gtctattcaa aaaagtacta 121 atagttcaag tttagcagaa gtcattgacc gtattttaga taaaggaatt gttattgatg 181 cttttgcaag agtttctgtt gtaggaattg aaattttaac gattgaagcg cgagtggtta 241 ttgccagtgt tgatacatgg ttacgctatg cagaagcagt agggcttctt cgtgacgacg 301 tagaagaaaa cggtcttcct gaacgttcaa attcaagtga agggcagccg cgttttagta 361 tttaatatga agtggtatgg aaccttcctt ttttgagggt ggttccagct tttagatcta 421 actattggag gctactaaaa atggaaatta aaaaaattat gcaagccgtg aacgactttt 481 tcggtgaaca cgtagctcct cctcataaaa ttacctcggt ggaagctact gaagatgaag 541 gttggagagt tattgttgaa gtcattgaag aacgagaata tatgaaaaaa tacgccaaag 601 atgaaatgct cggaacgtac gagtgctttg taaataaaga aaaagaagtc atttcattca 661 aacgactcga cgtcagatat agaagcgcca ttggcattga agcataaacg ataagatggc 721 aggaggaacg taaaaatgac cgtcttaaca gacaaaagga aaaaaggcag tggagctttt 781 atacaagatg acgagacaaa agaggttctt tcaagagcgc tgagctattt aaaatccggc 841 tattccattc attttacagg tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt 901 gctaaaaaga gaaagcgtcc tgtaatgctg atgcacggga atcacgagct caacaacaaa 961 gatttaattg gcgattttac gggatacacg agcaaaaaag taatcgacca gtacgttcgt 1021 tctgtctata aaaaagatga acaggtgagt gaaaactggc aggatggccg attgcttgaa 1081 gctgtaaaaa atggctatac gctgatttac gacgaattta ctcgttctaa gcctgcgacg 1141 aataatatct ttctatcgat attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg 1201 accgatcctt ttgtgcgcgt gcatcccgat ttccgcgtca tcttcacaag caatccagct 1261 gagtatgccg gcgtatatga tacgcaagat gcgcttctcg acaggttaat taccatgttt 1321 attgattata aagacatcga cagagagaca gcgattttaa cggagaaaac ggacgtagaa 1381 gaagatgaag cgcgcacaat tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac 1441 gaaaacagca gcggacttag cctgcgggct tcgcttatga tcgctaccct tgccacgcag 1501 caagacattc ctatcgatgg aagtgacgaa gattttcaaa cgttatgtat cgatattttg 1561 catcatccgc ttaccaaatg tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc 1621 attttagaag aatgtaagaa tatagacact gaagaaaagt aaaggagctt gaaaacatga 1681 gtgaaacaaa cgaaacaggt atttatattt ttagcgccat tcaaacggat aaagacgaag 1741 aatttggcgc cgtggaagta gaaggaacaa aagctgaaac atttttgatt cgctacaaag 1801 acgcggctat ggtagcagct gaagtaccga tgaaaattta tcatcctaat cgccaaaatt 1861 tattaatgca tcaaaacgca gtagcagcga ttatggacaa gaacgatacg gttattccaa 1921 tcagctttgg gaatgtattc aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt 1981 atccgcagtt tgaaaagctg tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag 2041 taattgggaa aaaagaatgg cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag 2101 tatcagcatc cgtaaaagga aaatcagaag cagccggtta ttatgagcgt attcaacttg 2161 gaggaatggc tcaaaagatg tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt 2221 ctccgcttga agaagcagcg gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc 2281 ttttaaacgc gtctttctta attaaccgag aagatgaagc gaagtttgat gaaaaagtaa 2341 atgaagcgca tgaaaactgg aaagacaaag ccgattttca ttacagcggt ccttggcctg 2401 cttataattt tgtgaacatt cgcctaaaag tagaagagaa ataacgtgct tcacaaatta 2461 gtaaccgcac ccattaacct tgtagtgaaa atcggcgaaa aagtacagga agaagctgat 2521 aaacagctat atgaccttcc gacgattcag caaaagctca ttcagcttca aatgatgttt 2581 gagcttggtg aaattccaga agaagcgttt caagaaaaag aagatgaatt gttaatgagg 2641 tacgaaattg cgaaacgcag agaaattgaa caatgggaag agctaacaca aaaaagaaat 2701 gaggaatcct agatgggaga attactgtat ttatacggtt taattccaac aaaagaagca 2761 gcagccatag agccgtttcc atcttataag gggtttgacg gagaacattc actgtaccca 2821 attgcgtttg atcaggtgac ggctgtagtt tctaagctgg atgctgacac ctattcagaa 2881 aaagtgattc aagaaaaaat ggagcaggat atgagctggc tgcaagaaaa agcatttcat 2941 catcacgaaa cggtagccgc tttgtacgaa gaatttacga tcattccatt aaaattttgc 3001 accatttata aaggtgaaga aagtctgcaa gcagctattg agattaacaa agaaaagata 3061 gaaaattcac tgacgctgct tcaaggaaat gaagagtgga atgtgaaaat ttactgtgat 3121 gatacagagc ttaaaaaagg aatcagcgaa acgaatgaaa gcgtgaaagc gaaaaaacaa 3181 gaaattagtc acttatcacc aggaagacag ttttttgaaa agaaaaaaat agatcagctg 3241 attgaaaaag aattagagct tcacaaaaac aaagtgtgtg aagagataca tgacaagcta 3301 aaagaattat cgctttatga ctctgttaaa aagaattgga gcaaagacgt aactggcgca 3361 gctgaacaga tggcgtggaa cagcgtgttt cttctcccgt ctctgcaaat tactaagttc 3421 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3481 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3541 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3601 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3661 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3721 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3781 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtaaacgaaa tagaagagct 3841 tcagcaaagg cttgaaaata aaggctggaa gtttgaagtg acgggaccat ggccgcccta 3901 tcatttctcg agctttgcgt aaagtgagga attaacatta tgtctcttaa acaatccatg 3961 gagaataaag atattgctct tattgatatt ttagatgtca ttttagataa aggagtcgcc 4021 attaaaggag acttaatcat ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg 4081 gtgcttattt cttcggttga aacgcttgtg caagcaaaag aaggaaatca caaaccaatc 4141 acttctgaac aatttgataa acaaaaggag gaattaatgg atgcaaccgg tcagccaagc 4201 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat 4261 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg 4321 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga 4381 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct 4441 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca 4501 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt 4561 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg 4621 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga 4681 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct 4741 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc 4801 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc 4861 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc 4921 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc 4981 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac 5041 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag 5101 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc 5161 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca 5221 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga 5281 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga 5341 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca 5401 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga 5461 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa 5521 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa 5581 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa 5641 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc 5701 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac 5761 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca 5821 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat 5881 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc 5941 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg 6001 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt 6061 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa 6121 tcaacgagta aaaagagttc ataa //