BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K190038
1
BBa_K190038
Arsenic accumulation device (lactose promoter + fMT + GlpF)
2009-09-07T11:00:00Z
2015-05-08T01:11:15Z
The device is composed from BBa_K190032, which contains the lactose promoter (BBa_R0010) together with fMT ( BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.
A device which can be used to accumulate As(III) without (under certain concentrations) toxifying ''E. coli''. The expression of both genes is inducible by addition of IPTG.
false
false
_306_
0
4147
9
It's complicated
false
The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.
false
Nienke Kuipers
component2019670
1
BBa_R0010
component2019679
1
BBa_K190019
component2019681
1
BBa_K190028
annotation2019681
1
BBa_K190028
range2019681
1
437
1282
annotation2019670
1
BBa_R0010
range2019670
1
1
200
annotation2019679
1
BBa_K190019
range2019679
1
209
430
BBa_K190028
1
BBa_K190028
GlpF
2009-08-25T11:00:00Z
2015-05-08T01:11:15Z
This part comes from genomic DNA of the Escherichia coli 536.
Released HQ 2013
GlpF is an aquaglyceroporin channel that facilitates the transport of As(III).
false
false
_306_
0
4226
9
In stock
true
removal of an EcoR1 restiction site
false
Jolanda Witteveen
annotation2018038
1
start
range2018038
1
1
3
annotation2061212
1
GlpF
range2061212
1
1
846
BBa_K190019
1
BBa_K190019
fMT
2009-08-18T11:00:00Z
2016-01-13T10:21:29Z
fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.
fMT is a metallothionein binding Arsenite (III) and Arsenate(V), used to accumulate As when overexpressed. Binds As (III) stronger than As(V)
Reference: Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924???2927
false
false
_306_
4206
4147
9
In stock
true
Add BioBrick prefix and suffix, add RBS (B0034)
false
Nienke Kuipers
annotation2017543
1
fMT
range2017543
1
19
222
annotation2017542
1
B0034`
range2017542
1
1
18
BBa_K190038_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcgggcactggctgcaagatctgggaagactgcaagtgcggagcggcgtgcagctgcggcgactcgtgcacctgcggaactgtcaagaagggcaccacctctcgcgccggcgcgggctgcccctgcggccccaagtgcaaatgcaccggccaaggcagctgcaactgcgtcaaggacgactgctgcggctgcggcaagtaatactagatgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K190019_sequence
1
aaagaggagaaatactagatggcgggcactggctgcaagatctgggaagactgcaagtgcggagcggcgtgcagctgcggcgactcgtgcacctgcggaactgtcaagaagggcaccacctctcgcgccggcgcgggctgcccctgcggccccaagtgcaaatgcaccggccaaggcagctgcaactgcgtcaaggacgactgctgcggctgcggcaagtaa
BBa_K190028_sequence
1
atgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z