LOCUS       BBa_K190073             2616 bp    DNA     linear       26-Dec-2024
DEFINITION  Arsenic Accumulation Device (able to regulate pArsR)
ACCESSION   BBa_K190073
VERSION     BBa_K190073.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1326
                     /label=MBP
     misc_feature    227..229
                     /label=start
     misc_feature    1327..1347
                     /label=TEV cleavage site
     CDS             1360..1710
                     /label=ArsR
     misc_feature    1711..1741
                     /label=His10
     misc_feature    1742..1744
                     /label=stop
     CDS             227..1744
                     /label=BBa_K190027
     misc_feature    1757..1760
                     /label=conserved
     RBS             1753..1764
                     /label=BBa_B0034
     misc_feature    1771..1773
                     /label=start
     CDS             1771..2616
                     /label=GlpF
     CDS             1771..2616
                     /label=BBa_K190028
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaatcgaaga
      241 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
      301 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
      361 agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca
      421 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
      481 gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat
      541 tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa
      601 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
      661 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
      721 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
      781 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
      841 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
      901 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
      961 aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc
     1021 aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct
     1081 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
     1141 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc
     1201 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg
     1261 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgcagccc tggcagctgc
     1321 gcagactgaa gagctctact tccagggtgc cgccgccgcc atgtcatttc tgttacccat
     1381 ccaattgttc aaaattcttg ctgatgaaac ccgtctgggc atcgttttac tgctcagcga
     1441 actgggagag ttatgcgtct gcgatctctg cactgctctc gaccagtcgc agcccaagat
     1501 ctcccgccac ctggcattgc tgcgtgaaag cgggctattg ctggaccgca agcaaggtaa
     1561 gtgggttcat taccgcttat caccgcatat tccagcatgg gcggcgaaaa ttattgatga
     1621 ggcctggcga tgtgaacagg aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa
     1681 ctgttccggg gacagtaaga acatttgcag tcatcatcat caccatcatc atcaccatca
     1741 ttaatactag agaaagagga gaaatactag atgagtcaaa catcaacctt gaaaggccag
     1801 tgcattgctg aatttctcgg taccgggttg ttgattttct tcggtgtggg ttgcgttgca
     1861 gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg
     1921 ggggtggcaa tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct
     1981 gttaccattg cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc
     2041 gtttcacaag ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat
     2101 ttatttttcg acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat
     2161 ctggctggca ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca
     2221 gttgagatgg tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc
     2281 aacggtgtac cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt
     2341 ggcgcatcta tgggcccatt gacaggtttt gccatgaacc cagcgcgtga cttcggtccg
     2401 aaagtctttg cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt
     2461 ccttacttcc tggtgccgct tttcggccct atcgttggcg cgattgtagg tgcatttgcc
     2521 taccgcaaac tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggaa
     2581 accacaactc cttcagaaca aaaagcttcg ctgtaa
//