##gff-version 3
##sequence-region BBa_K190033 1 6144
BBa_K190033	.	engineered_region	81	6144	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_K190033	.	CDS	4487	4789	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_K190033	.	sequence_feature	3424	3823	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_K190033	.	sequence_feature	3384	3423	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_K190033	.	CDS	2713	3922	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_K190033	.	point_mutation	2852	2852	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_K190033	.	point_mutation	3408	3408	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_K190033	.	CDS	5728	6144	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_K190033	.	CDS	99	365	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_K190033	.	CDS	2444	2712	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
BBa_K190033	.	CDS	1677	2444	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
BBa_K190033	.	CDS	736	1663	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_K190033	.	sequence_alteration	3302	3302	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	2925	2925	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	5150	5150	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	3030	3030	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_K190033	.	CDS	4181	4465	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
BBa_K190033	.	CDS	4826	5704	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_K190033	.	CDS	441	707	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_K190033	.	ribosome_entry_site	81	92	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_K190033	.	point_mutation	2783	2783	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_K190033	.	point_mutation	5213	5213	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	3063	3063	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	798	798	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_K190033	.	point_mutation	4612	4612	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_K190033	.	CDS	3940	4227	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_K190033	.	promoter	1	72	.	+	0	ID=BBa_K190015;Name=BBa_K190015
BBa_K190033	.	promoter	38	72	.	+	0	ID=annotation2011502;Name=-35 and -10 region;Parent=BBa_K190015
BBa_K190033	.	non_covalent_binding_site	9	41	.	+	0	ID=annotation2011501;Name=ArsR binding site;Parent=BBa_K190015
>BBa_K190033
ctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaattactaga
gaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtatttta
gataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtgg
ttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtct
tcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttg
agggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacg
actttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttat
tgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgta
aataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacga
taagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatg
acgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccgg
cggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgag
ctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtct
ataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgat
ttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctg
tatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagt
atgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacag
agagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtg
cgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagc
aagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatg
tttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaag
taaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacga
agaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagca
gctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatgg
acaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaa
cctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaa
gaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcag
ccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacaga
tgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcg
tctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaag
ccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgc
ttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagct
atatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcg
tttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaa
cacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagc
catagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggct
gtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgc
aagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcac
catttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgctt
caaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaa
gcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagct
gattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttat
gactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcc
cgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctc
ccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttct
cccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttc
tcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttctt
ctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttct
tctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaag
tttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaa
acaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaagga
gacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttg
tgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaac
cggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagt
tattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaatt
gaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaag
atttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataa
tatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgta
ggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggca
tggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgct
gcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggaggga
tttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaa
aagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcgg
tgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagc
aaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagct
tgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataa
cgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaac
atgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatg
agaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaa
agaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaat
aaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacag
ctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaaga
cgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgc
ttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttc
cggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcg
ctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaa
atacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagt
agacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataa