LOCUS BBa_K190035 6115 bp DNA linear 14-Nov-2024 DEFINITION GVP with CueO regulated promoter ACCESSION BBa_K190035 VERSION BBa_K190035.1 FEATURES Location/Qualifiers promoter 1..43 /label=-35 and -10 region misc_feature 3..28 /label=CueR binding site promoter 1..43 /label=BBa_K190017 RBS 52..63 /label=b0034 CDS 70..336 /label=gvpB CDS 412..678 /label=gvpR CDS 707..1634 /label=gvpN CDS 1648..2415 /label=gvpF CDS 2415..2683 /label=gvpG CDS 2684..3893 /label=gvpL CDS 3911..4198 /label=gvpS CDS 4152..4436 /label=gvpK CDS 4458..4760 /label=gvpJ CDS 4797..5675 /label=gvpT CDS 5699..6115 /label=gvpU misc_feature 2896..2896 /label=gvpL g213a misc_feature 3001..3001 /label=gvpL g318a misc_feature 3034..3034 /label=gvpL g351a misc_feature 3379..3379 /label=gvpL g696a misc_feature 3395..3794 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 769..769 /label=c->a misc_feature 2754..2754 /label=t->c misc_feature 2823..2823 /label=t->c misc_feature 3273..3273 /label=t->a misc_feature 4583..4583 /label=t->a misc_feature 5121..5121 /label=g->a misc_feature 5184..5184 /label=g->a misc_feature 3355..3394 /label=First copy of 40bp section misc_feature 52..6115 /label=BBa_I750016 ORIGIN 1 ttgaccttcc cgtaagggga aggactatgc tcaacgtttg atttactaga gaaagaggag 61 aaatactaga tgtctattca aaaaagtact aatagttcaa gtttagcaga agtcattgac 121 cgtattttag ataaaggaat tgttattgat gcttttgcaa gagtttctgt tgtaggaatt 181 gaaattttaa cgattgaagc gcgagtggtt attgccagtg ttgatacatg gttacgctat 241 gcagaagcag tagggcttct tcgtgacgac gtagaagaaa acggtcttcc tgaacgttca 301 aattcaagtg aagggcagcc gcgttttagt atttaatatg aagtggtatg gaaccttcct 361 tttttgaggg tggttccagc ttttagatct aactattgga ggctactaaa aatggaaatt 421 aaaaaaatta tgcaagccgt gaacgacttt ttcggtgaac acgtagctcc tcctcataaa 481 attacctcgg tggaagctac tgaagatgaa ggttggagag ttattgttga agtcattgaa 541 gaacgagaat atatgaaaaa atacgccaaa gatgaaatgc tcggaacgta cgagtgcttt 601 gtaaataaag aaaaagaagt catttcattc aaacgactcg acgtcagata tagaagcgcc 661 attggcattg aagcataaac gataagatgg caggaggaac gtaaaaatga ccgtcttaac 721 agacaaaagg aaaaaaggca gtggagcttt tatacaagat gacgagacaa aagaggttct 781 ttcaagagcg ctgagctatt taaaatccgg ctattccatt cattttacag gtcctgccgg 841 cggaggcaaa acctctttag cgcgagcgct tgctaaaaag agaaagcgtc ctgtaatgct 901 gatgcacggg aatcacgagc tcaacaacaa agatttaatt ggcgatttta cgggatacac 961 gagcaaaaaa gtaatcgacc agtacgttcg ttctgtctat aaaaaagatg aacaggtgag 1021 tgaaaactgg caggatggcc gattgcttga agctgtaaaa aatggctata cgctgattta 1081 cgacgaattt actcgttcta agcctgcgac gaataatatc tttctatcga tattagaaga 1141 aggcgtgctg ccgctgtatg gagtaaaaat gaccgatcct tttgtgcgcg tgcatcccga 1201 tttccgcgtc atcttcacaa gcaatccagc tgagtatgcc ggcgtatatg atacgcaaga 1261 tgcgcttctc gacaggttaa ttaccatgtt tattgattat aaagacatcg acagagagac 1321 agcgatttta acggagaaaa cggacgtaga agaagatgaa gcgcgcacaa ttgtaacgct 1381 cgtagcaaac gtgcgaaacc gctctggaga cgaaaacagc agcggactta gcctgcgggc 1441 ttcgcttatg atcgctaccc ttgccacgca gcaagacatt cctatcgatg gaagtgacga 1501 agattttcaa acgttatgta tcgatatttt gcatcatccg cttaccaaat gtttggatga 1561 agaaaatgca aaaagcaaag ccgaaaaaat cattttagaa gaatgtaaga atatagacac 1621 tgaagaaaag taaaggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt 1681 tttagcgcca ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca 1741 aaagctgaaa catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg 1801 atgaaaattt atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg 1861 attatggaca agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa 1921 gaagacgtaa aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg 1981 atcaaaggaa aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa 2041 aaagtaaacg aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa 2101 gcagccggtt attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc 2161 ctgcaaaaag aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca 2221 aaagcaaatg agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga 2281 gaagatgaag cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa 2341 gccgattttc attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa 2401 gtagaagaga aataacgtgc ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa 2461 aatcggcgaa aaagtacagg aagaagctga taaacagcta tatgaccttc cgacgattca 2521 gcaaaagctc attcagcttc aaatgatgtt tgagcttggt gaaattccag aagaagcgtt 2581 tcaagaaaaa gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca gagaaattga 2641 acaatgggaa gagctaacac aaaaaagaaa tgaggaatcc tagatgggag aattactgta 2701 tttatacggt ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa 2761 ggggtttgac ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt 2821 ttctaagctg gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga 2881 tatgagctgg ctgcaagaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga 2941 agaatttacg atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca 3001 agcagctatt gagattaaca aagaaaagat agaaaattca ctgacgctgc ttcaaggaaa 3061 tgaagagtgg aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga 3121 aacgaatgaa agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca 3181 gttttttgaa aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa 3241 caaagtgtgt gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa 3301 aaagaattgg agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt 3361 tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa 3421 ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt 3481 tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa 3541 ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt 3601 tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa 3661 ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt 3721 tcttctcccg tctctgcaaa ttactaagtt cgtacgtgtt tcttctcccg tctctgcaaa 3781 ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat aaaggctgga 3841 agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg taaagtgagg 3901 aattaacatt atgtctctta aacaatccat ggagaataaa gatattgctc ttattgatat 3961 tttagatgtc attttagata aaggagtcgc cattaaagga gacttaatca tttccatagc 4021 tggcgtcgat ttagtgtatt tggatttgcg ggtgcttatt tcttcggttg aaacgcttgt 4081 gcaagcaaaa gaaggaaatc acaaaccaat cacttctgaa caatttgata aacaaaagga 4141 ggaattaatg gatgcaaccg gtcagccaag caaatggacg aatccacttg gatcctgatc 4201 aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg aggcaaatcg 4261 ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa caaattgaaa 4321 acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg 4381 gtctggatgc agaagattta aatattgatc ttggaccgct aggcagcctg ctttaagcgg 4441 tcagtaggag gaacagtatg gcagtcgaac ataatatgca gtcaagtacg attgtagatg 4501 tgctcgaaaa gattttggat aaaggagtcg ttatagcggg ggacatcacc gtaggaattg 4561 cagatgtcga gctattaacg ataaagatcc gcttgattgt ggcttcggtt gataaggcaa 4621 aagaaatcgg catggactgg tgggaaaatg atccgtatct cagttcaaaa ggagccaata 4681 acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa 4741 aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa aatggaggga tttacaatgg 4801 caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa 4861 acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca 4921 aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa 4981 atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt 5041 taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg 5101 gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt 5161 ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc 5221 aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta 5281 gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg 5341 atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta 5401 agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca 5461 gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag 5521 aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag 5581 ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa 5641 catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat 5701 gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc 5761 taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg 5821 taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag 5881 tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa 5941 cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag 6001 taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg 6061 gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataa //