LOCUS       BBa_K190063             1072 bp    DNA     linear       22-Dec-2024
DEFINITION  GlpF with pLacI
ACCESSION   BBa_K190063
VERSION     BBa_K190063.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     CDS             227..1072
                     /label=GlpF
     CDS             227..1072
                     /label=BBa_K190028
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gtcaaacatc
      241 aaccttgaaa ggccagtgca ttgctgaatt tctcggtacc gggttgttga ttttcttcgg
      301 tgtgggttgc gttgcagcac taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag
      361 tgtcatttgg ggactggggg tggcaatggc catctacctg accgcagggg tttccggcgc
      421 gcatcttaat cccgctgtta ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa
      481 agttattcct tttatcgttt cacaagttgc cggcgctttc tgtgctgcgg ctttagttta
      541 cgggctttac tacaatttat ttttcgactt cgagcagact catcacattg ttcgcggcag
      601 cgttgaaagt gttgatctgg ctggcacttt ctctacttac cctaatcctc atatcaattt
      661 tgtgcaggct ttcgcagttg agatggtgat taccgctatt ctgatggggc tgatcctggc
      721 gttaacggac gatggcaacg gtgtaccacg cggccctttg gctcccttgc tgattggtct
      781 actgattgcg gtcattggcg catctatggg cccattgaca ggttttgcca tgaacccagc
      841 gcgtgacttc ggtccgaaag tctttgcctg gctggcgggc tggggcaatg tcgcctttac
      901 cggcggcaga gacattcctt acttcctggt gccgcttttc ggccctatcg ttggcgcgat
      961 tgtaggtgca tttgcctacc gcaaactgat tggtcgccat ttgccttgcg atatctgtgt
     1021 tgtggaagaa aaggaaacca caactccttc agaacaaaaa gcttcgctgt aa
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