BBa_K190028 1 BBa_K190028 GlpF 2009-08-25T11:00:00Z 2015-05-08T01:11:15Z This part comes from genomic DNA of the Escherichia coli 536. Released HQ 2013 GlpF is an aquaglyceroporin channel that facilitates the transport of As(III). false false _306_ 0 4226 9 In stock true removal of an EcoR1 restiction site false Jolanda Witteveen annotation2061212 1 GlpF range2061212 1 1 846 annotation2018038 1 start range2018038 1 1 3 BBa_J23109 1 BBa_J23109 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K190019 1 BBa_K190019 fMT 2009-08-18T11:00:00Z 2016-01-13T10:21:29Z fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus. fMT is a metallothionein binding Arsenite (III) and Arsenate(V), used to accumulate As when overexpressed. Binds As (III) stronger than As(V) Reference: Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924???2927 false false _306_ 4206 4147 9 In stock true Add BioBrick prefix and suffix, add RBS (B0034) false Nienke Kuipers annotation2017543 1 fMT range2017543 1 19 222 annotation2017542 1 B0034` range2017542 1 1 18 BBa_K190039 1 BBa_K190039 Arsenic Accumulation device (Low constitutive promoter + fMT + GlpF) 2009-09-07T11:00:00Z 2015-05-08T01:11:15Z The device is composed from BBa_K190031, which contains the low constitutive promoter (BBa_J23109) together with fMT (BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter. A device which can be used to accumulate As(III) without (under certain concentrations) toxifying E. coli. The expression of both genes is constitutively expressed (under low constitutive promoter). false false _306_ 0 4147 9 It's complicated false The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon. false Nienke Kuipers component2019682 1 BBa_J23109 component2019685 1 BBa_K190019 component2019687 1 BBa_K190028 annotation2019687 1 BBa_K190028 range2019687 1 272 1117 annotation2019685 1 BBa_K190019 range2019685 1 44 265 annotation2019682 1 BBa_J23109 range2019682 1 1 35 BBa_J23109_sequence 1 tttacagctagctcagtcctagggactgtgctagc BBa_K190039_sequence 1 tttacagctagctcagtcctagggactgtgctagctactagagaaagaggagaaatactagatggcgggcactggctgcaagatctgggaagactgcaagtgcggagcggcgtgcagctgcggcgactcgtgcacctgcggaactgtcaagaagggcaccacctctcgcgccggcgcgggctgcccctgcggccccaagtgcaaatgcaccggccaaggcagctgcaactgcgtcaaggacgactgctgcggctgcggcaagtaatactagatgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa BBa_K190019_sequence 1 aaagaggagaaatactagatggcgggcactggctgcaagatctgggaagactgcaagtgcggagcggcgtgcagctgcggcgactcgtgcacctgcggaactgtcaagaagggcaccacctctcgcgccggcgcgggctgcccctgcggccccaagtgcaaatgcaccggccaaggcagctgcaactgcgtcaaggacgactgctgcggctgcggcaagtaa BBa_K190028_sequence 1 atgagtcaaacatcaaccttgaaaggccagtgcattgctgaatttctcggtaccgggttgttgattttcttcggtgtgggttgcgttgcagcactaaaagtcgctggtgcgtcttttggtcagtgggaaatcagtgtcatttggggactgggggtggcaatggccatctacctgaccgcaggggtttccggcgcgcatcttaatcccgctgttaccattgcattgtggctgtttgcctgtttcgacaagcgcaaagttattccttttatcgtttcacaagttgccggcgctttctgtgctgcggctttagtttacgggctttactacaatttatttttcgacttcgagcagactcatcacattgttcgcggcagcgttgaaagtgttgatctggctggcactttctctacttaccctaatcctcatatcaattttgtgcaggctttcgcagttgagatggtgattaccgctattctgatggggctgatcctggcgttaacggacgatggcaacggtgtaccacgcggccctttggctcccttgctgattggtctactgattgcggtcattggcgcatctatgggcccattgacaggttttgccatgaacccagcgcgtgacttcggtccgaaagtctttgcctggctggcgggctggggcaatgtcgcctttaccggcggcagagacattccttacttcctggtgccgcttttcggccctatcgttggcgcgattgtaggtgcatttgcctaccgcaaactgattggtcgccatttgccttgcgatatctgtgttgtggaagaaaaggaaaccacaactccttcagaacaaaaagcttcgctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z