LOCUS BBa_K190037 7282 bp DNA linear 14-Nov-2024 DEFINITION GVP with pBad/araC promoter ACCESSION BBa_K190037 VERSION BBa_K190037.1 FEATURES Location/Qualifiers promoter 1..1210 /label=BBa_K113009 RBS 1219..1230 /label=b0034 CDS 1237..1503 /label=gvpB CDS 1579..1845 /label=gvpR CDS 1874..2801 /label=gvpN CDS 2815..3582 /label=gvpF CDS 3582..3850 /label=gvpG CDS 3851..5060 /label=gvpL CDS 5078..5365 /label=gvpS CDS 5319..5603 /label=gvpK CDS 5625..5927 /label=gvpJ CDS 5964..6842 /label=gvpT CDS 6866..7282 /label=gvpU misc_feature 4063..4063 /label=gvpL g213a misc_feature 4168..4168 /label=gvpL g318a misc_feature 4201..4201 /label=gvpL g351a misc_feature 4546..4546 /label=gvpL g696a misc_feature 4562..4961 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 1936..1936 /label=c->a misc_feature 3921..3921 /label=t->c misc_feature 3990..3990 /label=t->c misc_feature 4440..4440 /label=t->a misc_feature 5750..5750 /label=t->a misc_feature 6288..6288 /label=g->a misc_feature 6351..6351 /label=g->a misc_feature 4522..4561 /label=First copy of 40bp section misc_feature 1219..7282 /label=BBa_I750016 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ctattcaaaa aagtactaat 1261 agttcaagtt tagcagaagt cattgaccgt attttagata aaggaattgt tattgatgct 1321 tttgcaagag tttctgttgt aggaattgaa attttaacga ttgaagcgcg agtggttatt 1381 gccagtgttg atacatggtt acgctatgca gaagcagtag ggcttcttcg tgacgacgta 1441 gaagaaaacg gtcttcctga acgttcaaat tcaagtgaag ggcagccgcg ttttagtatt 1501 taatatgaag tggtatggaa ccttcctttt ttgagggtgg ttccagcttt tagatctaac 1561 tattggaggc tactaaaaat ggaaattaaa aaaattatgc aagccgtgaa cgactttttc 1621 ggtgaacacg tagctcctcc tcataaaatt acctcggtgg aagctactga agatgaaggt 1681 tggagagtta ttgttgaagt cattgaagaa cgagaatata tgaaaaaata cgccaaagat 1741 gaaatgctcg gaacgtacga gtgctttgta aataaagaaa aagaagtcat ttcattcaaa 1801 cgactcgacg tcagatatag aagcgccatt ggcattgaag cataaacgat aagatggcag 1861 gaggaacgta aaaatgaccg tcttaacaga caaaaggaaa aaaggcagtg gagcttttat 1921 acaagatgac gagacaaaag aggttctttc aagagcgctg agctatttaa aatccggcta 1981 ttccattcat tttacaggtc ctgccggcgg aggcaaaacc tctttagcgc gagcgcttgc 2041 taaaaagaga aagcgtcctg taatgctgat gcacgggaat cacgagctca acaacaaaga 2101 tttaattggc gattttacgg gatacacgag caaaaaagta atcgaccagt acgttcgttc 2161 tgtctataaa aaagatgaac aggtgagtga aaactggcag gatggccgat tgcttgaagc 2221 tgtaaaaaat ggctatacgc tgatttacga cgaatttact cgttctaagc ctgcgacgaa 2281 taatatcttt ctatcgatat tagaagaagg cgtgctgccg ctgtatggag taaaaatgac 2341 cgatcctttt gtgcgcgtgc atcccgattt ccgcgtcatc ttcacaagca atccagctga 2401 gtatgccggc gtatatgata cgcaagatgc gcttctcgac aggttaatta ccatgtttat 2461 tgattataaa gacatcgaca gagagacagc gattttaacg gagaaaacgg acgtagaaga 2521 agatgaagcg cgcacaattg taacgctcgt agcaaacgtg cgaaaccgct ctggagacga 2581 aaacagcagc ggacttagcc tgcgggcttc gcttatgatc gctacccttg ccacgcagca 2641 agacattcct atcgatggaa gtgacgaaga ttttcaaacg ttatgtatcg atattttgca 2701 tcatccgctt accaaatgtt tggatgaaga aaatgcaaaa agcaaagccg aaaaaatcat 2761 tttagaagaa tgtaagaata tagacactga agaaaagtaa aggagcttga aaacatgagt 2821 gaaacaaacg aaacaggtat ttatattttt agcgccattc aaacggataa agacgaagaa 2881 tttggcgccg tggaagtaga aggaacaaaa gctgaaacat ttttgattcg ctacaaagac 2941 gcggctatgg tagcagctga agtaccgatg aaaatttatc atcctaatcg ccaaaattta 3001 ttaatgcatc aaaacgcagt agcagcgatt atggacaaga acgatacggt tattccaatc 3061 agctttggga atgtattcaa atcaaaagaa gacgtaaaag ttcttttgga aaacctttat 3121 ccgcagtttg aaaagctgtt tccagcgatc aaaggaaaaa ttgaagtcgg tttaaaagta 3181 attgggaaaa aagaatggct tgagaaaaaa gtaaacgaaa atcctgaact tgagaaagta 3241 tcagcatccg taaaaggaaa atcagaagca gccggttatt atgagcgtat tcaacttgga 3301 ggaatggctc aaaagatgtt tacttccctg caaaaagaag tcaagacaga tgtgttttct 3361 ccgcttgaag aagcagcgga agcagcaaaa gcaaatgagc caacgggcga aacgatgctt 3421 ttaaacgcgt ctttcttaat taaccgagaa gatgaagcga agtttgatga aaaagtaaat 3481 gaagcgcatg aaaactggaa agacaaagcc gattttcatt acagcggtcc ttggcctgct 3541 tataattttg tgaacattcg cctaaaagta gaagagaaat aacgtgcttc acaaattagt 3601 aaccgcaccc attaaccttg tagtgaaaat cggcgaaaaa gtacaggaag aagctgataa 3661 acagctatat gaccttccga cgattcagca aaagctcatt cagcttcaaa tgatgtttga 3721 gcttggtgaa attccagaag aagcgtttca agaaaaagaa gatgaattgt taatgaggta 3781 cgaaattgcg aaacgcagag aaattgaaca atgggaagag ctaacacaaa aaagaaatga 3841 ggaatcctag atgggagaat tactgtattt atacggttta attccaacaa aagaagcagc 3901 agccatagag ccgtttccat cttataaggg gtttgacgga gaacattcac tgtacccaat 3961 tgcgtttgat caggtgacgg ctgtagtttc taagctggat gctgacacct attcagaaaa 4021 agtgattcaa gaaaaaatgg agcaggatat gagctggctg caagaaaaag catttcatca 4081 tcacgaaacg gtagccgctt tgtacgaaga atttacgatc attccattaa aattttgcac 4141 catttataaa ggtgaagaaa gtctgcaagc agctattgag attaacaaag aaaagataga 4201 aaattcactg acgctgcttc aaggaaatga agagtggaat gtgaaaattt actgtgatga 4261 tacagagctt aaaaaaggaa tcagcgaaac gaatgaaagc gtgaaagcga aaaaacaaga 4321 aattagtcac ttatcaccag gaagacagtt ttttgaaaag aaaaaaatag atcagctgat 4381 tgaaaaagaa ttagagcttc acaaaaacaa agtgtgtgaa gagatacatg acaagctaaa 4441 agaattatcg ctttatgact ctgttaaaaa gaattggagc aaagacgtaa ctggcgcagc 4501 tgaacagatg gcgtggaaca gcgtgtttct tctcccgtct ctgcaaatta ctaagttcgt 4561 acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct 4621 ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt 4681 acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct 4741 ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt 4801 acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct 4861 ctgcaaatta ctaagttcgt acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt 4921 acgtgtttct tctcccgtct ctgcaaatta ctaagttcgt aaacgaaata gaagagcttc 4981 agcaaaggct tgaaaataaa ggctggaagt ttgaagtgac gggaccatgg ccgccctatc 5041 atttctcgag ctttgcgtaa agtgaggaat taacattatg tctcttaaac aatccatgga 5101 gaataaagat attgctctta ttgatatttt agatgtcatt ttagataaag gagtcgccat 5161 taaaggagac ttaatcattt ccatagctgg cgtcgattta gtgtatttgg atttgcgggt 5221 gcttatttct tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca aaccaatcac 5281 ttctgaacaa tttgataaac aaaaggagga attaatggat gcaaccggtc agccaagcaa 5341 atggacgaat ccacttggat cctgatcaag ctgaacaagg cttagcgcag cttgtgatga 5401 cagttattga gctattgagg caaatcgttg aacgtcatgc catgaggcgg gtggagggtg 5461 gaacgttgac ggacgaacaa attgaaaact taggaattgc actaatgaac ttagaagaaa 5521 aaatggacga gttgaaagag gtgttcggtc tggatgcaga agatttaaat attgatcttg 5581 gaccgctagg cagcctgctt taagcggtca gtaggaggaa cagtatggca gtcgaacata 5641 atatgcagtc aagtacgatt gtagatgtgc tcgaaaagat tttggataaa ggagtcgtta 5701 tagcggggga catcaccgta ggaattgcag atgtcgagct attaacgata aagatccgct 5761 tgattgtggc ttcggttgat aaggcaaaag aaatcggcat ggactggtgg gaaaatgatc 5821 cgtatctcag ttcaaaagga gccaataaca aagcgctcga agaagaaaat aaaatgctgc 5881 atgagcggtt aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa ctgtacgcta 5941 cttaaaaaat ggagggattt acaatggcaa ctgaaacaaa attagataac acacaggcag 6001 aaaacaagga aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt tcaaaagcaa 6061 gcaaaacaac aagcagcggg ccaatcaaac gagcggtagc aggaggcatc atcggtgcaa 6121 cgattggata tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc attgatacag 6181 acgaattaaa aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa tcaaaaagca 6241 gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa gataaatcaa 6301 aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat aacgttcaag 6361 agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca cagcttgaag 6421 aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa gaagcggaag 6481 atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa aacgagcagg 6541 acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa gaagctgagg 6601 aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct cgctcaaaca 6661 aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa gaagaaaaaa 6721 aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa gcaaagaaaa 6781 acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat acaacagcct 6841 aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa ctaaagacaa 6901 tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg atatttcatt 6961 aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat actttgatta 7021 cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg aacaattctc 7081 tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta ttcatttgaa 7141 aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca aaatcttttg 7201 gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg atgcaaaatc 7261 aacgagtaaa aagagttcat aa //