LOCUS BBa_K190036 6272 bp DNA linear 05-Nov-2024 DEFINITION GVP with LacI regulated promoter ACCESSION BBa_K190036 VERSION BBa_K190036.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..220 /label=b0034 CDS 227..493 /label=gvpB CDS 569..835 /label=gvpR CDS 864..1791 /label=gvpN CDS 1805..2572 /label=gvpF CDS 2572..2840 /label=gvpG CDS 2841..4050 /label=gvpL CDS 4068..4355 /label=gvpS CDS 4309..4593 /label=gvpK CDS 4615..4917 /label=gvpJ CDS 4954..5832 /label=gvpT CDS 5856..6272 /label=gvpU misc_feature 3053..3053 /label=gvpL g213a misc_feature 3158..3158 /label=gvpL g318a misc_feature 3191..3191 /label=gvpL g351a misc_feature 3536..3536 /label=gvpL g696a misc_feature 3552..3951 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 926..926 /label=c->a misc_feature 2911..2911 /label=t->c misc_feature 2980..2980 /label=t->c misc_feature 3430..3430 /label=t->a misc_feature 4740..4740 /label=t->a misc_feature 5278..5278 /label=g->a misc_feature 5341..5341 /label=g->a misc_feature 3512..3551 /label=First copy of 40bp section misc_feature 209..6272 /label=BBa_I750016 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctattcaaaa 241 aagtactaat agttcaagtt tagcagaagt cattgaccgt attttagata aaggaattgt 301 tattgatgct tttgcaagag tttctgttgt aggaattgaa attttaacga ttgaagcgcg 361 agtggttatt gccagtgttg atacatggtt acgctatgca gaagcagtag ggcttcttcg 421 tgacgacgta gaagaaaacg gtcttcctga acgttcaaat tcaagtgaag ggcagccgcg 481 ttttagtatt taatatgaag tggtatggaa ccttcctttt ttgagggtgg ttccagcttt 541 tagatctaac tattggaggc tactaaaaat ggaaattaaa aaaattatgc aagccgtgaa 601 cgactttttc ggtgaacacg tagctcctcc tcataaaatt acctcggtgg aagctactga 661 agatgaaggt tggagagtta ttgttgaagt cattgaagaa cgagaatata tgaaaaaata 721 cgccaaagat gaaatgctcg gaacgtacga gtgctttgta aataaagaaa aagaagtcat 781 ttcattcaaa cgactcgacg tcagatatag aagcgccatt ggcattgaag cataaacgat 841 aagatggcag gaggaacgta aaaatgaccg tcttaacaga caaaaggaaa aaaggcagtg 901 gagcttttat acaagatgac gagacaaaag aggttctttc aagagcgctg agctatttaa 961 aatccggcta ttccattcat tttacaggtc ctgccggcgg aggcaaaacc tctttagcgc 1021 gagcgcttgc taaaaagaga aagcgtcctg taatgctgat gcacgggaat cacgagctca 1081 acaacaaaga tttaattggc gattttacgg gatacacgag caaaaaagta atcgaccagt 1141 acgttcgttc tgtctataaa aaagatgaac aggtgagtga aaactggcag gatggccgat 1201 tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga cgaatttact cgttctaagc 1261 ctgcgacgaa taatatcttt ctatcgatat tagaagaagg cgtgctgccg ctgtatggag 1321 taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt ccgcgtcatc ttcacaagca 1381 atccagctga gtatgccggc gtatatgata cgcaagatgc gcttctcgac aggttaatta 1441 ccatgtttat tgattataaa gacatcgaca gagagacagc gattttaacg gagaaaacgg 1501 acgtagaaga agatgaagcg cgcacaattg taacgctcgt agcaaacgtg cgaaaccgct 1561 ctggagacga aaacagcagc ggacttagcc tgcgggcttc gcttatgatc gctacccttg 1621 ccacgcagca agacattcct atcgatggaa gtgacgaaga ttttcaaacg ttatgtatcg 1681 atattttgca tcatccgctt accaaatgtt tggatgaaga aaatgcaaaa agcaaagccg 1741 aaaaaatcat tttagaagaa tgtaagaata tagacactga agaaaagtaa aggagcttga 1801 aaacatgagt gaaacaaacg aaacaggtat ttatattttt agcgccattc aaacggataa 1861 agacgaagaa tttggcgccg tggaagtaga aggaacaaaa gctgaaacat ttttgattcg 1921 ctacaaagac gcggctatgg tagcagctga agtaccgatg aaaatttatc atcctaatcg 1981 ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt atggacaaga acgatacggt 2041 tattccaatc agctttggga atgtattcaa atcaaaagaa gacgtaaaag ttcttttgga 2101 aaacctttat ccgcagtttg aaaagctgtt tccagcgatc aaaggaaaaa ttgaagtcgg 2161 tttaaaagta attgggaaaa aagaatggct tgagaaaaaa gtaaacgaaa atcctgaact 2221 tgagaaagta tcagcatccg taaaaggaaa atcagaagca gccggttatt atgagcgtat 2281 tcaacttgga ggaatggctc aaaagatgtt tacttccctg caaaaagaag tcaagacaga 2341 tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa gcaaatgagc caacgggcga 2401 aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa gatgaagcga agtttgatga 2461 aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc gattttcatt acagcggtcc 2521 ttggcctgct tataattttg tgaacattcg cctaaaagta gaagagaaat aacgtgcttc 2581 acaaattagt aaccgcaccc attaaccttg tagtgaaaat cggcgaaaaa gtacaggaag 2641 aagctgataa acagctatat gaccttccga cgattcagca aaagctcatt cagcttcaaa 2701 tgatgtttga gcttggtgaa attccagaag aagcgtttca agaaaaagaa gatgaattgt 2761 taatgaggta cgaaattgcg aaacgcagag aaattgaaca atgggaagag ctaacacaaa 2821 aaagaaatga ggaatcctag atgggagaat tactgtattt atacggttta attccaacaa 2881 aagaagcagc agccatagag ccgtttccat cttataaggg gtttgacgga gaacattcac 2941 tgtacccaat tgcgtttgat caggtgacgg ctgtagtttc taagctggat gctgacacct 3001 attcagaaaa agtgattcaa gaaaaaatgg agcaggatat gagctggctg caagaaaaag 3061 catttcatca tcacgaaacg gtagccgctt tgtacgaaga atttacgatc attccattaa 3121 aattttgcac catttataaa ggtgaagaaa gtctgcaagc agctattgag attaacaaag 3181 aaaagataga aaattcactg acgctgcttc aaggaaatga agagtggaat gtgaaaattt 3241 actgtgatga tacagagctt aaaaaaggaa tcagcgaaac gaatgaaagc gtgaaagcga 3301 aaaaacaaga aattagtcac ttatcaccag gaagacagtt ttttgaaaag aaaaaaatag 3361 atcagctgat tgaaaaagaa ttagagcttc acaaaaacaa agtgtgtgaa gagatacatg 3421 acaagctaaa agaattatcg ctttatgact ctgttaaaaa gaattggagc aaagacgtaa 3481 ctggcgcagc tgaacagatg gcgtggaaca gcgtgtttct tctcccgtct ctgcaaatta 3541 ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct 3601 tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta 3661 ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct 3721 tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta 3781 ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct 3841 tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta 3901 ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt aaacgaaata 3961 gaagagcttc agcaaaggct tgaaaataaa ggctggaagt ttgaagtgac gggaccatgg 4021 ccgccctatc atttctcgag ctttgcgtaa agtgaggaat taacattatg tctcttaaac 4081 aatccatgga gaataaagat attgctctta ttgatatttt agatgtcatt ttagataaag 4141 gagtcgccat taaaggagac ttaatcattt ccatagctgg cgtcgattta gtgtatttgg 4201 atttgcgggt gcttatttct tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca 4261 aaccaatcac ttctgaacaa tttgataaac aaaaggagga attaatggat gcaaccggtc 4321 agccaagcaa atggacgaat ccacttggat cctgatcaag ctgaacaagg cttagcgcag 4381 cttgtgatga cagttattga gctattgagg caaatcgttg aacgtcatgc catgaggcgg 4441 gtggagggtg gaacgttgac ggacgaacaa attgaaaact taggaattgc actaatgaac 4501 ttagaagaaa aaatggacga gttgaaagag gtgttcggtc tggatgcaga agatttaaat 4561 attgatcttg gaccgctagg cagcctgctt taagcggtca gtaggaggaa cagtatggca 4621 gtcgaacata atatgcagtc aagtacgatt gtagatgtgc tcgaaaagat tttggataaa 4681 ggagtcgtta tagcggggga catcaccgta ggaattgcag atgtcgagct attaacgata 4741 aagatccgct tgattgtggc ttcggttgat aaggcaaaag aaatcggcat ggactggtgg 4801 gaaaatgatc cgtatctcag ttcaaaagga gccaataaca aagcgctcga agaagaaaat 4861 aaaatgctgc atgagcggtt aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa 4921 ctgtacgcta cttaaaaaat ggagggattt acaatggcaa ctgaaacaaa attagataac 4981 acacaggcag aaaacaagga aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt 5041 tcaaaagcaa gcaaaacaac aagcagcggg ccaatcaaac gagcggtagc aggaggcatc 5101 atcggtgcaa cgattggata tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc 5161 attgatacag acgaattaaa aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa 5221 tcaaaaagca gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa 5281 gataaatcaa aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat 5341 aacgttcaag agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca 5401 cagcttgaag aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa 5461 gaagcggaag atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa 5521 aacgagcagg acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa 5581 gaagctgagg aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct 5641 cgctcaaaca aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa 5701 gaagaaaaaa aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa 5761 gcaaagaaaa acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat 5821 acaacagcct aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa 5881 ctaaagacaa tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg 5941 atatttcatt aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat 6001 actttgatta cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg 6061 aacaattctc tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta 6121 ttcatttgaa aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca 6181 aaatcttttg gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg 6241 atgcaaaatc aacgagtaaa aagagttcat aa //