BBa_K190034
type: ComponentDefinition
persistent identity: BBa K190034
display id: BBa_K190034
version: 1
was generated by: iGEM to SBOL conversion
description: GVP with ZntR regulated promoter
created: 2009-09-06T11:00:00Z
modified: 2015-05-08T01:11:15Z
mutable provenance: E.coli and Bacillus
owned by: james
owned by: myers
mutable description: The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190016 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Melbourne GVP cluster was used containing 400bp repeat in gvpL
star: false
creator: Michael Verhoeven
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I750016
component: BBa_K190016
sequence annotation: BBa_K190016
sequence annotation: BBa_I750016
sequence: https://synbiohub.org/public/igem/BBa_K190034_sequence/1

BBa_I750016
type: Component
persistent identity: component2019573
display id: component2019573
version: 1
top level: BBa_K190034
definition: BBa_I750016
access: public

BBa_K190016
type: Component
persistent identity: component2019547
display id: component2019547
version: 1
top level: BBa_K190034
definition: BBa_K190016
access: public

BBa_K190016
type: SequenceAnnotation
persistent identity: annotation2019547
display id: annotation2019547
version: 1
top level: BBa_K190034
location: https://synbiohub.org/public/igem/BBa_K190034/annotation2019547/range2019547/1
component: BBa_K190016

https://synbiohub.org/public/igem/BBa_K190034/annotation2019547/range2019547/1
type: Range
persistent identity: range2019547
display id: range2019547
version: 1
top level: BBa_K190034
direction: direction/reverse
start: 1
end: 69
orientation: inline

BBa_I750016
type: SequenceAnnotation
persistent identity: annotation2019573
display id: annotation2019573
version: 1
top level: BBa_K190034
location: https://synbiohub.org/public/igem/BBa_K190034/annotation2019573/range2019573/1
component: BBa_I750016

https://synbiohub.org/public/igem/BBa_K190034/annotation2019573/range2019573/1
type: Range
persistent identity: range2019573
display id: range2019573
version: 1
top level: BBa_K190034
direction: direction/reverse
start: 78
end: 6141
orientation: inline

BBa_I750016
type: ComponentDefinition
persistent identity: BBa I750016
display id: BBa_I750016
version: 1
was generated by: iGEM to SBOL conversion
description: Gas Vesicle polycistonic gene
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458).
owned by: james
owned by: myers
mutable description: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.
discontinued: false
dominant: false
experience: experience/None
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part.
star: true
creator: Phillip Dodson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: b0034
sequence annotation: gvpB
sequence annotation: gvpR
sequence annotation: gvpN
sequence annotation: c->a
sequence annotation: gvpF
sequence annotation: gvpG
sequence annotation: gvpL
sequence annotation: t->c
sequence annotation: gvpL g213a
sequence annotation: gvpL g318a
sequence annotation: gvpL g351a
sequence annotation: t->a
sequence annotation: First copy of 40bp section
sequence annotation: gvpL g696a
sequence annotation: 10 repeats of 40bp induced durring mutagenesis
sequence annotation: gvpS
sequence annotation: gvpK
sequence annotation: gvpJ
sequence annotation: gvpT
sequence annotation: g->a
sequence annotation: gvpU
sequence: https://synbiohub.org/public/igem/BBa_I750016_sequence/1

b0034
type: SequenceAnnotation
persistent identity: annotation1951682
display id: annotation1951682
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
type: Range
persistent identity: range1951682
display id: range1951682
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1
end: 12
orientation: inline

gvpB
type: SequenceAnnotation
persistent identity: annotation1951683
display id: annotation1951683
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
type: Range
persistent identity: range1951683
display id: range1951683
version: 1
top level: BBa_I750016
direction: direction/forward
start: 19
end: 285
orientation: inline

gvpR
type: SequenceAnnotation
persistent identity: annotation1951684
display id: annotation1951684
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
type: Range
persistent identity: range1951684
display id: range1951684
version: 1
top level: BBa_I750016
direction: direction/forward
start: 361
end: 627
orientation: inline

gvpN
type: SequenceAnnotation
persistent identity: annotation1951685
display id: annotation1951685
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
type: Range
persistent identity: range1951685
display id: range1951685
version: 1
top level: BBa_I750016
direction: direction/forward
start: 656
end: 1583
orientation: inline

c->a
type: SequenceAnnotation
persistent identity: annotation1954599
display id: annotation1954599
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
type: Range
persistent identity: range1954599
display id: range1954599
version: 1
top level: BBa_I750016
direction: direction/forward
start: 718
end: 718
orientation: inline

gvpF
type: SequenceAnnotation
persistent identity: annotation1951686
display id: annotation1951686
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
type: Range
persistent identity: range1951686
display id: range1951686
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1597
end: 2364
orientation: inline

gvpG
type: SequenceAnnotation
persistent identity: annotation1951687
display id: annotation1951687
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
type: Range
persistent identity: range1951687
display id: range1951687
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2364
end: 2632
orientation: inline

gvpL
type: SequenceAnnotation
persistent identity: annotation1951688
display id: annotation1951688
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
type: Range
persistent identity: range1951688
display id: range1951688
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2633
end: 3842
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954600
display id: annotation1954600
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
type: Range
persistent identity: range1954600
display id: range1954600
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2703
end: 2703
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954601
display id: annotation1954601
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
type: Range
persistent identity: range1954601
display id: range1954601
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2772
end: 2772
orientation: inline

gvpL g213a
type: SequenceAnnotation
persistent identity: annotation1951694
display id: annotation1951694
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
type: Range
persistent identity: range1951694
display id: range1951694
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2845
end: 2845
orientation: inline

gvpL g318a
type: SequenceAnnotation
persistent identity: annotation1951696
display id: annotation1951696
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
type: Range
persistent identity: range1951696
display id: range1951696
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2950
end: 2950
orientation: inline

gvpL g351a
type: SequenceAnnotation
persistent identity: annotation1951697
display id: annotation1951697
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
type: Range
persistent identity: range1951697
display id: range1951697
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2983
end: 2983
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954602
display id: annotation1954602
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
type: Range
persistent identity: range1954602
display id: range1954602
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3222
end: 3222
orientation: inline

First copy of 40bp section
type: SequenceAnnotation
persistent identity: annotation1954616
display id: annotation1954616
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
type: Range
persistent identity: range1954616
display id: range1954616
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3304
end: 3343
orientation: inline

gvpL g696a
type: SequenceAnnotation
persistent identity: annotation1951698
display id: annotation1951698
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
type: Range
persistent identity: range1951698
display id: range1951698
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3328
end: 3328
orientation: inline

10 repeats of 40bp induced durring mutagenesis
type: SequenceAnnotation
persistent identity: annotation1954598
display id: annotation1954598
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
type: Range
persistent identity: range1954598
display id: range1954598
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3344
end: 3743
orientation: inline

gvpS
type: SequenceAnnotation
persistent identity: annotation1951689
display id: annotation1951689
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
type: Range
persistent identity: range1951689
display id: range1951689
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3860
end: 4147
orientation: inline

gvpK
type: SequenceAnnotation
persistent identity: annotation1951690
display id: annotation1951690
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
type: Range
persistent identity: range1951690
display id: range1951690
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4101
end: 4385
orientation: inline

gvpJ
type: SequenceAnnotation
persistent identity: annotation1951691
display id: annotation1951691
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
type: Range
persistent identity: range1951691
display id: range1951691
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4407
end: 4709
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954603
display id: annotation1954603
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
type: Range
persistent identity: range1954603
display id: range1954603
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4532
end: 4532
orientation: inline

gvpT
type: SequenceAnnotation
persistent identity: annotation1951692
display id: annotation1951692
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
type: Range
persistent identity: range1951692
display id: range1951692
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4746
end: 5624
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954604
display id: annotation1954604
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
type: Range
persistent identity: range1954604
display id: range1954604
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5070
end: 5070
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954605
display id: annotation1954605
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
type: Range
persistent identity: range1954605
display id: range1954605
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5133
end: 5133
orientation: inline

gvpU
type: SequenceAnnotation
persistent identity: annotation1951693
display id: annotation1951693
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
type: Range
persistent identity: range1951693
display id: range1951693
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5648
end: 6064
orientation: inline

BBa_K190016
type: ComponentDefinition
persistent identity: BBa K190016
display id: BBa_K190016
version: 1
was generated by: iGEM to SBOL conversion
description: Zinc Promoter (ZntR regulated)
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
mutable description: Promoter sequence with recognition site for ZntR transcriptional regulator protein.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Michael Verhoeven
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Palindromic binding site for ZntR
sequence annotation: -35 and -10 region
sequence: https://synbiohub.org/public/igem/BBa_K190016_sequence/1

Palindromic binding site for ZntR
type: SequenceAnnotation
persistent identity: annotation2011504
display id: annotation2011504
version: 1
top level: BBa_K190016
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K190016/annotation2011504/range2011504/1
type: Range
persistent identity: range2011504
display id: range2011504
version: 1
top level: BBa_K190016
direction: direction/forward
start: 32
end: 53
orientation: inline

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011503
display id: annotation2011503
version: 1
top level: BBa_K190016
location: https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K190016/annotation2011503/range2011503/1
type: Range
persistent identity: range2011503
display id: range2011503
version: 1
top level: BBa_K190016
direction: direction/forward
start: 35
end: 69
orientation: inline

https://synbiohub.org/public/igem/BBa_K190034_sequence/1
type: Sequence
persistent identity: BBa K190034 sequence
display id: BBa_K190034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaattactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I750016_sequence/1
type: Sequence
persistent identity: BBa I750016 sequence
display id: BBa_I750016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K190016_sequence/1
type: Sequence
persistent identity: BBa K190016 sequence
display id: BBa_K190016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z