##gff-version 3
##sequence-region BBa_K190035 1 6115
BBa_K190035	.	promoter	1	43	.	+	0	ID=BBa_K190017;Name=BBa_K190017
BBa_K190035	.	promoter	1	43	.	+	0	ID=annotation2011505;Name=-35 and -10 region;Parent=BBa_K190017
BBa_K190035	.	non_covalent_binding_site	3	28	.	+	0	ID=annotation2011506;Name=CueR binding site;Parent=BBa_K190017
BBa_K190035	.	engineered_region	52	6115	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_K190035	.	point_mutation	5121	5121	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_K190035	.	CDS	2684	3893	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_K190035	.	point_mutation	2823	2823	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_K190035	.	CDS	4797	5675	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_K190035	.	point_mutation	3379	3379	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_K190035	.	point_mutation	2896	2896	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_K190035	.	CDS	707	1634	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_K190035	.	sequence_alteration	3273	3273	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_K190035	.	CDS	5699	6115	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_K190035	.	CDS	1648	2415	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
BBa_K190035	.	CDS	70	336	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_K190035	.	point_mutation	3001	3001	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_K190035	.	sequence_feature	3395	3794	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_K190035	.	point_mutation	5184	5184	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_K190035	.	point_mutation	2754	2754	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_K190035	.	CDS	4458	4760	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_K190035	.	CDS	3911	4198	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_K190035	.	CDS	4152	4436	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
BBa_K190035	.	ribosome_entry_site	52	63	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_K190035	.	point_mutation	3034	3034	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_K190035	.	sequence_feature	3355	3394	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_K190035	.	point_mutation	4583	4583	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_K190035	.	point_mutation	769	769	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_K190035	.	CDS	412	678	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_K190035	.	CDS	2415	2683	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
>BBa_K190035
ttgaccttcccgtaaggggaaggactatgctcaacgtttgatttactagagaaagaggagaaatactagatgtctattc
aaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgc
aagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgc
tatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggc
agccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaacta
ttggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcct
cataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatata
tgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaa
acgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgac
cgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcg
ctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgc
ttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattt
tacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaac
tggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctg
cgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgt
gcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcg
cttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacgg
acgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcag
cggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaa
gattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaag
ccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaaca
aacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaa
caaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcc
taatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagc
tttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttc
cagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaa
tcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttgga
ggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcgg
aagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagc
gaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcct
gcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaac
cttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagc
tcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaat
gaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatg
ggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggt
ttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacaccta
ttcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacg
gtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaag
cagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaat
ttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagt
cacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaa
acaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaaga
cgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgt
gtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacg
tgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtac
gtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgta
cgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgt
acgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcg
taaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgcccta
tcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctc
ttattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcga
tttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaacca
atcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccac
ttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaac
gtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaactt
agaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggc
agcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgc
tcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgat
aaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctc
agttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaa
aaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattaga
taacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaa
acaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctg
aaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaa
agaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaa
gatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaag
aaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaa
tcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcag
gacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtg
acgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacga
agatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaag
aaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggagg
aaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaa
cacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatact
ttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcga
agcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaa
tctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctg
atgcaaaatcaacgagtaaaaagagttcataa