BBa_K190035 1 BBa_K190035 GVP with CueO regulated promoter 2009-09-06T11:00:00Z 2015-05-08T01:11:15Z E.coli and Bacillus The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190017 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation. false false _306_ 0 4144 9 It's complicated false Melbourne GVP cluster was used with 400bp repeat in gvpL false Michael Verhoeven component2019602 1 BBa_I750016 component2019576 1 BBa_K190017 annotation2019602 1 BBa_I750016 range2019602 1 52 6115 annotation2019576 1 BBa_K190017 range2019576 1 1 43 BBa_K190017 1 BBa_K190017 Copper Promoter (CueR regulated) 2009-07-14T11:00:00Z 2015-05-08T01:11:15Z E.coli TOP10 Promoter sequence with recognition site for CueR transcriptional regulator protein. false false _306_ 0 4144 9 It's complicated false None false Michael Verhoeven annotation2011506 1 CueR binding site range2011506 1 3 28 annotation2011505 1 -35 and -10 region range2011505 1 1 43 BBa_I750016 1 BBa_I750016 Gas Vesicle polycistonic gene 2007-10-20T11:00:00Z 2015-08-31T04:08:05Z This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458). This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline. false false _132_ 0 1721 9 It's complicated false Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part. true Phillip Dodson annotation1951694 1 gvpL g213a range1951694 1 2845 2845 annotation1951683 1 gvpB range1951683 1 19 285 annotation1954616 1 First copy of 40bp section range1954616 1 3304 3343 annotation1954601 1 t->c range1954601 1 2772 2772 annotation1951698 1 gvpL g696a range1951698 1 3328 3328 annotation1951693 1 gvpU range1951693 1 5648 6064 annotation1951689 1 gvpS range1951689 1 3860 4147 annotation1951685 1 gvpN range1951685 1 656 1583 annotation1954604 1 g->a range1954604 1 5070 5070 annotation1951688 1 gvpL range1951688 1 2633 3842 annotation1951696 1 gvpL g318a range1951696 1 2950 2950 annotation1951692 1 gvpT range1951692 1 4746 5624 annotation1954602 1 t->a range1954602 1 3222 3222 annotation1954600 1 t->c range1954600 1 2703 2703 annotation1951690 1 gvpK range1951690 1 4101 4385 annotation1954598 1 10 repeats of 40bp induced durring mutagenesis range1954598 1 3344 3743 annotation1951682 1 b0034 range1951682 1 1 12 annotation1951686 1 gvpF range1951686 1 1597 2364 annotation1951691 1 gvpJ range1951691 1 4407 4709 annotation1951697 1 gvpL g351a range1951697 1 2983 2983 annotation1954603 1 t->a range1954603 1 4532 4532 annotation1954605 1 g->a range1954605 1 5133 5133 annotation1951687 1 gvpG range1951687 1 2364 2632 annotation1954599 1 c->a range1954599 1 718 718 annotation1951684 1 gvpR range1951684 1 361 627 BBa_K190017_sequence 1 ttgaccttcccgtaaggggaaggactatgctcaacgtttgatt BBa_I750016_sequence 1 aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataa BBa_K190035_sequence 1 ttgaccttcccgtaaggggaaggactatgctcaacgtttgatttactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z