BBa_K190036
type: ComponentDefinition
persistent identity: BBa K190036
display id: BBa_K190036
version: 1
was generated by: iGEM to SBOL conversion
description: GVP with LacI regulated promoter
created: 2009-09-06T11:00:00Z
modified: 2015-05-08T01:11:15Z
mutable provenance: E.coli and Bacillus
owned by: james
owned by: myers
mutable description: The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the LacI and CAP regulated promoter BBa_R0010 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.
discontinued: false
dominant: false
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Melbourne GVP cluster was used containing the 400bp repeat in gvpL
star: false
creator: Michael Verhoeven
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I750016
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_I750016
sequence: https://synbiohub.org/public/igem/BBa_K190036_sequence/1

BBa_I750016
type: Component
persistent identity: component2019635
display id: component2019635
version: 1
top level: BBa_K190036
definition: BBa_I750016
access: public

BBa_R0010
type: Component
persistent identity: component2019603
display id: component2019603
version: 1
top level: BBa_K190036
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2019603
display id: annotation2019603
version: 1
top level: BBa_K190036
location: https://synbiohub.org/public/igem/BBa_K190036/annotation2019603/range2019603/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K190036/annotation2019603/range2019603/1
type: Range
persistent identity: range2019603
display id: range2019603
version: 1
top level: BBa_K190036
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_I750016
type: SequenceAnnotation
persistent identity: annotation2019635
display id: annotation2019635
version: 1
top level: BBa_K190036
location: https://synbiohub.org/public/igem/BBa_K190036/annotation2019635/range2019635/1
component: BBa_I750016

https://synbiohub.org/public/igem/BBa_K190036/annotation2019635/range2019635/1
type: Range
persistent identity: range2019635
display id: range2019635
version: 1
top level: BBa_K190036
direction: direction/reverse
start: 209
end: 6272
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_I750016
type: ComponentDefinition
persistent identity: BBa I750016
display id: BBa_I750016
version: 1
was generated by: iGEM to SBOL conversion
description: Gas Vesicle polycistonic gene
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458).
owned by: james
owned by: myers
mutable description: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.
discontinued: false
dominant: false
experience: experience/None
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part.
star: true
creator: Phillip Dodson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: b0034
sequence annotation: gvpB
sequence annotation: gvpR
sequence annotation: gvpN
sequence annotation: c->a
sequence annotation: gvpF
sequence annotation: gvpG
sequence annotation: gvpL
sequence annotation: t->c
sequence annotation: gvpL g213a
sequence annotation: gvpL g318a
sequence annotation: gvpL g351a
sequence annotation: t->a
sequence annotation: First copy of 40bp section
sequence annotation: gvpL g696a
sequence annotation: 10 repeats of 40bp induced durring mutagenesis
sequence annotation: gvpS
sequence annotation: gvpK
sequence annotation: gvpJ
sequence annotation: gvpT
sequence annotation: g->a
sequence annotation: gvpU
sequence: https://synbiohub.org/public/igem/BBa_I750016_sequence/1

b0034
type: SequenceAnnotation
persistent identity: annotation1951682
display id: annotation1951682
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
type: Range
persistent identity: range1951682
display id: range1951682
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1
end: 12
orientation: inline

gvpB
type: SequenceAnnotation
persistent identity: annotation1951683
display id: annotation1951683
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
type: Range
persistent identity: range1951683
display id: range1951683
version: 1
top level: BBa_I750016
direction: direction/forward
start: 19
end: 285
orientation: inline

gvpR
type: SequenceAnnotation
persistent identity: annotation1951684
display id: annotation1951684
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
type: Range
persistent identity: range1951684
display id: range1951684
version: 1
top level: BBa_I750016
direction: direction/forward
start: 361
end: 627
orientation: inline

gvpN
type: SequenceAnnotation
persistent identity: annotation1951685
display id: annotation1951685
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
type: Range
persistent identity: range1951685
display id: range1951685
version: 1
top level: BBa_I750016
direction: direction/forward
start: 656
end: 1583
orientation: inline

c->a
type: SequenceAnnotation
persistent identity: annotation1954599
display id: annotation1954599
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
type: Range
persistent identity: range1954599
display id: range1954599
version: 1
top level: BBa_I750016
direction: direction/forward
start: 718
end: 718
orientation: inline

gvpF
type: SequenceAnnotation
persistent identity: annotation1951686
display id: annotation1951686
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
type: Range
persistent identity: range1951686
display id: range1951686
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1597
end: 2364
orientation: inline

gvpG
type: SequenceAnnotation
persistent identity: annotation1951687
display id: annotation1951687
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
type: Range
persistent identity: range1951687
display id: range1951687
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2364
end: 2632
orientation: inline

gvpL
type: SequenceAnnotation
persistent identity: annotation1951688
display id: annotation1951688
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
type: Range
persistent identity: range1951688
display id: range1951688
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2633
end: 3842
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954600
display id: annotation1954600
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
type: Range
persistent identity: range1954600
display id: range1954600
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2703
end: 2703
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954601
display id: annotation1954601
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
type: Range
persistent identity: range1954601
display id: range1954601
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2772
end: 2772
orientation: inline

gvpL g213a
type: SequenceAnnotation
persistent identity: annotation1951694
display id: annotation1951694
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
type: Range
persistent identity: range1951694
display id: range1951694
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2845
end: 2845
orientation: inline

gvpL g318a
type: SequenceAnnotation
persistent identity: annotation1951696
display id: annotation1951696
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
type: Range
persistent identity: range1951696
display id: range1951696
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2950
end: 2950
orientation: inline

gvpL g351a
type: SequenceAnnotation
persistent identity: annotation1951697
display id: annotation1951697
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
type: Range
persistent identity: range1951697
display id: range1951697
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2983
end: 2983
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954602
display id: annotation1954602
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
type: Range
persistent identity: range1954602
display id: range1954602
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3222
end: 3222
orientation: inline

First copy of 40bp section
type: SequenceAnnotation
persistent identity: annotation1954616
display id: annotation1954616
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
type: Range
persistent identity: range1954616
display id: range1954616
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3304
end: 3343
orientation: inline

gvpL g696a
type: SequenceAnnotation
persistent identity: annotation1951698
display id: annotation1951698
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
type: Range
persistent identity: range1951698
display id: range1951698
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3328
end: 3328
orientation: inline

10 repeats of 40bp induced durring mutagenesis
type: SequenceAnnotation
persistent identity: annotation1954598
display id: annotation1954598
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
type: Range
persistent identity: range1954598
display id: range1954598
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3344
end: 3743
orientation: inline

gvpS
type: SequenceAnnotation
persistent identity: annotation1951689
display id: annotation1951689
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
type: Range
persistent identity: range1951689
display id: range1951689
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3860
end: 4147
orientation: inline

gvpK
type: SequenceAnnotation
persistent identity: annotation1951690
display id: annotation1951690
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
type: Range
persistent identity: range1951690
display id: range1951690
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4101
end: 4385
orientation: inline

gvpJ
type: SequenceAnnotation
persistent identity: annotation1951691
display id: annotation1951691
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
type: Range
persistent identity: range1951691
display id: range1951691
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4407
end: 4709
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954603
display id: annotation1954603
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
type: Range
persistent identity: range1954603
display id: range1954603
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4532
end: 4532
orientation: inline

gvpT
type: SequenceAnnotation
persistent identity: annotation1951692
display id: annotation1951692
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
type: Range
persistent identity: range1951692
display id: range1951692
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4746
end: 5624
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954604
display id: annotation1954604
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
type: Range
persistent identity: range1954604
display id: range1954604
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5070
end: 5070
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954605
display id: annotation1954605
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
type: Range
persistent identity: range1954605
display id: range1954605
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5133
end: 5133
orientation: inline

gvpU
type: SequenceAnnotation
persistent identity: annotation1951693
display id: annotation1951693
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
type: Range
persistent identity: range1951693
display id: range1951693
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5648
end: 6064
orientation: inline

https://synbiohub.org/public/igem/BBa_K190036_sequence/1
type: Sequence
persistent identity: BBa K190036 sequence
display id: BBa_K190036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I750016_sequence/1
type: Sequence
persistent identity: BBa I750016 sequence
display id: BBa_I750016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacga…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z