LOCUS BBa_K190034 6141 bp DNA linear 14-Nov-2024 DEFINITION GVP with ZntR regulated promoter ACCESSION BBa_K190034 VERSION BBa_K190034.1 FEATURES Location/Qualifiers promoter 35..69 /label=-35 and -10 region misc_feature 32..53 /label=Palindromic binding site for ZntR promoter 1..69 /label=BBa_K190016 RBS 78..89 /label=b0034 CDS 96..362 /label=gvpB CDS 438..704 /label=gvpR CDS 733..1660 /label=gvpN CDS 1674..2441 /label=gvpF CDS 2441..2709 /label=gvpG CDS 2710..3919 /label=gvpL CDS 3937..4224 /label=gvpS CDS 4178..4462 /label=gvpK CDS 4484..4786 /label=gvpJ CDS 4823..5701 /label=gvpT CDS 5725..6141 /label=gvpU misc_feature 2922..2922 /label=gvpL g213a misc_feature 3027..3027 /label=gvpL g318a misc_feature 3060..3060 /label=gvpL g351a misc_feature 3405..3405 /label=gvpL g696a misc_feature 3421..3820 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 795..795 /label=c->a misc_feature 2780..2780 /label=t->c misc_feature 2849..2849 /label=t->c misc_feature 3299..3299 /label=t->a misc_feature 4609..4609 /label=t->a misc_feature 5147..5147 /label=g->a misc_feature 5210..5210 /label=g->a misc_feature 3381..3420 /label=First copy of 40bp section misc_feature 78..6141 /label=BBa_I750016 ORIGIN 1 cgtccgctcg ctgtatctct gataaaactt gactctggag tcgactccag agtgtatcct 61 tcggttaatt actagagaaa gaggagaaat actagatgtc tattcaaaaa agtactaata 121 gttcaagttt agcagaagtc attgaccgta ttttagataa aggaattgtt attgatgctt 181 ttgcaagagt ttctgttgta ggaattgaaa ttttaacgat tgaagcgcga gtggttattg 241 ccagtgttga tacatggtta cgctatgcag aagcagtagg gcttcttcgt gacgacgtag 301 aagaaaacgg tcttcctgaa cgttcaaatt caagtgaagg gcagccgcgt tttagtattt 361 aatatgaagt ggtatggaac cttccttttt tgagggtggt tccagctttt agatctaact 421 attggaggct actaaaaatg gaaattaaaa aaattatgca agccgtgaac gactttttcg 481 gtgaacacgt agctcctcct cataaaatta cctcggtgga agctactgaa gatgaaggtt 541 ggagagttat tgttgaagtc attgaagaac gagaatatat gaaaaaatac gccaaagatg 601 aaatgctcgg aacgtacgag tgctttgtaa ataaagaaaa agaagtcatt tcattcaaac 661 gactcgacgt cagatataga agcgccattg gcattgaagc ataaacgata agatggcagg 721 aggaacgtaa aaatgaccgt cttaacagac aaaaggaaaa aaggcagtgg agcttttata 781 caagatgacg agacaaaaga ggttctttca agagcgctga gctatttaaa atccggctat 841 tccattcatt ttacaggtcc tgccggcgga ggcaaaacct ctttagcgcg agcgcttgct 901 aaaaagagaa agcgtcctgt aatgctgatg cacgggaatc acgagctcaa caacaaagat 961 ttaattggcg attttacggg atacacgagc aaaaaagtaa tcgaccagta cgttcgttct 1021 gtctataaaa aagatgaaca ggtgagtgaa aactggcagg atggccgatt gcttgaagct 1081 gtaaaaaatg gctatacgct gatttacgac gaatttactc gttctaagcc tgcgacgaat 1141 aatatctttc tatcgatatt agaagaaggc gtgctgccgc tgtatggagt aaaaatgacc 1201 gatccttttg tgcgcgtgca tcccgatttc cgcgtcatct tcacaagcaa tccagctgag 1261 tatgccggcg tatatgatac gcaagatgcg cttctcgaca ggttaattac catgtttatt 1321 gattataaag acatcgacag agagacagcg attttaacgg agaaaacgga cgtagaagaa 1381 gatgaagcgc gcacaattgt aacgctcgta gcaaacgtgc gaaaccgctc tggagacgaa 1441 aacagcagcg gacttagcct gcgggcttcg cttatgatcg ctacccttgc cacgcagcaa 1501 gacattccta tcgatggaag tgacgaagat tttcaaacgt tatgtatcga tattttgcat 1561 catccgctta ccaaatgttt ggatgaagaa aatgcaaaaa gcaaagccga aaaaatcatt 1621 ttagaagaat gtaagaatat agacactgaa gaaaagtaaa ggagcttgaa aacatgagtg 1681 aaacaaacga aacaggtatt tatattttta gcgccattca aacggataaa gacgaagaat 1741 ttggcgccgt ggaagtagaa ggaacaaaag ctgaaacatt tttgattcgc tacaaagacg 1801 cggctatggt agcagctgaa gtaccgatga aaatttatca tcctaatcgc caaaatttat 1861 taatgcatca aaacgcagta gcagcgatta tggacaagaa cgatacggtt attccaatca 1921 gctttgggaa tgtattcaaa tcaaaagaag acgtaaaagt tcttttggaa aacctttatc 1981 cgcagtttga aaagctgttt ccagcgatca aaggaaaaat tgaagtcggt ttaaaagtaa 2041 ttgggaaaaa agaatggctt gagaaaaaag taaacgaaaa tcctgaactt gagaaagtat 2101 cagcatccgt aaaaggaaaa tcagaagcag ccggttatta tgagcgtatt caacttggag 2161 gaatggctca aaagatgttt acttccctgc aaaaagaagt caagacagat gtgttttctc 2221 cgcttgaaga agcagcggaa gcagcaaaag caaatgagcc aacgggcgaa acgatgcttt 2281 taaacgcgtc tttcttaatt aaccgagaag atgaagcgaa gtttgatgaa aaagtaaatg 2341 aagcgcatga aaactggaaa gacaaagccg attttcatta cagcggtcct tggcctgctt 2401 ataattttgt gaacattcgc ctaaaagtag aagagaaata acgtgcttca caaattagta 2461 accgcaccca ttaaccttgt agtgaaaatc ggcgaaaaag tacaggaaga agctgataaa 2521 cagctatatg accttccgac gattcagcaa aagctcattc agcttcaaat gatgtttgag 2581 cttggtgaaa ttccagaaga agcgtttcaa gaaaaagaag atgaattgtt aatgaggtac 2641 gaaattgcga aacgcagaga aattgaacaa tgggaagagc taacacaaaa aagaaatgag 2701 gaatcctaga tgggagaatt actgtattta tacggtttaa ttccaacaaa agaagcagca 2761 gccatagagc cgtttccatc ttataagggg tttgacggag aacattcact gtacccaatt 2821 gcgtttgatc aggtgacggc tgtagtttct aagctggatg ctgacaccta ttcagaaaaa 2881 gtgattcaag aaaaaatgga gcaggatatg agctggctgc aagaaaaagc atttcatcat 2941 cacgaaacgg tagccgcttt gtacgaagaa tttacgatca ttccattaaa attttgcacc 3001 atttataaag gtgaagaaag tctgcaagca gctattgaga ttaacaaaga aaagatagaa 3061 aattcactga cgctgcttca aggaaatgaa gagtggaatg tgaaaattta ctgtgatgat 3121 acagagctta aaaaaggaat cagcgaaacg aatgaaagcg tgaaagcgaa aaaacaagaa 3181 attagtcact tatcaccagg aagacagttt tttgaaaaga aaaaaataga tcagctgatt 3241 gaaaaagaat tagagcttca caaaaacaaa gtgtgtgaag agatacatga caagctaaaa 3301 gaattatcgc tttatgactc tgttaaaaag aattggagca aagacgtaac tggcgcagct 3361 gaacagatgg cgtggaacag cgtgtttctt ctcccgtctc tgcaaattac taagttcgta 3421 cgtgtttctt ctcccgtctc tgcaaattac taagttcgta cgtgtttctt ctcccgtctc 3481 tgcaaattac taagttcgta cgtgtttctt ctcccgtctc tgcaaattac taagttcgta 3541 cgtgtttctt ctcccgtctc tgcaaattac taagttcgta cgtgtttctt ctcccgtctc 3601 tgcaaattac taagttcgta cgtgtttctt ctcccgtctc tgcaaattac taagttcgta 3661 cgtgtttctt ctcccgtctc tgcaaattac taagttcgta cgtgtttctt ctcccgtctc 3721 tgcaaattac taagttcgta cgtgtttctt ctcccgtctc tgcaaattac taagttcgta 3781 cgtgtttctt ctcccgtctc tgcaaattac taagttcgta aacgaaatag aagagcttca 3841 gcaaaggctt gaaaataaag gctggaagtt tgaagtgacg ggaccatggc cgccctatca 3901 tttctcgagc tttgcgtaaa gtgaggaatt aacattatgt ctcttaaaca atccatggag 3961 aataaagata ttgctcttat tgatatttta gatgtcattt tagataaagg agtcgccatt 4021 aaaggagact taatcatttc catagctggc gtcgatttag tgtatttgga tttgcgggtg 4081 cttatttctt cggttgaaac gcttgtgcaa gcaaaagaag gaaatcacaa accaatcact 4141 tctgaacaat ttgataaaca aaaggaggaa ttaatggatg caaccggtca gccaagcaaa 4201 tggacgaatc cacttggatc ctgatcaagc tgaacaaggc ttagcgcagc ttgtgatgac 4261 agttattgag ctattgaggc aaatcgttga acgtcatgcc atgaggcggg tggagggtgg 4321 aacgttgacg gacgaacaaa ttgaaaactt aggaattgca ctaatgaact tagaagaaaa 4381 aatggacgag ttgaaagagg tgttcggtct ggatgcagaa gatttaaata ttgatcttgg 4441 accgctaggc agcctgcttt aagcggtcag taggaggaac agtatggcag tcgaacataa 4501 tatgcagtca agtacgattg tagatgtgct cgaaaagatt ttggataaag gagtcgttat 4561 agcgggggac atcaccgtag gaattgcaga tgtcgagcta ttaacgataa agatccgctt 4621 gattgtggct tcggttgata aggcaaaaga aatcggcatg gactggtggg aaaatgatcc 4681 gtatctcagt tcaaaaggag ccaataacaa agcgctcgaa gaagaaaata aaatgctgca 4741 tgagcggtta aaaacgcttg aagaaaaaat agaaacgaaa cgttaaaaac tgtacgctac 4801 ttaaaaaatg gagggattta caatggcaac tgaaacaaaa ttagataaca cacaggcaga 4861 aaacaaggaa aataaaaatg cggaaaacgg ttcaaaagaa aagaacggtt caaaagcaag 4921 caaaacaaca agcagcgggc caatcaaacg agcggtagca ggaggcatca tcggtgcaac 4981 gattggatat gtatcgactc ctgaaaatcg aaaaagtctc cttgaccgca ttgatacaga 5041 cgaattaaaa agcaaagcat ctgatttagg aacaaaggta aaagaaaaat caaaaagcag 5101 cgtggccagc ctgaaaacat ctgcgggaag cttgtttaaa aaagataaag ataaatcaaa 5161 agatgatgaa gaaaacgtaa attcttctag tagcgaaaca gaagacgata acgttcaaga 5221 gtacgacgag ttaaaagaag aaaatcaaac tcttcaagat cgcttatcac agcttgaaga 5281 aaaaatgaac atgcttgttg agcttagcct caataaaaat caagacgaag aagcggaaga 5341 tacagattcc gacgaagaag agaacgatga gaacgatgaa aacgatgaaa acgagcagga 5401 cgatgaaaac gaagaagaaa catctaagcc acgtaaaaag gataaaaaag aagctgagga 5461 agaagaaagt gaaagtgacg aagacagcga ggaagaagag gaagattctc gctcaaacaa 5521 aaaaaataaa aaagtaaaaa cagaagaaga agacgaagat gaaagcgaag aagaaaaaaa 5581 ggaagcgaaa ccaaaaaagt caacagctaa aaaatcaaaa aatacaaaag caaagaaaaa 5641 cacggacgaa gaagatgatg aagcaacatc tctttctagt gaagacgata caacagccta 5701 agacgtaaag gaggaaagaa agacatgagt acaggccctt ctttttcaac taaagacaat 5761 acgcttgaat actttgtgaa agcttctaat aaacacggct tttcacttga tatttcatta 5821 aatgtaaacg gcgctgtgat ttccggtacc atgatttcag caaaagaata ctttgattac 5881 ttaagcgaaa cgtttgaaga aggcagtgaa gtggctcagg cgctaagcga acaattctct 5941 ttagcaagcg aagcgagcga atcaaacgga gaagcagaag cccattttat tcatttgaaa 6001 aatacaaaga tttactgtgg agacagtaaa tctactcctt ctaaaggcaa aatcttttgg 6061 agagggaaaa tagcagaagt agacgggttt ttcttaggaa agatttctga tgcaaaatca 6121 acgagtaaaa agagttcata a //