##gff-version 3
##sequence-region BBa_K190034 1 6141
BBa_K190034	.	promoter	1	69	.	+	0	ID=BBa_K190016;Name=BBa_K190016
BBa_K190034	.	promoter	35	69	.	+	0	ID=annotation2011503;Name=-35 and -10 region;Parent=BBa_K190016
BBa_K190034	.	non_covalent_binding_site	32	53	.	+	0	ID=annotation2011504;Name=Palindromic binding site for ZntR;Parent=BBa_K190016
BBa_K190034	.	engineered_region	78	6141	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_K190034	.	CDS	4178	4462	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
BBa_K190034	.	CDS	438	704	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_K190034	.	CDS	733	1660	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_K190034	.	CDS	2710	3919	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_K190034	.	point_mutation	3405	3405	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_K190034	.	point_mutation	3027	3027	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_K190034	.	sequence_alteration	3299	3299	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_K190034	.	sequence_feature	3381	3420	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_K190034	.	CDS	5725	6141	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_K190034	.	CDS	4823	5701	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_K190034	.	sequence_feature	3421	3820	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_K190034	.	point_mutation	3060	3060	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_K190034	.	CDS	3937	4224	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_K190034	.	point_mutation	2780	2780	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_K190034	.	CDS	4484	4786	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_K190034	.	point_mutation	2922	2922	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_K190034	.	CDS	2441	2709	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
BBa_K190034	.	point_mutation	795	795	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_K190034	.	point_mutation	5210	5210	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_K190034	.	point_mutation	2849	2849	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_K190034	.	point_mutation	4609	4609	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_K190034	.	CDS	96	362	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_K190034	.	point_mutation	5147	5147	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_K190034	.	ribosome_entry_site	78	89	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_K190034	.	CDS	1674	2441	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
>BBa_K190034
cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaattactagagaa
agaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagat
aaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggtta
ttgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcc
tgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagg
gtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgact
ttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgt
tgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaat
aaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataa
gatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacg
agacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcgg
aggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctc
aacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctata
aaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgattta
cgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtat
ggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatg
ccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagaga
gacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcga
aaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaag
acattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgttt
ggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaa
aggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaaga
atttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagct
gaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggaca
agaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacct
ttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaa
tggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccg
gttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgt
gttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtct
ttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccg
attttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttc
acaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctata
tgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgttt
caagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacac
aaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccat
agagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgta
gtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaag
aaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccat
ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaa
ggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcg
tgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgat
tgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgac
tctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgt
ctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccg
tctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctccc
gtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcc
cgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctc
ccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttct
cccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagttt
gaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaaca
atccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagac
ttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgc
aagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccgg
tcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttat
tgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaa
aacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatt
taaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatat
gcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtagga
attgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatgg
actggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgca
tgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggattt
acaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaag
aaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgc
aacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaa
gcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgt
ttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgt
tcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatg
cttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgaga
acgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaaga
agctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaa
aaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagcta
aaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacga
tacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttg
aatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccgg
taccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgcta
agcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaata
caaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtaga
cgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataa