##gff-version 3
##sequence-region BBa_K190036 1 6272
BBa_K190036	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K190036	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K190036	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K190036	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K190036	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K190036	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K190036	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K190036	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K190036	.	engineered_region	209	6272	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_K190036	.	point_mutation	2911	2911	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_K190036	.	CDS	4068	4355	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_K190036	.	ribosome_entry_site	209	220	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_K190036	.	CDS	1805	2572	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
BBa_K190036	.	point_mutation	5341	5341	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_K190036	.	point_mutation	3191	3191	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_K190036	.	point_mutation	4740	4740	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_K190036	.	point_mutation	926	926	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_K190036	.	CDS	227	493	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_K190036	.	CDS	4615	4917	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_K190036	.	point_mutation	3158	3158	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_K190036	.	point_mutation	3536	3536	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_K190036	.	point_mutation	2980	2980	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_K190036	.	sequence_feature	3552	3951	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_K190036	.	CDS	2572	2840	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
BBa_K190036	.	CDS	2841	4050	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_K190036	.	CDS	5856	6272	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_K190036	.	point_mutation	5278	5278	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_K190036	.	CDS	864	1791	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_K190036	.	point_mutation	3053	3053	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_K190036	.	sequence_alteration	3430	3430	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_K190036	.	CDS	4954	5832	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_K190036	.	CDS	569	835	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_K190036	.	sequence_feature	3512	3551	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_K190036	.	CDS	4309	4593	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
>BBa_K190036
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtctattca
aaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgca
agagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgct
atgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggca
gccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactat
tggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctc
ataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatat
gaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaa
cgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgacc
gtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgc
tgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgct
tgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgatttt
acgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaact
ggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgc
gacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtg
cgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgc
ttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacgga
cgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagc
ggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaag
attttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagc
cgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaa
acgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaac
aaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcct
aatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagct
ttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttcc
agcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaat
cctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttggag
gaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcgga
agcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcg
aagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcctg
cttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaacc
ttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagct
cattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatg
aggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgg
gagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggtt
tgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacacctat
tcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacgg
tagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagc
agctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatt
tactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtc
acttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaa
caaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaagac
gtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtg
tttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgt
gtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacg
tgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtac
gtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgta
cgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgt
aaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctat
catttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctct
tattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcgat
ttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaa
tcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccact
tggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacg
tcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaactta
gaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggca
gcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgct
cgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgata
aagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctca
gttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaa
aatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattagat
aacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaa
caacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctga
aaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaa
gaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaag
atgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaaga
aaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaat
caagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcagg
acgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtga
cgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaa
gatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaaga
aaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggagga
aagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaac
acggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactt
tgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaa
gcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaat
ctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctga
tgcaaaatcaacgagtaaaaagagttcataa